Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,087,490 G→A 7.5% S440S (AGC→AGT)  astD ← N‑succinylglutamate 5‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,087,4900GA 7.5% 149.3 / 2.0 54S440S (AGC→AGT) astDN‑succinylglutamate 5‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base A (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

TGACGCCCGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGC  >  NC_002947/5087354‑5087636
                                                                                                                                        |                                                                                                                                                  
tGACGCCCGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGcc                                                                                                                                        >  8:227895/1‑149 (MQ=255)
  aCGCCCGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGc                                                                                                                                            <  8:171709/143‑1 (MQ=255)
  aCGCCCGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGc                                                                                                                                            >  7:171709/1‑143 (MQ=255)
      ccGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGt                                                                                                                                   >  4:220993/1‑148 (MQ=255)
      ccGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGt                                                                                                                                   >  8:263826/1‑148 (MQ=255)
       cGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGt                                                                                                                                   >  5:72370/1‑147 (MQ=255)
        ggCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCCGCGCCTGTCAg                                                                                                                                >  8:298596/1‑149 (MQ=255)
        ggCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAg                                                                                                                                <  1:214696/149‑1 (MQ=255)
        ggCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAg                                                                                                                                <  7:263826/149‑1 (MQ=255)
         gCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGc                                                                                                                               >  3:281260/1‑149 (MQ=255)
          cGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCt                                                                                                                              <  5:228160/149‑1 (MQ=255)
                  tCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTc                                                                                                                      <  6:342822/149‑1 (MQ=255)
                    gCCGGCAAGGCAATGCTGGCTGTCTCCAGCGAAGCCAGGGGGTAAGCGCAGTTGTCTGCTGCGTAATAGGCACTGGCGCGATGGTTTCCACTTGCTCCCACGCCACCGAACGGCGCTCTTCTGGCAGCCCCTGTCAGCTGTTTGTTCCa                                                                                                                    <  3:112353/149‑1 (MQ=255)
                    gCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCa                                                                                                                    <  4:288971/149‑1 (MQ=255)
                    gCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCa                                                                                                                    <  6:248851/149‑1 (MQ=255)
                      cGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGt                                                                                                                  <  4:256620/149‑1 (MQ=255)
                            ggcaaggcTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGa                                                                                                            >  7:64867/1‑149 (MQ=255)
                             gcaaggcTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGGTTGTTCCAGTTGACGAt                                                                                                           >  7:60449/1‑149 (MQ=255)
                               aaggcTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGc                                                                                                         >  5:143295/1‑149 (MQ=255)
                                     ggCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTgcgc                                                                                                   >  7:85937/1‑149 (MQ=255)
                                            tcCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTgcg                                                                                            >  8:314127/1‑149 (MQ=255)
                                             ccAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGccc                                                                                                                                       <  2:49921/105‑1 (MQ=255)
                                             ccAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGccc                                                                                                                                       >  1:49921/1‑105 (MQ=255)
                                              cAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCa                                                                                          <  8:85937/149‑1 (MQ=255)
                                                gCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGc                                                                                        <  6:143295/149‑1 (MQ=255)
                                                  gAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCa                                                                                      <  4:281260/149‑1 (MQ=255)
                                                         cGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTAc                                                                               <  8:64867/149‑1 (MQ=255)
                                                           gggTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGGCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTg                                                                             <  6:72370/149‑1 (MQ=255)
                                                                 gcgcAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCg                                                                       >  2:289769/1‑149 (MQ=255)
                                                                   gcAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCACTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCggg                                                                     >  5:194249/1‑149 (MQ=255)
                                                                             gCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAAt                                                            >  2:15869/1‑148 (MQ=255)
                                                                                 cGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGa                                                        <  1:289769/148‑1 (MQ=255)
                                                                                    aaTAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACaa                                                     >  1:155499/1‑148 (MQ=255)
                                                                                    aaTAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCACTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACaa                                                     >  7:268985/1‑148 (MQ=255)
                                                                                      tAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCACTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATaa                                                  <  8:268985/149‑1 (MQ=255)
                                                                                         gCACTGGCGCGATGGTTGACACTGGCTCCCACGCCACCCAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCAGCGAGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTACCGAATCAGACAATAAGcc                                               >  6:19231/1‑149 (MQ=255)
                                                                                                    aTGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCggccaggc                                    <  7:227895/149‑1 (MQ=255)
                                                                                                     tGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTTTCAGCTGTTTGTTCCAGTTGACGATGACTGCGCTACTGCGAAGCCAGAAGTAGTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCggccaggcc                                   <  5:19231/149‑1 (MQ=255)
                                                                                                              cTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGgt                          <  7:314127/149‑1 (MQ=255)
                                                                                                               tGGCTCCCACGCCACCGAACGGCGCACTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGaa                                                             <  5:99225/113‑1 (MQ=255)
                                                                                                               tGGCTCCCACGCCACCGAACGGCGCACTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGaa                                                             >  6:99225/1‑113 (MQ=255)
                                                                                                                 gCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGgtgt                        >  6:146843/1‑148 (MQ=255)
                                                                                                                     ccACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTa                      <  1:104560/146‑1 (MQ=255)
                                                                                                                     ccACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTAtt                    <  1:232106/148‑1 (MQ=255)
                                                                                                                     ccACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGAACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTa                      >  2:104560/1‑146 (MQ=255)
                                                                                                                      cACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACaa                                                     <  3:66711/114‑1 (MQ=255)
                                                                                                                      cACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACaa                                                     >  4:66711/1‑114 (MQ=255)
                                                                                                                       aCGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGc                 <  1:164298/149‑1 (MQ=255)
                                                                                                                            aCCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCAt            <  2:155499/149‑1 (MQ=255)
                                                                                                                             ccGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTgg                            <  2:232023/132‑1 (MQ=255)
                                                                                                                             ccGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTgg                            >  1:232023/1‑132 (MQ=255)
                                                                                                                              cGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCggccagg                                     >  2:12358/1‑122 (MQ=255)
                                                                                                                              cGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCggccagg                                     <  1:12358/122‑1 (MQ=255)
                                                                                                                                  cGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGGCGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATgg      <  2:353219/149‑1 (MQ=255)
                                                                                                                                      gcgcTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATggcggc  <  1:100505/149‑1 (MQ=255)
                                                                                                                                      gcgcTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGACGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATggcgg   >  6:29347/1‑148 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
TGACGCCCGGCGTCAGGGTCGCCGGCAAGGCAAGGCTGGCGGTCTCCAGCGAAGCCACGGGGTAAGCGCAGTAGTCGGCTGCGTAATAGGCACTGGCGCGATGGTTGCCACTGGCTCCCACGCCACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGC  >  NC_002947/5087354‑5087636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑