| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,092,577 | G→C | 10.3% | intergenic (‑273/+64) | astC ← / ← PP_4482 | acetylornithine aminotransferase/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,092,577 | 0 | G | C | 10.3% | 107.0 / 2.5 | 37 | intergenic (‑273/+64) | astC/PP_4482 | acetylornithine aminotransferase/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base C (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01 | |||||||||||
TCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCAC > NC_002947/5092436‑5092723 | tCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGgcggcg < 8:78452/148‑1 (MQ=255) aaTGCGACAAACACTGTCGCAATAGCGCAGTGTACGTGAGAGAGGTGGCTTGGTGAACGGGTGCTCACACATGATTGTGAGGGGGCAGGATGTACGTCGATGTTGATGTGTTGACTGGGAGATCGAGCGCCGCGTGGGCGGGGCTCGAt < 8:206352/149‑1 (MQ=255) cGACCAACCCTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGGGAACGGGGTCTCACCCAGGATTGTGAGGGGGCCGGATGTACGGCGATGTTGATGTGTTGACGGGCAAATCGAGCGCCGCCCACGCGGCGCTCGAtctcc < 6:218952/149‑5 (MQ=37) acTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAac < 8:329723/149‑1 (MQ=255) tGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAaaac > 3:38977/1‑149 (MQ=255) cgcAGGCTACGGGAGAGAGGGGGCGGGGGCAACGGGCGCCCACACAGGATCGGCCGGGGCCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAgg < 3:318809/149‑1 (MQ=255) ggggCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCGCGATTTGCGCCCCAACACCCACCCCAAGGCATACCCCCCGCacccccc > 6:244795/1‑149 (MQ=255) ctatctggTGCTCACATAGGTTTGTGAGTGGTCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCTAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCACAACAGCAACCCCAAGGCATACACCCATAACCCACGCGATAtct < 4:262062/142‑1 (MQ=255) gAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAAtct > 3:191548/1‑147 (MQ=255) ggTTCTCACACAGGATTGTGAGGGGGCAGGTTGTACGGCGATGTTGATGTGTTGACGGTGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCa < 3:72538/144‑1 (MQ=255) ggTGCTCACACAGGATTGTGAGGCGGCAGGAAGTACGGCGATGTTCATCTGATGACGGGGAGATCGAGAGCCGCCTGCGCGGCGCTCGATTTGCCCCCCAACACCAACCCCAAAGCATACACCCAGAACACACCCGAAATCTAAgaga > 4:72538/1‑145 (MQ=255) gTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACAACCCGAACCCACCCGAAAtct > 3:262062/1‑141 (MQ=255) gCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCttt > 8:176638/1‑148 (MQ=255) acactGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGc > 5:73205/1‑149 (MQ=255) cacaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAAtc < 4:138850/133‑1 (MQ=255) cacaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAACGCATACACCCCGAACCCACCCGAAAtc > 3:138850/1‑133 (MQ=255) ttGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGcc > 8:5848/1‑148 (MQ=255) tgtgAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCg < 4:191548/148‑1 (MQ=255) gAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCCCACGCGGCGCTc < 2:122844/68‑1 (MQ=37) gAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCCCACGCGGCGCTc > 1:122844/1‑68 (MQ=37) tGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACgg < 7:176638/149‑1 (MQ=255) ggCGATGTTGATGTGTTGACGGGGAGAGCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGgctgc < 6:17930/149‑1 (MQ=255) cGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAAc > 6:99622/1‑70 (MQ=255) cGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAAc < 5:99622/70‑1 (MQ=255) gATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGaa < 6:320576/99‑1 (MQ=255) gATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGaa > 5:320576/1‑99 (MQ=255) tGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGgctgctgc < 7:335767/144‑1 (MQ=255) tGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGgctgctgc > 8:335767/1‑144 (MQ=255) gtTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACCCCCCGCACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGa > 6:166721/1‑149 (MQ=255) gACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGAtt < 6:73205/148‑1 (MQ=255) aCGGGGAGATCGAGCGCCGCGGGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTg < 5:244795/148‑1 (MQ=255) cGAGCGCCGCGTGGGCGGCGTTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGACCCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCcg > 5:198387/1‑149 (MQ=255) cGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCcg > 4:194296/1‑149 (MQ=255) gccgcGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGgcg < 4:7588/92‑1 (MQ=255) gccgcGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGgcg > 3:7588/1‑92 (MQ=255) gcgTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTg > 2:6838/1‑149 (MQ=255) tGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCAc < 5:166721/149‑1 (MQ=255) | TCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCTGCTGCTGCGACGCTGATTGCGGTCTTCCCGCGGCGTTGCAC > NC_002947/5092436‑5092723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |