Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,116,500 T→G 6.0% L29R (CTG→CGG)  PP_4502 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,116,5000TG 6.0% 210.9 / 3.6 67L29R (CTG→CGG) PP_4502hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GCCTGGGCATGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCGAATT  >  NC_002947/5116352‑5116641
                                                                                                                                                    |                                                                                                                                             
gCCTGGGCATGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCt                                                                                                                                               >  4:333255/1‑149 (MQ=255)
      gCATGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGgccgc                                                                                                                                         <  3:333255/149‑1 (MQ=255)
        aTGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGccgccc                                                                                                                                       >  1:230217/1‑149 (MQ=255)
        aTGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGccgccc                                                                                                                                       <  2:230217/149‑1 (MQ=255)
            ccGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGGCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCgcccgccc                                                                                                                                   <  6:84594/149‑1 (MQ=255)
             cGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGcccgcccg                                                                                                                                  >  3:324953/1‑149 (MQ=255)
               tGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGgc                                                                                                                                >  5:30438/1‑149 (MQ=255)
                   cTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGgcgc                                                                                                                              <  4:15592/147‑1 (MQ=255)
                   cTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGgcgc                                                                                                                              >  3:15592/1‑147 (MQ=255)
                       cTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGcaca                                                                                                                           >  2:93943/1‑146 (MQ=255)
                       cTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGACAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGcaca                                                                                                                           <  1:93943/146‑1 (MQ=255)
                          gtggCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAAc                                                                                                                     >  4:299273/1‑149 (MQ=255)
                          gtggCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAAc                                                                                                                     >  5:212153/1‑149 (MQ=255)
                            ggCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGc                                                                                                                   >  8:59467/1‑149 (MQ=255)
                            ggCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGc                                                                                                                   <  6:30438/149‑1 (MQ=255)
                               gcAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTg                                                                                                                >  7:157510/1‑149 (MQ=255)
                                           ccTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaag                                                                                                    <  8:157510/149‑1 (MQ=255)
                                              gTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggcc                                                                                                 >  8:169898/1‑149 (MQ=255)
                                               tCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggccg                                                                                                <  5:226345/149‑1 (MQ=255)
                                               tCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggcc                                                                                                 >  3:276366/1‑148 (MQ=255)
                                                 gcgcCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggccg                                                                                                >  4:253061/1‑147 (MQ=255)
                                                        cTGCACGATGACCCTTCTACCTCCAAACACAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCcgcg                                                                                       >  1:16444/1‑149 (MQ=255)
                                                         tGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgt                                                                                      >  2:45243/1‑149 (MQ=255)
                                                         tGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgt                                                                                      >  1:246582/1‑149 (MQ=255)
                                                          gCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgtg                                                                                     >  5:277028/1‑149 (MQ=255)
                                                           cACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgtgg                                                                                    >  3:256103/1‑149 (MQ=255)
                                                           cACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgtg                                                                                     <  2:16444/148‑1 (MQ=255)
                                                              gATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCgg                                                                                                                                 >  6:192962/1‑101 (MQ=255)
                                                              gATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCgg                                                                                                                                 <  5:192962/101‑1 (MQ=255)
                                                                  aCCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTggc                                                                             <  1:45243/149‑1 (MQ=255)
                                                                        cTACCTCCACACCCAACGATTGACATGCTCTACGCCATTATCGCCCGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTggccggc                                                                         <  4:324953/147‑1 (MQ=255)
                                                                             tCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGc                                                                     <  8:101458/146‑1 (MQ=255)
                                                                             tCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGc                                                                     >  7:101458/1‑146 (MQ=255)
                                                                                aaaCCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCgg                                                               <  3:253061/149‑1 (MQ=255)
                                                                                        ggATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGAc                                                       <  6:212153/149‑1 (MQ=255)
                                                                                         gATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACa                                                      <  2:246582/149‑1 (MQ=255)
                                                                                           ttGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGc                                                    <  4:62923/149‑1 (MQ=255)
                                                                                              aCATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAAc                                                 >  5:263570/1‑149 (MQ=255)
                                                                                                 tGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGAc                                              >  4:134120/1‑149 (MQ=255)
                                                                                                        cGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgtggt                                                                                   <  1:235651/105‑1 (MQ=255)
                                                                                                        cGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCgtggt                                                                                   >  2:235651/1‑105 (MQ=255)
                                                                                                            aTTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGc                                   <  3:299273/149‑1 (MQ=255)
                                                                                                              tATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCgg                                 >  5:4435/1‑149 (MQ=255)
                                                                                                               aTCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCgg                                 <  6:263570/148‑1 (MQ=255)
                                                                                                               aTCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCgg                                 <  4:276366/148‑1 (MQ=255)
                                                                                                                 cGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCgg                                 >  6:258384/1‑146 (MQ=255)
                                                                                                                 cGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCgg                                 <  5:258384/146‑1 (MQ=255)
                                                                                                                        gACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCgg                       <  7:59467/149‑1 (MQ=255)
                                                                                                                          cGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTa                     <  6:277028/149‑1 (MQ=255)
                                                                                                                            tCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGc                   >  6:284547/1‑149 (MQ=255)
                                                                                                                               cAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggc                                                                                                  <  5:281051/67‑1 (MQ=255)
                                                                                                                               cAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCaaggc                                                                                                  >  6:281051/1‑67 (MQ=255)
                                                                                                                               cAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCt                 >  5:91020/1‑148 (MQ=255)
                                                                                                                                    tCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGt           >  2:168658/1‑149 (MQ=255)
                                                                                                                                      ccTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTggcc                                                                            >  7:157866/1‑82 (MQ=255)
                                                                                                                                      ccTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTggcc                                                                            <  8:157866/82‑1 (MQ=255)
                                                                                                                                       cTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGc                                                              <  7:4380/95‑1 (MQ=255)
                                                                                                                                       cTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGc                                                              >  8:4380/1‑95 (MQ=255)
                                                                                                                                         ggAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGcc       >  7:16090/1‑148 (MQ=255)
                                                                                                                                         ggAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGcc       >  7:295323/1‑148 (MQ=255)
                                                                                                                                          gAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGACGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCg      >  8:299768/1‑148 (MQ=255)
                                                                                                                                             aaGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCGAAtt  <  4:256103/149‑1 (MQ=255)
                                                                                                                                              tgcgcCGGGCGGGCGGCCAGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGc        >  6:180696/2‑142 (MQ=255)
                                                                                                                                              tgcgcCGGGCGGGCGGCCAGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGc        <  5:180696/141‑1 (MQ=255)
                                                                                                                                               gcgcCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGc                                                    >  1:89453/1‑97 (MQ=255)
                                                                                                                                               gcgcCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGc                                                    <  2:89453/97‑1 (MQ=255)
                                                                                                                                               gcgcCGGGCGGGCGGCCAGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCGAAtt  <  2:194764/147‑1 (MQ=255)
                                                                                                                                               gcgcCGGGCGGGCGGCCAGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCGAAtt  >  1:194764/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCCTGGGCATGACCGTGATCTTCCTGGTGGCGCAAGGCGTGTACCTGTCGCGCCACCTGCACGATGACCCTTCTACCTCCAAACCCAAGGATTGACATGCTCTACGCCATTATCGCCAGCGACGTCGCAAACTCCCTGGAAAAGCGCCTGGCCGCCCGCCCGGCGCACATCGAACGCCTGCAGCAGCTCAAGGCCGAAGGCCGCGTGGTGTTGGCCGGCCCGCACCCGGCCATCGACAGCAACGACCCGGGCGAAGCGGGTTTCAGCGGTAGCCTGATCGTTGCCGAATT  >  NC_002947/5116352‑5116641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑