Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,120,362 C→T 8.5% A2V (GCG→GTG)  trkH‑II → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,120,3620CT 8.5% 131.7 / 2.5 48A2V (GCG→GTG) trkH‑IIpotassium transporter
Reads supporting (aligned to +/- strand):  ref base C (19/24);  new base T (2/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CACGGTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCTT  >  NC_002947/5120225‑5120509
                                                                                                                                         |                                                                                                                                                   
cacGGTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGAcat                                                                                                                                          >  2:211504/1‑147 (MQ=255)
   ggTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCtt                                                                                                                                         >  7:121346/1‑147 (MQ=255)
    gTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                      >  3:297256/1‑149 (MQ=255)
              gCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGt                                                                                                                            >  5:327034/1‑149 (MQ=255)
                 tCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTtc                                                                                                                         <  8:121346/149‑1 (MQ=255)
                 tCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTtc                                                                                                                         <  5:82458/149‑1 (MQ=255)
                         cTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCgg                                                                                                                 >  6:3907/1‑149 (MQ=255)
                            cATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGAcc                                                                                                                                           >  1:248028/1‑120 (MQ=255)
                            cATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGAcc                                                                                                                                           <  2:248028/120‑1 (MQ=255)
                             aTCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATGCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATc                                                                                                             >  7:215182/1‑149 (MQ=255)
                             aTCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATGCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATc                                                                                                             >  7:209628/1‑149 (MQ=255)
                                ggCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                      >  6:334703/1‑121 (MQ=255)
                                ggCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                      <  5:334703/121‑1 (MQ=255)
                                  cTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCt                                                                                                        <  5:3907/149‑1 (MQ=255)
                                      cctcctGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGAt                                                                                                     >  2:63163/1‑148 (MQ=255)
                                             gCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTg                                                                                              <  2:92762/148‑1 (MQ=255)
                                                ggTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCg                                                                                          >  2:340651/1‑149 (MQ=255)
                                                   gAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGt                                                                                         >  4:232539/1‑147 (MQ=255)
                                                    aaTGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                       <  4:297256/148‑1 (MQ=255)
                                                        tgCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCAt                                                                                   <  3:232539/148‑1 (MQ=255)
                                                           ggCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGcc                                                                               >  7:79120/1‑149 (MQ=255)
                                                              cATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTg                                                                            <  8:79120/149‑1 (MQ=255)
                                                              cATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCATGCTCACGGccagc                                                                            <  6:40961/149‑4 (MQ=255)
                                                                ttCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGcc                                                                          <  6:327034/149‑1 (MQ=255)
                                                                             aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                       <  7:329174/123‑1 (MQ=255)
                                                                             aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                       >  8:329174/1‑123 (MQ=255)
                                                                             aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                             >  7:34123/1‑149 (MQ=255)
                                                                             aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                             <  1:340651/149‑1 (MQ=255)
                                                                                aCCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTg                                                          <  8:34123/149‑1 (MQ=255)
                                                                                aCCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATAGGTTTCATCATCGGCATCTTCCTCATTACGCTGGCCGAGAGCAAGGCCGTGCCCATGGCGACCCtccg                                                           >  5:234348/1‑145 (MQ=255)
                                                                                        cATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                  <  8:215182/149‑1 (MQ=255)
                                                                                        cATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                  <  8:209628/149‑1 (MQ=255)
                                                                                         aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGTCGTTGCCGACCTAAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGACGTGAGCATGGACGTTCGCATGGCGACCCTGGTGATCTTCGAg                                                 >  8:328266/1‑149 (MQ=255)
                                                                                         aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                             <  8:127460/137‑1 (MQ=255)
                                                                                         aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                             >  7:127460/1‑137 (MQ=255)
                                                                                             gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATg                                                                      >  6:209043/1‑124 (MQ=255)
                                                                                             gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATg                                                                      <  5:209043/124‑1 (MQ=255)
                                                                                             gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCa                                             <  1:63163/149‑1 (MQ=255)
                                                                                                gAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGTCATCTATCTGATTAGGCTGGCCGTGAGCTTGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCAcct                                          <  5:250430/149‑2 (MQ=255)
                                                                                                     gcgcCCTTCCGTTTCGAGGCTGAGCAGAGCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGa                                      <  3:65578/148‑1 (MQ=255)
                                                                                                         ccTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTg                                                                                                                              <  1:38854/56‑1 (MQ=255)
                                                                                                         ccTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTg                                                                                                                              >  2:38854/1‑56 (MQ=255)
                                                                                                                         tGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                  <  2:304419/116‑1 (MQ=255)
                                                                                                                             cAGATCACATGGGGTTGCCGAGCTTCAGGCTCATTGGGTTCATCATCGGCATCTTCCTGTTTTCGCTGGCCGGGAGCATGGCCGGGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGc             <  6:234348/149‑1 (MQ=255)
                                                                                                                                    caTGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGTTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCa      <  7:328266/149‑1 (MQ=255)
                                                                                                                                    caTGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCa      <  1:211504/149‑1 (MQ=255)
                                                                                                                                        gCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGa         >  6:230391/1‑142 (MQ=255)
                                                                                                                                        gCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGa         <  5:230391/142‑1 (MQ=255)
                                                                                                                                         cGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCtt  >  2:188824/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CACGGTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCTT  >  NC_002947/5120225‑5120509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑