Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,120,373 T→C 8.7% L6L (TTA→CTA)  trkH‑II → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,120,3730TC 8.7% 122.4 / 2.7 46L6L (TTA→CTA) trkH‑IIpotassium transporter
Reads supporting (aligned to +/- strand):  ref base T (18/24);  new base C (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GGTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCTTCATCGCC  >  NC_002947/5120228‑5120516
                                                                                                                                                 |                                                                                                                                               
ggTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCtt                                                                                                                                                >  7:121346/1‑147 (MQ=255)
 gTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                             >  3:297256/1‑149 (MQ=255)
           gCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGt                                                                                                                                   >  5:327034/1‑149 (MQ=255)
              tCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTtc                                                                                                                                <  5:82458/149‑1 (MQ=255)
              tCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTtc                                                                                                                                <  8:121346/149‑1 (MQ=255)
                      cTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCgg                                                                                                                        >  6:3907/1‑149 (MQ=255)
                          aTCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATGCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATc                                                                                                                    >  7:209628/1‑149 (MQ=255)
                          aTCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATGCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATc                                                                                                                    >  7:215182/1‑149 (MQ=255)
                             ggCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                             >  6:334703/1‑121 (MQ=255)
                             ggCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAg                                                                                                                                             <  5:334703/121‑1 (MQ=255)
                               cTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCt                                                                                                               <  5:3907/149‑1 (MQ=255)
                                   cctcctGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGAt                                                                                                            >  2:63163/1‑148 (MQ=255)
                                          gCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTg                                                                                                     <  2:92762/148‑1 (MQ=255)
                                             ggTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCg                                                                                                 >  2:340651/1‑149 (MQ=255)
                                                gAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGt                                                                                                >  4:232539/1‑147 (MQ=255)
                                                 aaTGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                              <  4:297256/148‑1 (MQ=255)
                                                     tgCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCAt                                                                                          <  3:232539/148‑1 (MQ=255)
                                                        ggCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGcc                                                                                      >  7:79120/1‑149 (MQ=255)
                                                           cATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTg                                                                                   <  8:79120/149‑1 (MQ=255)
                                                           cATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCATGCTCACGGccagc                                                                                   <  6:40961/149‑4 (MQ=255)
                                                             ttCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGcc                                                                                 <  6:327034/149‑1 (MQ=255)
                                                                          aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                              <  7:329174/123‑1 (MQ=255)
                                                                          aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGa                                                                                              >  8:329174/1‑123 (MQ=255)
                                                                          aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                                    >  7:34123/1‑149 (MQ=255)
                                                                          aTCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                                    <  1:340651/149‑1 (MQ=255)
                                                                             aCCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTg                                                                 <  8:34123/149‑1 (MQ=255)
                                                                             aCCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATAGGTTTCATCATCGGCATCTTCCTCATTACGCTGGCCGAGAGCAAGGCCGTGCCCATGGCGACCCtccg                                                                  >  5:234348/1‑145 (MQ=255)
                                                                                     cATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                         <  8:215182/149‑1 (MQ=255)
                                                                                     cATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGTGTTGCCGACCCTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                         <  8:209628/149‑1 (MQ=255)
                                                                                      aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGTCGTTGCCGACCTAAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGACGTGAGCATGGACGTTCGCATGGCGACCCTGGTGATCTTCGAg                                                        >  8:328266/1‑149 (MQ=255)
                                                                                      aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                                    >  7:127460/1‑137 (MQ=255)
                                                                                      aTAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTg                                                                    <  8:127460/137‑1 (MQ=255)
                                                                                          gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATg                                                                             >  6:209043/1‑124 (MQ=255)
                                                                                          gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATg                                                                             <  5:209043/124‑1 (MQ=255)
                                                                                          gTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCa                                                    <  1:63163/149‑1 (MQ=255)
                                                                                             gAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGTCATCTATCTGATTAGGCTGGCCGTGAGCTTGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCAcct                                                 <  5:250430/149‑2 (MQ=255)
                                                                                                  gcgcCCTTCCGTTTCGAGGCTGAGCAGAGCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGa                                             <  3:65578/148‑1 (MQ=255)
                                                                                                      ccTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTg                                                                                                                                     >  2:38854/1‑56 (MQ=255)
                                                                                                      ccTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTg                                                                                                                                     <  1:38854/56‑1 (MQ=255)
                                                                                                                      tGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGa                                                         <  2:304419/116‑1 (MQ=255)
                                                                                                                          cAGATCACATGGGGTTGCCGAGCTTCAGGCTCATTGGGTTCATCATCGGCATCTTCCTGTTTTCGCTGGCCGGGAGCATGGCCGGGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGc                    <  6:234348/149‑1 (MQ=255)
                                                                                                                                 caTGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGTTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCa             <  7:328266/149‑1 (MQ=255)
                                                                                                                                 caTGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCa             <  1:211504/149‑1 (MQ=255)
                                                                                                                                     gCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGa                <  5:230391/142‑1 (MQ=255)
                                                                                                                                     gCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGa                >  6:230391/1‑142 (MQ=255)
                                                                                                                                      cGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCtt         >  2:188824/1‑148 (MQ=255)
                                                                                                                                            cGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCTTCATCGcc  >  6:340483/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGTTGAGCAATGCGTCGAGAGCCTCCATCGGCTACCCTCCTGGCAGGTGAATGTGCGGCCATTCTAGCGTTTACATCACCAGACCCATAGGTAGAATGGCGCCCTTCCGTTTCGAGTCTGAGCAGATCACATGGCGTTGCCGACCTTAAGGATCATTGGTTTCATCATCGGCATCTTCCTGATTACGCTGGCCGTGAGCATGGCCGTGCCCATGGCGACCCTGGTGATCTTCGAGCGCACCGGTGACATGCCGTCGTTTCTCTGGTCGAGCCTGATCACCTTCATCGCC  >  NC_002947/5120228‑5120516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑