Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,160,639 G→C 11.3% P139R (CCA→CGA) ‡ PP_4543 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,160,6390GC11.3% 177.1 / 12.1 62P139R (CCA→CGA) ‡PP_4543hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/30);  new base C (3/4);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

ATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCGGG  >  NC_002947/5160492‑5160772
                                                                                                                                                    |                                                                                                                                     
atccaCCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTgg                                                                                                                                      <  3:182293/149‑1 (MQ=255)
 tccaCCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGCGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTgg                                                                                                                                      <  1:89733/149‑1 (MQ=255)
   caCCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGc                                                                                                                                    <  8:202698/148‑1 (MQ=255)
    aCCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCg                                                                                                                                  >  3:124241/1‑149 (MQ=255)
       caggcaggCCCGCCAACTGCGCGTCCAGCGCAGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTg                                                                                                                               >  1:219228/1‑149 (MQ=255)
          gcaggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCGGCCCACGGCTGCTGAcg                                                                                                                            <  3:351970/149‑2 (MQ=255)
          gcaggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGAcc                                                                                                                            >  8:36304/1‑149 (MQ=255)
           caggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCGGCCCACGGCTGCTGAcgg                                                                                                                           <  6:164740/149‑3 (MQ=255)
           caggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCg                                                                                                                           <  1:220149/149‑1 (MQ=255)
            aggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          <  6:330656/149‑1 (MQ=255)
             ggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCggg                                                                                                                         >  4:7567/1‑149 (MQ=255)
              gcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGc                                                                                                                        >  7:10766/1‑149 (MQ=255)
                ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          >  8:262665/1‑145 (MQ=255)
                ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          <  7:262665/145‑1 (MQ=255)
                ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       >  5:103019/1‑148 (MQ=255)
                 cGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTg                                                                                                                      >  7:263894/1‑148 (MQ=255)
                   ccAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAAc                                                                                                                   >  4:83907/1‑149 (MQ=255)
                   ccAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAAc                                                                                                                   <  4:124241/149‑1 (MQ=255)
                      aCTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCTGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGATGACCGGGCTGAACGGc                                                                                                                <  8:39045/149‑1 (MQ=255)
                                cAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       >  2:326886/1‑132 (MQ=255)
                                cAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       <  1:326886/132‑1 (MQ=255)
                                 aGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAg                                                                                                     <  6:258275/149‑1 (MQ=255)
                                        ggTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGtttt                                                                                              <  6:103019/149‑1 (MQ=255)
                                          tCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  2:219228/149‑1 (MQ=255)
                                          gcGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGTATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  8:72008/148‑1 (MQ=255)
                                             gTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgta                                                                                         <  3:7567/149‑1 (MQ=255)
                                             gTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgta                                                                                         <  1:241358/149‑1 (MQ=255)
                                                aaGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       <  6:32534/148‑1 (MQ=255)
                                                   ttGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGc                                                                                   >  8:301017/1‑149 (MQ=255)
                                                    tGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGc                                                                                   <  3:83907/148‑1 (MQ=255)
                                                      aTGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTggg                                                                                                                                     <  3:17666/96‑1 (MQ=255)
                                                      aTGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTggg                                                                                                                                     >  4:17666/1‑96 (MQ=255)
                                                      aTGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGa                                                                                 <  7:36304/148‑1 (MQ=255)
                                                              tCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCTGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCCGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGccc                                                                        >  1:248735/1‑149 (MQ=255)
                                                                 ggAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCt                                                                     >  4:165556/1‑149 (MQ=255)
                                                                    aaGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTca                                                                   >  5:10580/1‑148 (MQ=255)
                                                                      gCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACaa                                                                <  8:10766/149‑1 (MQ=255)
                                                                           cccTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            >  4:221041/1‑116 (MQ=255)
                                                                           cccTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  3:221041/116‑1 (MQ=255)
                                                                                  ccAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCaa                                                    <  6:10580/149‑1 (MQ=255)
                                                                                   cAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAAc                                                   <  7:301017/149‑1 (MQ=255)
                                                                                          tCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATc                                            >  1:179224/1‑149 (MQ=255)
                                                                                          tCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATc                                            >  3:323821/1‑149 (MQ=255)
                                                                                               cAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAaca                                       <  8:263894/149‑1 (MQ=255)
                                                                                                aGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       <  5:3305/100‑1 (MQ=255)
                                                                                                aGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       >  6:3305/1‑100 (MQ=255)
                                                                                                  g‑gCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCATCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACAt                                    <  6:51428/149‑1 (MQ=255)
                                                                                                  g‑gCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACAt                                    <  6:56828/149‑1 (MQ=255)
                                                                                                             aTCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTc                                >  7:280936/1‑143 (MQ=255)
                                                                                                             aTCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTc                                <  8:280936/143‑1 (MQ=255)
                                                                                                                 acaaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAg                      <  2:155568/149‑1 (MQ=255)
                                                                                                                  caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATc                                                           >  2:282912/1‑111 (MQ=255)
                                                                                                                  caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATc                                                           <  1:282912/111‑1 (MQ=255)
                                                                                                                  caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGt                     <  2:179224/149‑1 (MQ=255)
                                                                                                                   aaaCCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         >  7:223051/1‑128 (MQ=255)
                                                                                                                        gTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACt               >  7:41325/1‑149 (MQ=255)
                                                                                                                                   gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAAc                         >  2:57517/6‑128 (MQ=255)
                                                                                                                                   gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAAc                         <  1:57517/123‑1 (MQ=255)
                                                                                                                                   gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTAcgcg    >  7:292397/6‑149 (MQ=255)
                                                                                                                                    ggCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCgg   <  2:248735/149‑1 (MQ=255)
                                                                                                                                     gCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCggg  >  3:284444/1‑149 (MQ=255)
                                                                                                                                     gCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCggg  >  7:109558/1‑149 (MQ=255)
                                                                                                                                      cggTCAGCGGCCCACGGCTGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGAt                                             <  1:90406/103‑1 (MQ=255)
                                                                                                                                      cggTCAGCGGCCCACGGCTGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGAt                                             >  2:90406/3‑105 (MQ=255)
                                                                                                                                      ccGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         <  6:307984/109‑1 (MQ=255)
                                                                                                                                      ccGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         >  5:307984/1‑109 (MQ=255)
                                                                                                                                                    |                                                                                                                                     
ATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGG‑GCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCGGG  >  NC_002947/5160492‑5160772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑