Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,160,643	C→T	11.4%	G138S (GGC→AGC)	PP_4543 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,160,643	0	C	T	11.4%	162.5 / 9.2	61	G138S (GGC→AGC)	PP_4543	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/29);  new base T (3/4);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

ACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCGGG  >  NC_002947/5160496‑5160772                                                                                                                                                    |                                                                                                                                  aCCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCg                                                                                                                                  >  3:124241/1‑149 (MQ=255)    caggcaggCCCGCCAACTGCGCGTCCAGCGCAGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTg                                                                                                                               >  1:219228/1‑149 (MQ=255)       gcaggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCGGCCCACGGCTGCTGAcg                                                                                                                            <  3:351970/149‑2 (MQ=255)       gcaggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGAcc                                                                                                                            >  8:36304/1‑149 (MQ=255)        caggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCGGCCCACGGCTGCTGAcgg                                                                                                                           <  6:164740/149‑3 (MQ=255)        caggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCg                                                                                                                           <  1:220149/149‑1 (MQ=255)         aggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          <  6:330656/149‑1 (MQ=255)          ggcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCggg                                                                                                                         >  4:7567/1‑149 (MQ=255)           gcCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGc                                                                                                                        >  7:10766/1‑149 (MQ=255)             ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          >  8:262665/1‑145 (MQ=255)             ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCgg                                                                                                                          <  7:262665/145‑1 (MQ=255)             ccGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       >  5:103019/1‑148 (MQ=255)              cGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTg                                                                                                                      >  7:263894/1‑148 (MQ=255)                ccAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAAc                                                                                                                   <  4:124241/149‑1 (MQ=255)                ccAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAAc                                                                                                                   >  4:83907/1‑149 (MQ=255)                   aCTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCTGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGATGACCGGGCTGAACGGc                                                                                                                <  8:39045/149‑1 (MQ=255)                             cAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       <  1:326886/132‑1 (MQ=255)                             cAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCt                                                                                                                       >  2:326886/1‑132 (MQ=255)                              aGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAg                                                                                                     <  6:258275/149‑1 (MQ=255)                                     ggTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGtttt                                                                                              <  6:103019/149‑1 (MQ=255)                                       tCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  2:219228/149‑1 (MQ=255)                                       gcGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGTATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  8:72008/148‑1 (MQ=255)                                          gTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgta                                                                                         <  3:7567/149‑1 (MQ=255)                                          gTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgta                                                                                         <  1:241358/149‑1 (MQ=255)                                             aaGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       <  6:32534/148‑1 (MQ=255)                                                ttGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGc                                                                                   >  8:301017/1‑149 (MQ=255)                                                 tGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGc                                                                                   <  3:83907/148‑1 (MQ=255)                                                   aTGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGa                                                                                 <  7:36304/148‑1 (MQ=255)                                                           tCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCTGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCCGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGccc                                                                        >  1:248735/1‑149 (MQ=255)                                                              ggAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCt                                                                     >  4:165556/1‑149 (MQ=255)                                                                 aaGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTca                                                                   >  5:10580/1‑148 (MQ=255)                                                                   gCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACaa                                                                <  8:10766/149‑1 (MQ=255)                                                                        cccTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            >  4:221041/1‑116 (MQ=255)                                                                        cccTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTgg                                                                                            <  3:221041/116‑1 (MQ=255)                                                                               ccAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCaa                                                    <  6:10580/149‑1 (MQ=255)                                                                                cAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAAc                                                   <  7:301017/149‑1 (MQ=255)                                                                                       tCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATc                                            >  1:179224/1‑149 (MQ=255)                                                                                       tCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATc                                            >  3:323821/1‑149 (MQ=255)                                                                                            cAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAaca                                       <  8:263894/149‑1 (MQ=255)                                                                                             aGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       >  6:3305/1‑100 (MQ=255)                                                                                             aGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGgtagt                                                                                       <  5:3305/100‑1 (MQ=255)                                                                                               ggCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCATCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACAt                                    <  6:51428/149‑1 (MQ=255)                                                                                               ggCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACAt                                    <  6:56828/149‑1 (MQ=255)                                                                                                         aTCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTc                                <  8:280936/143‑1 (MQ=255)                                                                                                         aTCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTc                                >  7:280936/1‑143 (MQ=255)                                                                                                             acaaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAg                      <  2:155568/149‑1 (MQ=255)                                                                                                              caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATc                                                           >  2:282912/1‑111 (MQ=255)                                                                                                              caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATc                                                           <  1:282912/111‑1 (MQ=255)                                                                                                              caaACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGt                     <  2:179224/149‑1 (MQ=255)                                                                                                               aaaCCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         >  7:223051/1‑128 (MQ=255)                                                                                                                    gTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACt               >  7:41325/1‑149 (MQ=255)                                                                                                                               gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAAc                         <  1:57517/123‑1 (MQ=255)                                                                                                                               gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAAc                         >  2:57517/6‑128 (MQ=255)                                                                                                                               gcccggTCAGCGGCCCACGGCTGCTGACGGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTAcgcg    >  7:292397/6‑149 (MQ=255)                                                                                                                                ggCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCgg   <  2:248735/149‑1 (MQ=255)                                                                                                                                 gCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCggg  >  7:109558/1‑149 (MQ=255)                                                                                                                                 gCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCggg  >  3:284444/1‑149 (MQ=255)                                                                                                                                  cggTCAGCGGCCCACGGCTGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGAt                                             <  1:90406/103‑1 (MQ=255)                                                                                                                                  cggTCAGCGGCCCACGGCTGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGAt                                             >  2:90406/3‑105 (MQ=255)                                                                                                                                  ccGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         <  6:307984/109‑1 (MQ=255)                                                                                                                                  ccGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGaa                                         >  5:307984/1‑109 (MQ=255)                                                                                                                                                    cGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCgg   >  7:333791/1‑129 (MQ=255)                                                                                                                                                    cGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCgg   <  8:333791/129‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                  ACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATACTCCGGCAGGGCCTGCAGCATCAACAAACCGTCAGCCGGATCGGCCGTCAGCAGCCGTGGGCCGCTGACCGGGCTGAACGGCACAGCGCCAAGCAGTTTTGGGTAGTAGGCGATACCGGCCCGCTCACAAGCATCGGCCCAACCATGATCGAACACATACTCGCCAAACGAGTGCCACTTGCGGTACGCGGG  >  NC_002947/5160496‑5160772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑