Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,173,248 G→C 10.0% intergenic (‑289/+43) fadD‑I ← / ← fadD‑II long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,173,2480GC10.0% 138.9 / 4.9 49intergenic (‑289/+43)fadD‑I/fadD‑IIlong‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base C (3/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTT  >  NC_002947/5173100‑5173395
                                                                                                                                                    |                                                                                                                                                   
gCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTggg                                                                                                                                                     <  1:271075/149‑1 (MQ=255)
          gCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAg                                                                                                                                             >  5:178607/1‑147 (MQ=255)
               aTGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCATGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCTCTCCTACATTGGGCAGTGTAGGAGCcgg                                                                                                                                      >  6:206300/1‑149 (MQ=255)
                   tCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTa                                                                                                                                  >  7:92396/1‑149 (MQ=255)
                     acaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCa                                                                                                                                                   <  4:138569/130‑1 (MQ=255)
                     acaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCa                                                                                                                                                   >  3:138569/1‑130 (MQ=255)
                     acaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTAc                                                                                                                                 <  6:333278/148‑1 (MQ=255)
                       aaaaaTGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAgt                                                                                                                                                 <  4:35469/130‑1 (MQ=255)
                       aaaaaTGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAgt                                                                                                                                                 >  3:35469/1‑130 (MQ=255)
                       aaaaaTGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCcg                                                                                                                              >  4:63847/1‑149 (MQ=255)
                              aCTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCgaaga                                                                                                                        <  6:178607/148‑1 (MQ=255)
                               atCAATGAACAATACAGTTCATTTTTAGAGTAATTATCCAAAATGTCGCCCATAAGGCGGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCgaagaa                                                                                                                       <  8:338451/147‑1 (MQ=255)
                                tCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTTAACCCGCTGCGACAATGGGGAGTGTTGGAGCGGGGTTAACCGCgtagaacc                                                                                                                     >  4:17863/1‑147 (MQ=255)
                                tCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGc                                                                                                                     >  7:68915/1‑149 (MQ=255)
                                          aTACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTa                                                                                                           <  3:63847/149‑1 (MQ=255)
                                                          gagTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTCGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg                                                                                            <  5:230/148‑1 (MQ=255)
                                                            gTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCaa                                                                                         >  7:7143/1‑149 (MQ=255)
                                                                               cAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg                                                                      <  8:7143/149‑1 (MQ=255)
                                                                                aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg                                                                                            <  3:33858/126‑1 (MQ=255)
                                                                                aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg                                                                                            >  4:33858/1‑126 (MQ=255)
                                                                                       gCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCgg                                                              >  1:270447/1‑149 (MQ=255)
                                                                                        cTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGc                                                             <  8:92396/149‑1 (MQ=255)
                                                                                              agagCCTGTTTGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACAATGGGCAGGGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATg                                                       <  5:206300/149‑1 (MQ=255)
                                                                                               gagCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg                                                      <  8:68915/149‑1 (MQ=255)
                                                                                                agCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATggg                                                     <  2:270447/149‑1 (MQ=255)
                                                                                                 gCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTAc                                                                                                                                 >  6:62325/1‑72 (MQ=255)
                                                                                                 gCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTAc                                                                                                                                 <  5:62325/72‑1 (MQ=255)
                                                                                                  ccTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg                                                                      >  2:30676/1‑130 (MQ=255)
                                                                                                  ccTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGc                                                    >  6:123148/1‑148 (MQ=255)
                                                                                                  ccTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCAATATCGCGCAACTCCCGCCGCAAAATCTTg                                                                      <  1:30676/130‑1 (MQ=255)
                                                                                                    tGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAAc                                                 >  7:149716/1‑149 (MQ=255)
                                                                                                     gTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAAc                                                 >  3:222330/1‑148 (MQ=255)
                                                                                                         gagCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTTGGGAGTGTAGGAGCGGGGTTACCCGCGAAGAAGCCATCCCGGGATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCCCCGGCGGGATGGGCAACCATTc                                            >  2:301234/1‑149 (MQ=38)
                                                                                                           gCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCAc                                                                 <  2:242609/126‑1 (MQ=255)
                                                                                                           gCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCAc                                                                 >  1:242609/1‑126 (MQ=255)
                                                                                                              gTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCgca                                       <  8:149716/149‑1 (MQ=255)
                                                                                                                      cATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAtg                               <  5:131364/149‑1 (MQ=255)
                                                                                                                                  aaCCCGCTCCTACATGCTCCAGTGTCGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACt                   <  1:301234/149‑1 (MQ=255)
                                                                                                                                    cccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACttt                 >  8:293107/1‑149 (MQ=255)
                                                                                                                                    cccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg                        <  1:314037/142‑1 (MQ=255)
                                                                                                                                    cccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg                        >  2:314037/1‑142 (MQ=255)
                                                                                                                                     ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTAACACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCCCAGCACGATGTCCTTGGGTACTTTg                >  3:252109/1‑149 (MQ=255)
                                                                                                                                      cGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCGATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCCGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGt               >  4:288730/1‑149 (MQ=38)
                                                                                                                                       gCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTa              <  4:222330/149‑1 (MQ=255)
                                                                                                                                           cTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCAt                                                        <  6:137851/103‑1 (MQ=255)
                                                                                                                                           cTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCAt                                                        >  5:137851/1‑103 (MQ=255)
                                                                                                                                           cTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCg          >  4:116542/1‑149 (MQ=255)
                                                                                                                                               aTTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTg       >  6:144859/1‑148 (MQ=255)
                                                                                                                                               aTTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTg       >  3:142419/1‑148 (MQ=255)
                                                                                                                                                ttGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGaa     >  3:267531/1‑149 (MQ=255)
                                                                                                                                                   ggCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGtt  <  4:267531/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTT  >  NC_002947/5173100‑5173395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑