Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,757 C→G 8.3% Q83E (CAA→GAA)  PP_4562 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,7570CG 8.3% 180.3 / 6.5 61Q83E (CAA→GAA) PP_4562hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base G (2/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

ACGCAACCTGTGGTGGTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTT  >  NC_002947/5182612‑5182905
                                                                                                                                                 |                                                                                                                                                    
acgcccccTGTGGTGGTTCGCAGGGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGTGAGCCTTGAGGaaa                                                                                                                                                   <  8:238260/143‑1 (MQ=255)
 cGCAACCTGTGGTGGTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAAt                                                                                                                                                  <  4:266762/149‑1 (MQ=255)
      ccTGTGGTGGTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGTGAGCCTTGAGGaaa                                                                                                                                                   >  1:179416/1‑143 (MQ=255)
      ccTGTGGTGGTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGTGAGCCTTGAGGaaa                                                                                                                                                   <  2:179416/143‑1 (MQ=255)
          tggtggTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTgcg                                                                                                                                         <  4:172926/149‑1 (MQ=255)
          tggtggTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTg                                                                                                                                           <  5:131757/147‑1 (MQ=255)
                  cGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTg                                                                                                                                 >  5:108687/1‑149 (MQ=255)
                     aGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGc                                                                                                                               >  1:149780/1‑148 (MQ=255)
                       tGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGTGAGCCTTGAGGaaa                                                                                                                                                   >  4:134550/1‑126 (MQ=255)
                       tGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGTGAGCCTTGAGGaaa                                                                                                                                                   <  3:134550/126‑1 (MQ=255)
                            gTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGcgccg                                                                                                                       >  3:138606/1‑149 (MQ=255)
                             tGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGcgccgc                                                                                                                      <  1:178336/149‑1 (MQ=255)
                                  ctgctgGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTccac                                                                                                                 <  8:144107/149‑1 (MQ=255)
                                   tgctgGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTccac                                                                                                                 >  5:357662/1‑148 (MQ=255)
                                     ctgGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGCTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGACTGGCAAGCGCCGCTccacca                                                                                                               >  8:1508/1‑148 (MQ=255)
                                        ggCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCa                                                                                                            <  4:138606/148‑1 (MQ=255)
                                         gCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGTAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGc                                                                                                          >  1:344296/1‑149 (MQ=255)
                                          cAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGcc                                                                                                         >  4:53713/1‑149 (MQ=255)
                                                ccAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCt                                                                                                        <  5:364165/144‑1 (MQ=255)
                                                ccAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCt                                                                                                        >  6:364165/1‑144 (MQ=255)
                                                ccAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGccc                                                                                                   >  4:285064/1‑149 (MQ=255)
                                                       gACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAAt                                                                                                                                                  <  5:208021/95‑1 (MQ=255)
                                                       gACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAAt                                                                                                                                                  >  6:208021/1‑95 (MQ=255)
                                                         cTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCg                                                                                          <  3:285064/149‑1 (MQ=255)
                                                              tCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAGGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCgg                                                                                     >  3:114772/1‑149 (MQ=255)
                                                              tCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCgg                                                                                     >  4:33877/1‑149 (MQ=255)
                                                                aaCCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGa                                                                                   <  3:33877/149‑1 (MQ=255)
                                                                 aCCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGAc                                                                                  <  5:364867/149‑1 (MQ=255)
                                                                   ctgctgctgCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATgg                                                                                                                                                <  2:246285/85‑1 (MQ=255)
                                                                   ctgctgctgCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATgg                                                                                                                                                >  1:246285/1‑85 (MQ=255)
                                                                           gcACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCtgctgc                                                                        >  5:90426/1‑149 (MQ=255)
                                                                            cacaCGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCg                                                                                      >  6:70406/1‑134 (MQ=255)
                                                                            cacaCGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCg                                                                                      <  5:70406/134‑1 (MQ=255)
                                                                                     gAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCg                                                              >  3:255204/1‑149 (MQ=255)
                                                                                             tGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTg                                                      >  1:180527/1‑149 (MQ=255)
                                                                                               aGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGTCTCGCTGCTGCGCGACACCCGTCCGGGTGGc                                                    <  7:1508/149‑1 (MQ=255)
                                                                                                   gcagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTg                                                                                                                                 <  8:196440/68‑1 (MQ=255)
                                                                                                   gcagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTg                                                                                                                                 >  7:196440/1‑68 (MQ=255)
                                                                                                    cagcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGGACGGGTGTCGcgca                                               <  3:53713/149‑1 (MQ=255)
                                                                                                                  gCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGAc                                        <  6:257434/142‑1 (MQ=255)
                                                                                                                  gCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGAc                                        >  5:257434/1‑142 (MQ=255)
                                                                                                                  gCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTcc                                    <  1:86999/146‑1 (MQ=255)
                                                                                                                  gCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTcc                                    >  2:86999/1‑146 (MQ=255)
                                                                                                                  gCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGc                                  >  6:265566/1‑148 (MQ=255)
                                                                                                                    ccaccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAAc                               >  2:300612/1‑149 (MQ=255)
                                                                                                                     caccaATTTCCTCAGGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACt                              <  4:114772/149‑1 (MQ=255)
                                                                                                                     caccaATTTCCTCAAGGCTCAGCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCACCt                              >  3:348475/1‑149 (MQ=255)
                                                                                                                     caccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACt                              >  7:63368/1‑149 (MQ=255)
                                                                                                                     caccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACt                              >  6:70185/1‑149 (MQ=255)
                                                                                                                     caccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACt                              >  3:233625/1‑149 (MQ=255)
                                                                                                                       ccaATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTc                            >  4:284942/1‑149 (MQ=255)
                                                                                                                               cTCAAGGCTCACCTTGAGTAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGc                    <  2:344296/149‑1 (MQ=255)
                                                                                                                               cTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACa                       <  8:309985/146‑1 (MQ=255)
                                                                                                                               cTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACa                       >  7:309985/1‑146 (MQ=255)
                                                                                                                               cTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGc                    <  2:180527/149‑1 (MQ=255)
                                                                                                                                   cggCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCACGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTaa                <  4:255204/148‑1 (MQ=255)
                                                                                                                                    ggCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAg               <  6:90426/149‑1 (MQ=255)
                                                                                                                                         aCCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGa                                         <  6:56951/118‑1 (MQ=255)
                                                                                                                                         aCCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGa                                         >  5:56951/1‑118 (MQ=255)
                                                                                                                                              gAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAgg      <  5:70185/148‑1 (MQ=255)
                                                                                                                                              gAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAg       <  3:284942/147‑1 (MQ=255)
                                                                                                                                                 cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCttt  >  3:137023/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACGCAACCTGTGGTGGTTCGAAGTGCCGGTGGCACTGCTGGGCAAGGGCCAGGCCGACTGGGTCAACCTGCTGCTGCACACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTT  >  NC_002947/5182612‑5182905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑