Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,201,830 C→T 8.9% A46V (GCC→GTC)  PP_4583 → putative Peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,201,8300CT 8.9% 125.3 / 2.4 45A46V (GCC→GTC) PP_4583putative Peptidase
Reads supporting (aligned to +/- strand):  ref base C (24/17);  new base T (2/2);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

GAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCCGCGTCAC  >  NC_002947/5201684‑5201969
                                                                                                                                                  |                                                                                                                                           
gagaCCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGcc                                                                                                                                            >  4:277479/1‑148 (MQ=255)
gagaCCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGcc                                                                                                                                            <  3:277479/148‑1 (MQ=255)
      ttCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCa                                                                                                                                       <  5:74891/147‑1 (MQ=255)
      ttCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCa                                                                                                                                       >  6:74891/1‑147 (MQ=255)
                  caaGCCCCAACCCCCGATTGTCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGt                                                                                                                          >  2:79032/1‑148 (MQ=255)
                  caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCag                                                                                                                                  >  4:54355/1‑139 (MQ=255)
                  caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCag                                                                                                                                  <  3:54355/140‑2 (MQ=255)
                  caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTg                                                                                                                         >  3:174587/1‑149 (MQ=255)
                     gCCCCAACCCCCGATTGTCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgc                                                                                                                      <  1:79032/149‑1 (MQ=255)
                       cccAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgcgc                                                                                                                    >  5:60538/1‑149 (MQ=255)
                        caaaCCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgcgc                                                                                                                    <  5:311932/146‑1 (MQ=255)
                         cAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCt                                                                                                                  >  1:79172/1‑149 (MQ=255)
                                     gCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAAt                                                                                                      >  6:359962/1‑149 (MQ=255)
                                               aCCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCgaa                                                                                                           <  3:53739/134‑1 (MQ=255)
                                               aCCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCgaa                                                                                                           >  4:53739/1‑134 (MQ=255)
                                                      cACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCagg                                                                                                                                 <  3:307212/105‑3 (MQ=255)
                                                      cACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCagg                                                                                                                                 >  4:307212/1‑103 (MQ=255)
                                                         cGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGgaga                                                                                  >  4:74296/1‑149 (MQ=255)
                                                                   gCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAGGAAATCAAGGGCCGCATCCTGGAGACCGACCTGt                                                                         >  8:167646/1‑148 (MQ=255)
                                                                       ggCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTg                                                                    >  2:201510/1‑149 (MQ=255)
                                                                           gCAACAGCGCGACACCCCCGAGGTACTCGGTTACCTGCAAGCTGAAAACGCCTACCAGTAAGACTGCCTGGCCTTCCAGGCACCGTTGCTTGAGCGCCTGTTCGAAGAAATCa                                                                                                    <  3:53709/113‑1 (MQ=255)
                                                                               cAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAg                                                                                                  >  7:61146/1‑111 (MQ=255)
                                                                               cAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAg                                                                                                  <  8:61146/111‑1 (MQ=255)
                                                                                 gcgcGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCt                                                          >  2:357674/1‑149 (MQ=255)
                                                                                  cgcgACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTg                                                         >  7:110814/1‑149 (MQ=255)
                                                                                    cgACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGACACCGACCTGTCATTGCCGGCGCCCTggg                                                       <  1:201510/149‑1 (MQ=255)
                                                                                        acCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGccc                                                   <  8:110814/149‑1 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCAc                                                                                                                                  >  4:119154/1‑68 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCAc                                                                                                                                  <  3:119154/68‑1 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                  >  8:297001/1‑148 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                  >  7:204469/1‑148 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                  >  6:316552/1‑148 (MQ=255)
                                                                                          ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                  >  4:10986/1‑148 (MQ=255)
                                                                                           cccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTa                                                >  6:151594/1‑149 (MQ=255)
                                                                                           cccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTa                                                <  1:357674/149‑1 (MQ=255)
                                                                                                    cTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtact                                        <  8:204469/148‑1 (MQ=255)
                                                                                                       gCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTacac                                    >  7:120812/1‑149 (MQ=255)
                                                                                                       gCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTaca                                     <  2:79172/148‑1 (MQ=255)
                                                                                                             cTGCAAGCTGAAATCGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTac                              <  7:297001/149‑1 (MQ=255)
                                                                                                              tGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtac                                         <  8:12131/137‑1 (MQ=255)
                                                                                                              tGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtac                                         >  7:12131/1‑137 (MQ=255)
                                                                                                                cAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTacc                             <  6:60538/147‑1 (MQ=255)
                                                                                                                                aCCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCATGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCGCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAatat           >  2:192198/1‑149 (MQ=255)
                                                                                                                                    ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGcc                                                            >  7:235686/1‑96 (MQ=255)
                                                                                                                                    ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGcc                                                            <  8:235686/96‑1 (MQ=255)
                                                                                                                                    ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCcgc       <  4:174587/149‑1 (MQ=255)
                                                                                                                                          ctgcctgGCCGACCAGGCCCCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACATGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGCCTATCCGCGTccc  >  7:88901/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCCGCGTCAC  >  NC_002947/5201684‑5201969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑