Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,201,833 A→G 8.9% D47G (GAC→GGC)  PP_4583 → putative Peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,201,8330AG 8.9% 116.6 / 3.3 46D47G (GAC→GGC) PP_4583putative Peptidase
Reads supporting (aligned to +/- strand):  ref base A (24/17);  new base G (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCCGCGTCACTACCGCTGCCC  >  NC_002947/5201690‑5201980
                                                                                                                                               |                                                                                                                                                   
ttCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCa                                                                                                                                                  <  5:74891/147‑1 (MQ=255)
ttCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCa                                                                                                                                                  >  6:74891/1‑147 (MQ=255)
            caaGCCCCAACCCCCGATTGTCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGt                                                                                                                                     >  2:79032/1‑148 (MQ=255)
            caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCag                                                                                                                                             >  4:54355/1‑139 (MQ=255)
            caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCag                                                                                                                                             <  3:54355/140‑2 (MQ=255)
            caaGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTg                                                                                                                                    >  3:174587/1‑149 (MQ=255)
               gCCCCAACCCCCGATTGTCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgc                                                                                                                                 <  1:79032/149‑1 (MQ=255)
                 cccAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgcgc                                                                                                                               >  5:60538/1‑149 (MQ=255)
                  caaaCCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAgcgc                                                                                                                               <  5:311932/146‑1 (MQ=255)
                   cAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCt                                                                                                                             >  1:79172/1‑149 (MQ=255)
                               gCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAAt                                                                                                                 >  6:359962/1‑149 (MQ=255)
                                         aCCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCgaa                                                                                                                      <  3:53739/134‑1 (MQ=255)
                                         aCCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCgaa                                                                                                                      >  4:53739/1‑134 (MQ=255)
                                                cACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCagg                                                                                                                                            <  3:307212/105‑3 (MQ=255)
                                                cACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGTCGGCCAGGCagg                                                                                                                                            >  4:307212/1‑103 (MQ=255)
                                                   cGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGgaga                                                                                             >  4:74296/1‑149 (MQ=255)
                                                             gCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAGGAAATCAAGGGCCGCATCCTGGAGACCGACCTGt                                                                                    >  8:167646/1‑148 (MQ=255)
                                                                 ggCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTg                                                                               >  2:201510/1‑149 (MQ=255)
                                                                     gCAACAGCGCGACACCCCCGAGGTACTCGGTTACCTGCAAGCTGAAAACGCCTACCAGTAAGACTGCCTGGCCTTCCAGGCACCGTTGCTTGAGCGCCTGTTCGAAGAAATCa                                                                                                               <  3:53709/113‑1 (MQ=255)
                                                                         cAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAg                                                                                                             >  7:61146/1‑111 (MQ=255)
                                                                         cAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAg                                                                                                             <  8:61146/111‑1 (MQ=255)
                                                                           gcgcGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCt                                                                     >  2:357674/1‑149 (MQ=255)
                                                                            cgcgACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTg                                                                    >  7:110814/1‑149 (MQ=255)
                                                                              cgACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGACACCGACCTGTCATTGCCGGCGCCCTggg                                                                  <  1:201510/149‑1 (MQ=255)
                                                                                  acCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGccc                                                              <  8:110814/149‑1 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCAc                                                                                                                                             >  4:119154/1‑68 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCAc                                                                                                                                             <  3:119154/68‑1 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                             >  8:297001/1‑148 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                             >  7:204469/1‑148 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                             >  4:10986/1‑148 (MQ=255)
                                                                                    ccccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGcccc                                                             >  6:316552/1‑148 (MQ=255)
                                                                                     cccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTa                                                           >  6:151594/1‑149 (MQ=255)
                                                                                     cccGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTa                                                           <  1:357674/149‑1 (MQ=255)
                                                                                              cTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtact                                                   <  8:204469/148‑1 (MQ=255)
                                                                                                 gCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTacac                                               >  7:120812/1‑149 (MQ=255)
                                                                                                 gCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTaca                                                <  2:79172/148‑1 (MQ=255)
                                                                                                       cTGCAAGCTGAAATCGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTac                                         <  7:297001/149‑1 (MQ=255)
                                                                                                        tGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtac                                                    <  8:12131/137‑1 (MQ=255)
                                                                                                        tGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGtac                                                    >  7:12131/1‑137 (MQ=255)
                                                                                                          cAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTacc                                        <  6:60538/147‑1 (MQ=255)
                                                                                                                          aCCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCATGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCGCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAatat                      >  2:192198/1‑149 (MQ=255)
                                                                                                                              ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGcc                                                                       >  7:235686/1‑96 (MQ=255)
                                                                                                                              ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGcc                                                                       <  8:235686/96‑1 (MQ=255)
                                                                                                                              ggAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCcgc                  <  4:174587/149‑1 (MQ=255)
                                                                                                                                    ctgcctgGCCGACCAGGCCCCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACATGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGCCTATCCGCGTccc             >  7:88901/1‑146 (MQ=255)
                                                                                                                                              gACCATGCACCGTTGCGTGAGCGCCTGTTCGATGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCCGCGTCACTACCGCTGccc  <  7:167646/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCGAAGAAATCAAGGGCCGCATCCTGGAGACCGACCTGTCATTGCCGGCGCCCTGGGGCCCCTACCTGTACTACACCCGTACCACCGCCGGCGACGAATATCCGCGTCACTACCGCTGCCC  >  NC_002947/5201690‑5201980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑