Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,232,057 C→G 10.9% P213P (CCG→CCC)  PP_4610 ← PepSY‑associated TM helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,232,0570CG10.9% 131.8 / 7.1 46P213P (CCG→CCC) PP_4610PepSY‑associated TM helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base G (2/3);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGATC  >  NC_002947/5231913‑5232204
                                                                                                                                                |                                                                                                                                                   
cTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCa                                                                                                                                                 <  3:297491/149‑1 (MQ=255)
         cTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCat                                                                                                                                             >  6:153711/1‑143 (MQ=255)
         cTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCat                                                                                                                                             <  5:153711/144‑2 (MQ=255)
                  ggCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAAc                                                                                                                                <  2:171578/148‑1 (MQ=255)
                      tACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatg                                                                                                                                            <  4:177688/132‑3 (MQ=255)
                      tACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatg                                                                                                                                            >  3:177688/1‑130 (MQ=255)
                        cAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTTGTCCGCCTTGGGCGCATCGTTGCTGCCAAACAGGTCGTTGATGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCTGCATCACCATGTACATGAACAGCAcc                                                                                                                          <  6:354494/148‑1 (MQ=255)
                        cAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCa                                                                                                                         <  8:65530/149‑1 (MQ=255)
                             tCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGgctg                                                                                                                    >  5:21859/1‑149 (MQ=255)
                               ggCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGgctg                                                                                                                    <  5:15047/147‑1 (MQ=255)
                                    cgcTACCTGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGc                                                                                                             <  5:122970/149‑1 (MQ=255)
                                     gcTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCt                                                                                                            >  6:163617/1‑149 (MQ=255)
                                      cTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTg                                                                                                           <  3:84954/149‑1 (MQ=255)
                                          cAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATc                                                                                                                                               <  4:234628/109‑1 (MQ=255)
                                          cAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATc                                                                                                                                               >  3:234628/1‑109 (MQ=255)
                                          cAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatca                                                                                                       >  1:15627/1‑149 (MQ=255)
                                             cggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatcat                                                                                                      >  1:297204/1‑147 (MQ=255)
                                             cggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                    <  6:67425/149‑1 (MQ=255)
                                                               cgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCAGCCTGGTGCTGttc                                                                                                              >  4:313289/1‑119 (MQ=255)
                                                                   ttGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcaccagcac                                                                              >  5:321823/1‑149 (MQ=255)
                                                                     gggCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGc                                                                            >  4:30305/1‑149 (MQ=255)
                                                                      ggCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGc                                                                            <  2:297204/148‑1 (MQ=255)
                                                                       gcgcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCg                                                                          <  2:15627/149‑1 (MQ=255)
                                                                          cATCGTTGCTGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATTAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATg                                                                       <  7:172962/149‑1 (MQ=255)
                                                                          cATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATg                                                                       <  3:30305/149‑1 (MQ=255)
                                                                                  cGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACgg                                                                                          <  1:211163/122‑1 (MQ=255)
                                                                                  cGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACgg                                                                                          >  2:211163/1‑122 (MQ=255)
                                                                                   ggCCAAACAGGTCGTTGAAGTACTCGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGc                                                              <  2:158154/149‑1 (MQ=255)
                                                                                    gCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGcc                                                             <  3:40488/149‑1 (MQ=255)
                                                                                     ccAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGccc                                                            >  5:15600/1‑149 (MQ=255)
                                                                                     ccAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGc                                                              <  6:21859/147‑1 (MQ=255)
                                                                                      cAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCg                                                           >  6:345384/1‑149 (MQ=255)
                                                                                              tCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGt                                                                                                 >  2:168532/1‑103 (MQ=255)
                                                                                              tCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGt                                                                                                 <  1:168532/103‑1 (MQ=255)
                                                                                                   gAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAAc                                              >  5:155194/1‑149 (MQ=255)
                                                                                                       tACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTg                                          >  5:230553/1‑149 (MQ=255)
                                                                                                                 tgtCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGc                                  >  3:310142/1‑147 (MQ=255)
                                                                                                                   tCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAgg                              <  6:230553/149‑1 (MQ=255)
                                                                                                                     aTTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGc                            <  6:321823/149‑1 (MQ=255)
                                                                                                                         ccGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCgg                          >  7:155938/1‑147 (MQ=255)
                                                                                                                          cGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAg                       <  5:163617/149‑1 (MQ=255)
                                                                                                                               tacatggtgatgCCGGCGGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaa                  <  1:133819/142‑1 (MQ=255)
                                                                                                                                 ggCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaag                 >  4:40842/1‑148 (MQ=255)
                                                                                                                                     atgatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGc                                  <  6:2368/127‑1 (MQ=255)
                                                                                                                                     atgatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGc                                  >  5:2368/1‑127 (MQ=255)
                                                                                                                                           cccGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGa       <  1:244389/148‑1 (MQ=255)
                                                                                                                                             cgccgGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTg        >  2:167645/1‑145 (MQ=255)
                                                                                                                                               ccgGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGAtc  <  5:345384/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGATC  >  NC_002947/5231913‑5232204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑