Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,262,734 G→A 7.8% A303T (GCC→ACC)  PP_4638 → methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,262,7340GA 7.8% 143.6 / 2.3 51A303T (GCC→ACC) PP_4638methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base A (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCACTGCTGTTC  >  NC_002947/5262586‑5262877
                                                                                                                                                     |                                                                                                                                               
gCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACg                                                                                                                                                 >  2:155518/1‑149 (MQ=255)
    aTTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACTCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAg                                                                                                                                             >  3:284704/1‑149 (MQ=255)
      tATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGc                                                                                                                                            <  1:155518/148‑1 (MQ=255)
          ggTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTgg                                                                                                                                         >  7:73451/1‑147 (MQ=255)
            tACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑CGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCata                                                                                                                                     >  8:137591/1‑147 (MQ=255)
               aaaGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATgc                                                                                                                                  >  6:106101/1‑149 (MQ=255)
                  gCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACTCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATgcctg                                                                                                                               <  4:284704/149‑1 (MQ=255)
                     gATGGTATACGACTGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGATGGCACTTGAGACCCGTATCGAGTACTGGCAAACACA‑GGTTCAGTCACTGGAGCAATGCGCACCGGACTGGCACGCCAGCTTGCAGATgcctgca                                                                                                                             >  2:269809/1‑146 (MQ=255)
                           cTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCcgg                                                                                                                      >  7:25924/1‑149 (MQ=255)
                               gCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGt                                                                                                                  <  3:93703/149‑1 (MQ=255)
                               gCCGGGGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCTGt                                                                                                                  <  4:203279/149‑1 (MQ=255)
                                      tgCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCtt                                                                                                            <  7:47650/148‑1 (MQ=255)
                                           cTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATcc                                                                                                      <  7:137591/149‑1 (MQ=255)
                                                aGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCca                                                                                                  >  5:303667/1‑148 (MQ=255)
                                                         cacGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAggctggct                                                                                        >  2:207163/1‑149 (MQ=255)
                                                             cTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGgcg                                                                                    <  8:25924/149‑1 (MQ=255)
                                                              tCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGgcg                                                                                    >  3:48991/1‑148 (MQ=255)
                                                                gAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCAGGGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGcc                                                                                  >  1:80054/1‑149 (MQ=255)
                                                                gAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGgcg                                                                                    <  4:48991/146‑1 (MQ=255)
                                                                gAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCt                                                                                 <  1:207163/149‑1 (MQ=255)
                                                                  aCAGCTGCTAGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTgg                                                                               <  7:77706/149‑1 (MQ=255)
                                                                    aGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGAc                                                                             >  3:309637/1‑149 (MQ=255)
                                                                       tgctgGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAg                                                                          >  7:212642/1‑149 (MQ=255)
                                                                             cccTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACgg                                                                    >  5:309986/1‑149 (MQ=255)
                                                                              ccTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACggg                                                                   >  8:297402/1‑149 (MQ=255)
                                                                                  gagaCCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGa                                                               <  2:201120/149‑1 (MQ=255)
                                                                                    gaCCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATgcctggcct                                                                                                                           <  6:120739/87‑1 (MQ=255)
                                                                                    gaCCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATgcctggcct                                                                                                                           >  5:120739/1‑87 (MQ=255)
                                                                                       ccGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACACCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCt                                                          >  8:44695/1‑149 (MQ=255)
                                                                                             cGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACgg                                                                    <  1:99876/133‑1 (MQ=255)
                                                                                             cGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACgg                                                                    >  2:99876/1‑133 (MQ=255)
                                                                                                 tACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAgg                                                 >  2:293006/1‑148 (MQ=255)
                                                                                                      gCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACACCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCAc                                                                      >  8:179163/1‑122 (MQ=255)
                                                                                                      gCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACACCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCAc                                                                      <  7:179163/122‑1 (MQ=255)
                                                                                                                  ttCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGTCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCAc                                <  5:288760/149‑1 (MQ=255)
                                                                                                                      gTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGa                            >  6:101333/1‑149 (MQ=255)
                                                                                                                       tCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGAt                           <  5:106101/149‑1 (MQ=255)
                                                                                                                             gAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATgcatg                      <  5:327663/148‑1 (MQ=255)
                                                                                                                             gAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATgcat                       <  5:101333/147‑1 (MQ=255)
                                                                                                                                cAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATgcatggcat                  <  4:309637/149‑1 (MQ=255)
                                                                                                                                 aaTGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATgcatggcatg                 >  3:101053/1‑149 (MQ=255)
                                                                                                                                   tGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATgca               <  6:303667/149‑1 (MQ=255)
                                                                                                                                    gggCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATgcac              >  7:263301/1‑149 (MQ=255)
                                                                                                                                     ggCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATgcac              <  8:73451/148‑1 (MQ=255)
                                                                                                                                        aCCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATgcacgcac          >  7:162216/1‑149 (MQ=255)
                                                                                                                                        aCCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATgcacgcac          >  3:308940/1‑149 (MQ=255)
                                                                                                                                            gCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCActgct      <  7:297402/149‑1 (MQ=255)
                                                                                                                                            gCCTGGCACACCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCActgct      <  7:44695/149‑1 (MQ=255)
                                                                                                                                              cTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCACTGCTGt    >  6:70337/1‑149 (MQ=255)
                                                                                                                                                 gCACGCCAGCTTGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCACTGCTGTTc  <  8:263301/148‑1 (MQ=255)
                                                                                                                                                 gCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGcc                                                                                  >  2:158688/1‑68 (MQ=255)
                                                                                                                                                 gCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGcc                                                                                  <  1:158688/68‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAACCCA‑GGTTCAGTCCCTGGAGCAATGGGCACCGGCCTGGCACGCCAGCTGGCAGATGCCTGGCCTGCCGGCGGTGACCTTCCATCCGCCACAGGCTGGCTGGCGCCTGGACGAGTCACGGGTCGATGCCTCATTCAAGGAAAAAGCCCGCTATCACCTGATGCATGGCATGCACGCACTGCTGTTC  >  NC_002947/5262586‑5262877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑