Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,283,026	T→G	6.5%	F185V (TTC→GTC)	ypfJ →	zinc metalloprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,283,026	0	T	G	6.5%	191.7 / 4.4	62	F185V (TTC→GTC)	ypfJ	zinc metalloprotease
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base G (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

TGTTCAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAGGAGTTGCAGGCTGA  >  NC_002947/5282886‑5283174                                                                                                                                              |                                                                                                                                                      tGTTCAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTGACCCGGCGGACAAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGcc                                                                                                                                                  >  1:286299/1‑147 (MQ=255)  gTTCGGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAgg                                                                                                                                              >  2:202467/1‑149 (MQ=255)  gTTCAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAgg                                                                                                                                              >  5:180373/1‑149 (MQ=255)   ttCAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAgg                                                                                                                                              <  2:242665/148‑1 (MQ=255)   cgcAGCGGCCAGGGCAATTCCGCCTGCGGTTTTGCCTCGCCAGCGGTCGGGCCGTTCTATTT‑CCCGGCGGACCAGCGGGTGTACCTGGACAAGTCGTTCTTCCGCGAAATGGAAACCCGCCTCGCTGCAGCGGGCGACTTCGCCCAGGc                                                                                                                                             <  4:212896/147‑1 (MQ=255)     cAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGcc                                                                                                                                            >  8:303113/1‑148 (MQ=255)       gCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTAc                                                                                                                                         <  4:4977/149‑1 (MQ=255)           ccAGGTCAATTCGGCCTGTGGTTTTGCCGCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAACCCCCCTTCGCTGCTGCGGGCGACTTCGCCCAGGCCTACGTGa                                                                                                                                     <  4:36851/149‑1 (MQ=255)             aGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATc                                                                                                                                   >  8:140855/1‑149 (MQ=255)               gTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCGGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGc                                                                                                                                 >  1:168003/1‑149 (MQ=255)               gTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATc                                                                                                                                   >  4:48839/1‑147 (MQ=255)               gTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATc                                                                                                                                   <  3:48839/147‑1 (MQ=255)               gTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGc                                                                                                                                 <  7:303113/149‑1 (MQ=255)                tCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCg                                                                                                                                                    >  7:283921/1‑129 (MQ=255)                tCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCg                                                                                                                                                    <  8:283921/129‑1 (MQ=255)                           tgtgGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTg                                                                                                                                      <  2:274551/132‑1 (MQ=255)                           tgtgGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTg                                                                                                                                      >  1:274551/1‑132 (MQ=255)                             tgGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCTGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTc                                                                                                                   <  2:168003/149‑1 (MQ=255)                                      cTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCAtgtg                                                                                                           >  6:162978/1‑148 (MQ=255)                                      cTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCAtgtg                                                                                                           >  3:330486/1‑148 (MQ=255)                                           cAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGaca                                                                                                     <  5:162978/149‑1 (MQ=255)                                               ggTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACActgc                                                                                                 >  8:125075/1‑149 (MQ=255)                                                  cGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTgg                                                                                              <  7:140855/149‑1 (MQ=255)                                                   gggCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTggg                                                                                             <  3:255828/149‑1 (MQ=255)                                                   gggCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTggg                                                                                             <  2:358031/149‑1 (MQ=255)                                                    ggCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGc                                                                                            >  4:301825/1‑149 (MQ=255)                                                                  cccGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAg                                                                                <  4:82247/148‑1 (MQ=255)                                                                        ggACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑g                                                                        <  3:301825/149‑1 (MQ=255)                                                                        ggACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑g                                                                        <  3:27992/149‑1 (MQ=255)                                                                              gCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGcc                                                                                                                                                  >  7:279271/1‑70 (MQ=255)                                                                              gCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGcc                                                                                                                                                  <  8:279271/70‑1 (MQ=255)                                                                                        cTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCg                                                                                                                      <  6:348951/88‑1 (MQ=255)                                                                                        cTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCg                                                                                                                      >  5:348951/1‑88 (MQ=255)                                                                                        cTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCgg                                                        >  2:281536/1‑149 (MQ=255)                                                                                           gACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGc                                                       >  5:68902/1‑147 (MQ=255)                                                                                                gTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACActg                                                                                                  <  4:177380/100‑1 (MQ=255)                                                                                                gTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACActg                                                                                                  >  3:177380/1‑100 (MQ=255)                                                                                                     tcttcCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAg                                           >  4:20267/1‑149 (MQ=255)                                                                                                      cttcCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCAaggagg                                          >  2:102463/1‑149 (MQ=255)                                                                                                       ttcCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACGCTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAggg                                         >  6:210622/1‑149 (MQ=255)                                                                                                        tcCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAgg                                          <  1:35608/147‑1 (MQ=255)                                                                                                        tcCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAgg                                          >  2:35608/1‑147 (MQ=255)                                                                                                         ccGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGc                                        <  6:180373/148‑1 (MQ=255)                                                                                                           gcgAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACCCTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGAc                                     <  5:210622/149‑1 (MQ=255)                                                                                                                  ggAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGcc                              >  7:45397/1‑149 (MQ=255)                                                                                                                   gAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCt                             <  3:20267/149‑1 (MQ=255)                                                                                                                    aaaCCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGTCCTg                            <  7:125075/149‑1 (MQ=255)                                                                                                                      aCCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACgcgc                                                           >  6:16441/1‑116 (MQ=255)                                                                                                                      aCCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACgcgc                                                           <  5:16441/116‑1 (MQ=255)                                                                                                                       cccGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTg                         <  6:68902/149‑1 (MQ=255)                                                                                                                               gcagCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACCGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGACTGTTGGTGCGCCa                 >  8:297893/4‑149 (MQ=255)                                                                                                                                cTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAg                >  2:115278/1‑149 (MQ=255)                                                                                                                                 tGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAg                >  5:345063/1‑148 (MQ=255)                                                                                                                                 aggcctgggCGAAGTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTgg                        <  6:207229/134‑1 (MQ=255)                                                                                                                                 aggcctgggCGAAGTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTgg                        >  5:207229/7‑140 (MQ=255)                                                                                                                                   cAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGACGACAACGGCCTGTTGGTGCGCCAGGAg             <  1:202467/149‑1 (MQ=255)                                                                                                                                    aGCGGGCGACTTCGCCCTGGCCTACGTGATCGCCCACGAAATCGGGCACCATGTGCAGACACTGCTGGGCGTTTCGGCAAAGGTGTATCACCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAGGAg             <  1:102463/149‑1 (MQ=255)                                                                                                                                     ctgggCGAAGTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCgg                                                                                     >  7:102255/3‑76 (MQ=255)                                                                                                                                     ctgggCGAAGTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCgg                                                                                     <  8:102255/74‑1 (MQ=255)                                                                                                                                           gACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAGGAGTTGCAGGc     >  3:226365/1‑149 (MQ=255)                                                                                                                                             cTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGc                                        <  6:145258/112‑1 (MQ=255)                                                                                                                                             cTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGc                                        >  5:145258/1‑112 (MQ=255)                                                                                                                                              ttCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCg                                       >  6:231240/1‑112 (MQ=255)                                                                                                                                              ttCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCg                                       <  5:231240/112‑1 (MQ=255)                                                                                                                                              ttCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAGGAGTTGCAGGCTGa  >  1:243483/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                      TGTTCAGCGGCCAGGTCAATTCGGCCTGTGGTTTTGCCTCGGCAGCGGTCGGGCCGTTCTATTG‑CCCGGCGGACCAGCGGGTGTACCTGGACATGTCGTTCTTCCGCGAAATGGAAACCCGCTTCGCTGCAGCGGGCGACTTCGCCCAGGCCTACGTGATCGCCCACGAAATCGGTCACCATGTGCAGACACTGCTGGGCGTTTCGGCCAAGGTGGAT‑GCCGCACGACGCGCCGGCCAGCGCATGGAGGGCGACAACGGCCTGTTGGTGCGCCAGGAGTTGCAGGCTGA  >  NC_002947/5282886‑5283174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑