Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,305,668 C→T 6.1% A528A (GCG→GCA)  recC ← multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,305,6680CT 6.1% 244.2 / 3.7 81A528A (GCG→GCA) recCmultifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit
Reads supporting (aligned to +/- strand):  ref base C (41/35);  new base T (3/2);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

GATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACCGCGAAGCGG  >  NC_002947/5305521‑5305815
                                                                                                                                                    |                                                                                                                                                     
gATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCg                                                                                                                                                      >  6:52125/1‑149 (MQ=255)
        ccGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCGAGCCTGGGCTTGCCCGccgg                                                                                                                                              <  8:35034/149‑4 (MQ=255)
         cgcgTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTgc‑g                                                                                                                                            >  1:270176/1‑149 (MQ=255)
           cgTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGc‑gcg                                                                                                                                          >  5:283289/1‑149 (MQ=255)
             tCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑gcggc                                                                                                                                        <  7:80847/149‑1 (MQ=255)
                aGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTc                                                                                                                                     <  3:205977/149‑1 (MQ=255)
                        gATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGcccc                                                                                                                             <  6:283289/149‑1 (MQ=255)
                            tCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACACCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACg                                                                                                                         >  5:227908/1‑149 (MQ=255)
                              aTCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAACCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGCCACGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACg                                                                                                                         >  1:98444/1‑148 (MQ=255)
                               tCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGAt                                                                                                                      >  5:195906/1‑149 (MQ=255)
                               tCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACACCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGAt                                                                                                                      <  6:227908/149‑1 (MQ=255)
                                   aGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGc                                                                                                                    >  6:185537/1‑147 (MQ=255)
                                       ttCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAgcgc                                                                                                              >  1:218274/1‑149 (MQ=255)
                                       ttCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAgcgc                                                                                                              >  1:213001/1‑149 (MQ=255)
                                          aaTGCGATGACAGGCCTCACCCACCCCCACGTCATAACCCATC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCTATCCCGGCTTGCAAGCCCAGTCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCt                                                                                                           <  7:260938/149‑1 (MQ=255)
                                          aaTGCCATCACAGGCCTCACCCATCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCt                                                                                                           >  5:35080/1‑149 (MQ=255)
                                             gCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGa                                                                                                       >  7:169966/1‑149 (MQ=255)
                                             gCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCCCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGa                                                                                                       >  1:309878/1‑149 (MQ=255)
                                               cATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATc                                                                                                     >  8:52099/1‑149 (MQ=255)
                                                aTCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑Cg                                                                                                        >  5:205364/1‑145 (MQ=255)
                                                aTCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑Cg                                                                                                        <  6:205364/145‑1 (MQ=255)
                                                    caGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCg                                                                                                <  5:185537/149‑1 (MQ=255)
                                                            acccacccCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCACCg                                                                                        >  7:116513/1‑149 (MQ=255)
                                                             cccacccCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGt                                                                                       >  8:29896/1‑149 (MQ=255)
                                                             cccacccCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGt                                                                                       <  8:136509/149‑1 (MQ=255)
                                                                acccCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCGCT‑CGATCCAGCGGTGCAGCGTggg                                                                                    <  7:52099/149‑1 (MQ=255)
                                                                  ccccACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCACGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCCGCGCCCT‑CGATCCAGCGGTCCAGCGgggg                                                                                    >  8:264068/1‑147 (MQ=255)
                                                                    ccACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAgg                                                                                >  2:352745/1‑149 (MQ=255)
                                                                     cACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGt                                                                               <  6:195906/149‑1 (MQ=255)
                                                                     cACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGt                                                                               >  6:255656/1‑149 (MQ=255)
                                                                              aCCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGCTCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgc                                                                      >  2:230686/1‑149 (MQ=255)
                                                                              aCCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGt                                                                                       <  3:61253/132‑1 (MQ=255)
                                                                              aCCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGt                                                                                       >  4:61253/1‑132 (MQ=255)
                                                                               cccAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAg                                                                                 <  4:295792/137‑1 (MQ=255)
                                                                               cccAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAg                                                                                 >  3:295792/1‑137 (MQ=255)
                                                                               cccAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgc                                                                      >  5:275792/1‑148 (MQ=255)
                                                                               cccAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgc                                                                      <  6:275792/148‑1 (MQ=255)
                                                                                ccAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg                                                                     >  1:177530/1‑148 (MQ=255)
                                                                                          tGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAg                                                           <  1:352745/149‑1 (MQ=255)
                                                                                           gcggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGa                                                                                                       <  4:96981/104‑1 (MQ=255)
                                                                                           gcggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGa                                                                                                       >  3:96981/1‑104 (MQ=255)
                                                                                            cggcgCAGGCCGAAACGCCAACTGGTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGc                                                          <  8:67098/148‑1 (MQ=255)
                                                                                             ggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg                                                        <  5:52125/149‑1 (MQ=255)
                                                                                                    gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGa                                                 <  2:218274/149‑1 (MQ=255)
                                                                                                    gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGa                                                 >  4:56423/1‑149 (MQ=255)
                                                                                                    gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGa                                                 <  2:213001/149‑1 (MQ=255)
                                                                                                     ccGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg                                                        <  6:345527/141‑1 (MQ=255)
                                                                                                     ccGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg                                                        >  5:345527/1‑141 (MQ=255)
                                                                                                      cGAAACGCGAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTCCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACt                                               >  6:228292/1‑149 (MQ=255)
                                                                                                      cGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACt                                               >  4:273759/1‑149 (MQ=255)
                                                                                                           cGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgt                                          <  2:177530/149‑2 (MQ=255)
                                                                                                           cGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                          <  3:56423/149‑1 (MQ=255)
                                                                                                           cGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                          <  6:35080/149‑1 (MQ=255)
                                                                                                           cGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                          <  1:230686/149‑1 (MQ=255)
                                                                                                            gCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                          >  1:269028/1‑149 (MQ=255)
                                                                                                             ccAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCa                                        >  4:164523/1‑149 (MQ=255)
                                                                                                               aaCTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACg                                      <  5:228292/149‑1 (MQ=255)
                                                                                                                 ctgttctgttCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTc                                    <  2:270176/149‑1 (MQ=255)
                                                                                                                    ttctgttcGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                                 >  1:266342/1‑149 (MQ=255)
                                                                                                                    ttctgttcGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                                 >  5:35001/1‑149 (MQ=255)
                                                                                                                      ctgttcGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                                 >  6:136330/1‑147 (MQ=255)
                                                                                                                           cGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTc                           <  8:169966/148‑1 (MQ=255)
                                                                                                                           cGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTc                           >  8:124496/1‑148 (MQ=255)
                                                                                                                            gAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTc                           >  3:250284/1‑147 (MQ=255)
                                                                                                                             aGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg                        <  6:35001/149‑1 (MQ=255)
                                                                                                                              gcgcagcggGCGAGCCTGGGCTTGCCCGCCGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg                        <  8:227737/143‑1 (MQ=255)
                                                                                                                              gcgcagcggGCGAGCCTGGGCTTGCCCGCCGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg                        >  7:227737/6‑148 (MQ=255)
                                                                                                                              gCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGc                       >  8:141150/1‑149 (MQ=255)
                                                                                                                               cccGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCa                      >  8:177424/1‑149 (MQ=255)
                                                                                                                                ccGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCAc                     <  7:124496/149‑1 (MQ=255)
                                                                                                                                   gcggGCGAGCCTGGGCTTGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTc                  >  4:84320/1‑149 (MQ=255)
                                                                                                                                    cggGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCg                 <  3:273759/149‑1 (MQ=255)
                                                                                                                                     gggCGAGCCTGGGCTTGCCCGCCGG‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGc                >  1:164330/1‑149 (MQ=255)
                                                                                                                                         aaGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTGCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa             >  1:277906/1‑149 (MQ=255)
                                                                                                                                         aaGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGAc            <  2:98444/149‑1 (MQ=255)
                                                                                                                                          aGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGAcc           <  5:136330/149‑1 (MQ=255)
                                                                                                                                           gCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCAGGCGCGGACt                                               <  7:184652/112‑1 (MQ=255)
                                                                                                                                            cccAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGcggtcagc         <  7:264068/149‑8 (MQ=255)
                                                                                                                                             ccAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACcgcg        <  5:255656/149‑1 (MQ=255)
                                                                                                                                                 gCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTAGCTGACCGCGAAGc    >  2:135956/1‑149 (MQ=255)
                                                                                                                                                   tCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACCGCGAAGCgg  >  8:94182/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAAC‑AGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGC‑GCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCT‑CGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACCGCGAAGCGG  >  NC_002947/5305521‑5305815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑