Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,313,279 C→G 11.4% intergenic (‑598/+116) PP_16SG ← / ← msrQ 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,313,2790CG11.4% 140.0 / 4.4 50intergenic (‑598/+116)PP_16SG/msrQ16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base G (2/4);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCC  >  NC_002947/5313138‑5313420
                                                                                                                                             |                                                                                                                                             
aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                        <  7:157871/149‑1 (MQ=255)
aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                        <  1:204944/149‑1 (MQ=255)
     gggCGCATTCTAAGGGGCTCCGAGAGGGCGTCAACCTGAAATTTCAAGACAGTGAAATATCAGGGGAAATCCCGTATCGGGGCTGCCACGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt                                                                                                                                   <  7:80335/149‑1 (MQ=14)
     gggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                                    >  8:8100/1‑148 (MQ=255)
     gggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                                    <  2:194419/148‑1 (MQ=255)
      ggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                  >  1:160889/1‑149 (MQ=255)
           aTTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCATGTAAGTGAAATATAAGGGGAAATACCGTATCGGGGGTGGCAAGTGTTCGCCACAACAGTTTCAGCGACTATGAGATCGAGCGCCGCCAGCGCGGCGCTCGATCTca                                                                                                                               <  6:157883/147‑1 (MQ=17)
           aTTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcat                                                                                                                              <  7:8100/148‑2 (MQ=255)
                  ggggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  4:80964/1‑147 (MQ=255)
                   gggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  6:332795/1‑146 (MQ=255)
                   gggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  8:150938/1‑146 (MQ=255)
                      aTTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCagag                                                                                                                   >  7:10078/1‑148 (MQ=255)
                               ggCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGc                                                                                                         <  6:152089/149‑1 (MQ=255)
                                     aaCCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGcc                                                                                                    <  7:283989/148‑1 (MQ=255)
                                           tAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCtctc                                                                                               <  1:45008/147‑1 (MQ=255)
                                                  aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAt                                                                                      >  5:165312/1‑149 (MQ=255)
                                                  aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAt                                                                                      >  3:78578/1‑149 (MQ=255)
                                                  aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTACCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAt                                                                                      >  1:136515/1‑149 (MQ=255)
                                                   aGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAtt                                                                                     >  1:16146/1‑149 (MQ=255)
                                                     aaaGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCCCAGCCGCCAGAGAAACAAAGGCGGGCCATCTCAACAGCCATTGc                                                                                   >  8:339980/1‑149 (MQ=255)
                                                         tGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtc                                                                               >  1:197765/1‑149 (MQ=255)
                                                          gAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCAAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtct                                                                              >  1:101091/1‑149 (MQ=255)
                                                           aaaTATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtct                                                                              <  2:197765/148‑1 (MQ=255)
                                                                   ggggAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAgg                                                                     <  2:160889/149‑1 (MQ=255)
                                                                        aaTACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                                <  6:165312/149‑1 (MQ=255)
                                                                        aaTACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                                <  4:78578/149‑1 (MQ=255)
                                                                         aTACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTg                                                               <  7:150938/149‑1 (MQ=255)
                                                                         aTACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCAAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTg                                                               <  2:101091/149‑1 (MQ=255)
                                                                                 tCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACGGGCCCTTGGCAGccc                                                        <  8:10078/148‑1 (MQ=255)
                                                                                    gggCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccacc                                                    <  5:332795/149‑1 (MQ=255)
                                                                                    gggCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccacc                                                    <  3:80964/149‑1 (MQ=255)
                                                                                     ggCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccaccc                                                   <  7:339980/149‑1 (MQ=255)
                                                                                     ggCTACCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccaccc                                                   <  2:136515/149‑1 (MQ=255)
                                                                                            aaGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccac                                                     >  4:188327/1‑140 (MQ=255)
                                                                                            aaGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGACAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccac                                                     <  3:188327/140‑1 (MQ=255)
                                                                                                     ccACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGcc                                    >  2:150459/1‑148 (MQ=255)
                                                                                                           caGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCt                             <  1:150459/149‑1 (MQ=255)
                                                                                                             gTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGc                           >  4:89650/1‑149 (MQ=255)
                                                                                                                         aTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACTCTGCTACCTGGCAAcc               >  1:130267/1‑149 (MQ=255)
                                                                                                                           gagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt             >  4:279147/1‑149 (MQ=255)
                                                                                                                           gagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt             >  4:107638/1‑149 (MQ=255)
                                                                                                                            agaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACTCTGCTACCTGGCAACCtt             <  2:130267/148‑1 (MQ=255)
                                                                                                                            agaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg            <  3:107638/149‑1 (MQ=255)
                                                                                                                             gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg           <  3:89650/149‑1 (MQ=255)
                                                                                                                              aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGGAACCTTgg           >  2:9219/1‑148 (MQ=255)
                                                                                                                              aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg           >  5:286411/1‑148 (MQ=255)
                                                                                                                               tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTggg          >  2:82403/1‑148 (MQ=255)
                                                                                                                                 gAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGC‑‑‑TGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAAc                >  7:179825/1‑137 (MQ=37)
                                                                                                                                 gAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGC‑‑‑TGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAAc                <  8:179825/137‑1 (MQ=37)
                                                                                                                                 gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGaa        <  1:9219/148‑1 (MQ=255)
                                                                                                                                      ccgcccgcGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATAGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGccc  >  5:355149/1‑149 (MQ=255)
                                                                                                                                            gcgcgGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACtctc                                            >  7:276022/1‑101 (MQ=255)
                                                                                                                                            gcgcgGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACtctc                                            <  8:276022/101‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCC  >  NC_002947/5313138‑5313420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑