Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,320,760 C→A 6.4% G52V (GGT→GTT)  PP_4682 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,320,7600CA 6.4% 200.0 / 3.7 62G52V (GGT→GTT) PP_4682TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (30/28);  new base A (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

ATCGGCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACAT  >  NC_002947/5320616‑5320904
                                                                                                                                                 |                                                                                                                                                
aTCGGCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACccgc                                                                                                                                              <  1:58361/149‑1 (MQ=255)
    gCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAg                                                                                                                                          <  6:304031/149‑1 (MQ=255)
     cGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGc                                                                                                                                         <  6:305681/149‑1 (MQ=255)
         gTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGtt                                                                                                                                     <  7:119696/149‑1 (MQ=255)
               aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                               >  1:76216/1‑149 (MQ=255)
               aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                               >  7:284038/1‑149 (MQ=255)
                  ggcgcggcgcCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCa                                                                                                                             <  4:325241/148‑1 (MQ=255)
                   gcgcggcgcCATTGGACGCCGGGAACGTCATGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCAc                                                                                                                            >  1:27367/1‑149 (MQ=255)
                            cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                 >  8:296199/1‑133 (MQ=255)
                            cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                 <  7:296199/134‑2 (MQ=255)
                             aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                 >  5:279067/1‑149 (MQ=255)
                             aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGCCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                 >  1:114144/1‑149 (MQ=255)
                              ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                <  2:114144/149‑1 (MQ=255)
                              ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                <  2:28602/149‑1 (MQ=255)
                              ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                <  4:306300/149‑1 (MQ=255)
                                              tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                 >  1:357623/1‑132 (MQ=255)
                                              tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                 <  2:357623/132‑1 (MQ=255)
                                                 tGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCg                                                                                               >  8:321694/1‑148 (MQ=255)
                                                   gATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAc                                                                                                                                                  >  5:138929/1‑95 (MQ=255)
                                                   gATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAc                                                                                                                                                  <  6:138929/95‑1 (MQ=255)
                                                     tGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCg                                                                                          <  2:27367/149‑1 (MQ=255)
                                                        ccGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGac                                                                                        >  5:117547/1‑148 (MQ=255)
                                                          gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                     >  5:182674/1‑149 (MQ=255)
                                                          gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                     <  6:117547/149‑1 (MQ=255)
                                                          gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                  <  2:154669/104‑1 (MQ=255)
                                                          gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                  >  1:154669/1‑104 (MQ=255)
                                                            aGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                   <  2:8860/149‑1 (MQ=255)
                                                                   gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                   >  8:156372/1‑142 (MQ=255)
                                                                   gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                   <  7:156372/142‑1 (MQ=255)
                                                                      cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                         <  6:182674/149‑1 (MQ=255)
                                                                      cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                         >  8:92359/1‑149 (MQ=255)
                                                                       ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                        >  2:24792/1‑149 (MQ=255)
                                                                       ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                        >  6:25973/1‑149 (MQ=255)
                                                                             ggATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCggg                                                                  <  2:76216/149‑1 (MQ=255)
                                                                                  atcaCGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACGACCGGAATCTTGCCCCGCGGGACGCt                                                             >  8:237916/1‑149 (MQ=255)
                                                                                        gTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCa                                                       >  8:211337/1‑149 (MQ=255)
                                                                                           acacCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCAccc                                                    <  2:130678/149‑1 (MQ=255)
                                                                                             acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                  >  1:76663/1‑149 (MQ=255)
                                                                                             acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                  <  5:25973/149‑1 (MQ=255)
                                                                                              ccGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTg                                                 >  6:126894/1‑149 (MQ=255)
                                                                                                      ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                            >  1:148886/1‑130 (MQ=255)
                                                                                                      ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                            <  2:148886/130‑1 (MQ=255)
                                                                                                      ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAg                                         >  6:10009/1‑149 (MQ=255)
                                                                                                      ctccGGCAGCGTCAGCCCCTGGGCGGTGCCGGGCTTATAGCCACCCGCCCAGCGGTTGGCCGTCACGCGTTCGCACTTGGACACGCGCCTGCTCGAATCGACCACCCGAATATTGCCACGCGGGACGCTGGAGCACGCCTCGAGCACAg                                         >  2:169645/1‑149 (MQ=255)
                                                                                                       tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                 >  8:62564/1‑124 (MQ=255)
                                                                                                       tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                 <  7:62564/124‑1 (MQ=255)
                                                                                                          ggCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCaa                                     >  4:25150/1‑149 (MQ=255)
                                                                                                            cAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACg                                   <  5:10009/149‑1 (MQ=255)
                                                                                                              gCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGcc                                 <  7:321694/149‑1 (MQ=255)
                                                                                                                    gcgcCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCCCCCCTGGAGCACAGCCAACGCCGTCACg                           >  3:271202/1‑149 (MQ=255)
                                                                                                                       ccTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCa                        <  7:92359/149‑1 (MQ=255)
                                                                                                                            gCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGaaa                   <  6:279067/149‑1 (MQ=255)
                                                                                                                                   cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                    <  6:187455/93‑1 (MQ=255)
                                                                                                                                   cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                    >  5:187455/1‑93 (MQ=255)
                                                                                                                                   cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGacac            >  2:294677/1‑149 (MQ=255)
                                                                                                                                    ggCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACt           >  5:264640/1‑149 (MQ=255)
                                                                                                                                     gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc            <  7:216520/147‑1 (MQ=255)
                                                                                                                                     gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc            >  8:216520/1‑147 (MQ=255)
                                                                                                                                     gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACtt          <  5:126894/149‑1 (MQ=255)
                                                                                                                                          ataGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtca     >  4:303223/1‑149 (MQ=255)
                                                                                                                                            aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt       >  2:185598/1‑145 (MQ=255)
                                                                                                                                            aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt       <  1:185598/145‑1 (MQ=255)
                                                                                                                                             gCCACCCGCCGAGCGGCTGGCCGTCCCGCGTGCGCTGTTGGACCCGCGGGTGCTCGACTCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACCGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat  <  4:271202/149‑1 (MQ=255)
                                                                                                                                              ccACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat  >  1:231496/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCGGCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACAT  >  NC_002947/5320616‑5320904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑