Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,320,766 G→C 7.8% S50W (TCG→TGG)  PP_4682 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,320,7660GC 7.8% 189.1 / 4.4 61S50W (TCG→TGG) PP_4682TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base C (3/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCAT  >  NC_002947/5320620‑5320914
                                                                                                                                                   |                                                                                                                                                    
gCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAg                                                                                                                                                    <  6:304031/149‑1 (MQ=255)
 cGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGc                                                                                                                                                   <  6:305681/149‑1 (MQ=255)
     gTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGtt                                                                                                                                               <  7:119696/149‑1 (MQ=255)
           aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                                         >  1:76216/1‑149 (MQ=255)
           aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                                         >  7:284038/1‑149 (MQ=255)
              ggcgcggcgcCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCa                                                                                                                                       <  4:325241/148‑1 (MQ=255)
               gcgcggcgcCATTGGACGCCGGGAACGTCATGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCAc                                                                                                                                      >  1:27367/1‑149 (MQ=255)
                        cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                           >  8:296199/1‑133 (MQ=255)
                        cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                           <  7:296199/134‑2 (MQ=255)
                         aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                           >  5:279067/1‑149 (MQ=255)
                         aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGCCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                           >  1:114144/1‑149 (MQ=255)
                          ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                          <  2:28602/149‑1 (MQ=255)
                          ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                          <  2:114144/149‑1 (MQ=255)
                          ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                          <  4:306300/149‑1 (MQ=255)
                                          tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                           <  2:357623/132‑1 (MQ=255)
                                          tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                           >  1:357623/1‑132 (MQ=255)
                                             tGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCg                                                                                                         >  8:321694/1‑148 (MQ=255)
                                                 tGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCg                                                                                                    <  2:27367/149‑1 (MQ=255)
                                                    ccGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGac                                                                                                  >  5:117547/1‑148 (MQ=255)
                                                      gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                               <  6:117547/149‑1 (MQ=255)
                                                      gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                               >  5:182674/1‑149 (MQ=255)
                                                      gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                            <  2:154669/104‑1 (MQ=255)
                                                      gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                            >  1:154669/1‑104 (MQ=255)
                                                        aGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                             <  2:8860/149‑1 (MQ=255)
                                                               gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                             <  7:156372/142‑1 (MQ=255)
                                                               gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                             >  8:156372/1‑142 (MQ=255)
                                                                  cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                                   >  8:92359/1‑149 (MQ=255)
                                                                  cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                                   <  6:182674/149‑1 (MQ=255)
                                                                   ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                                  >  2:24792/1‑149 (MQ=255)
                                                                   ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                                  >  6:25973/1‑149 (MQ=255)
                                                                         ggATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCggg                                                                            <  2:76216/149‑1 (MQ=255)
                                                                              atcaCGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACGACCGGAATCTTGCCCCGCGGGACGCt                                                                       >  8:237916/1‑149 (MQ=255)
                                                                                    gTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCa                                                                 >  8:211337/1‑149 (MQ=255)
                                                                                       acacCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCAccc                                                              <  2:130678/149‑1 (MQ=255)
                                                                                         acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                            >  1:76663/1‑149 (MQ=255)
                                                                                         acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                            <  5:25973/149‑1 (MQ=255)
                                                                                          ccGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTg                                                           >  6:126894/1‑149 (MQ=255)
                                                                                                  ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                                      >  1:148886/1‑130 (MQ=255)
                                                                                                  ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                                      <  2:148886/130‑1 (MQ=255)
                                                                                                  ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAg                                                   >  6:10009/1‑149 (MQ=255)
                                                                                                  ctccGGCAGCGTCAGCCCCTGGGCGGTGCCGGGCTTATAGCCACCCGCCCAGCGGTTGGCCGTCACGCGTTCGCACTTGGACACGCGCCTGCTCGAATCGACCACCCGAATATTGCCACGCGGGACGCTGGAGCACGCCTCGAGCACAg                                                   >  2:169645/1‑149 (MQ=255)
                                                                                                   tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                           >  8:62564/1‑124 (MQ=255)
                                                                                                   tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                           <  7:62564/124‑1 (MQ=255)
                                                                                                      ggCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCaa                                               >  4:25150/1‑149 (MQ=255)
                                                                                                        cAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACg                                             <  5:10009/149‑1 (MQ=255)
                                                                                                          gCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGcc                                           <  7:321694/149‑1 (MQ=255)
                                                                                                                gcgcCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCCCCCCTGGAGCACAGCCAACGCCGTCACg                                     >  3:271202/1‑149 (MQ=255)
                                                                                                                   ccTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCa                                  <  7:92359/149‑1 (MQ=255)
                                                                                                                        gCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGaaa                             <  6:279067/149‑1 (MQ=255)
                                                                                                                               cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                              >  5:187455/1‑93 (MQ=255)
                                                                                                                               cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                              <  6:187455/93‑1 (MQ=255)
                                                                                                                               cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGacac                      >  2:294677/1‑149 (MQ=255)
                                                                                                                                ggCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACt                     >  5:264640/1‑149 (MQ=255)
                                                                                                                                 gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc                      <  7:216520/147‑1 (MQ=255)
                                                                                                                                 gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc                      >  8:216520/1‑147 (MQ=255)
                                                                                                                                 gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACtt                    <  5:126894/149‑1 (MQ=255)
                                                                                                                                      ataGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtca               >  4:303223/1‑149 (MQ=255)
                                                                                                                                        aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt                 >  2:185598/1‑145 (MQ=255)
                                                                                                                                        aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt                 <  1:185598/145‑1 (MQ=255)
                                                                                                                                         gCCACCCGCCGAGCGGCTGGCCGTCCCGCGTGCGCTGTTGGACCCGCGGGTGCTCGACTCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACCGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat            <  4:271202/149‑1 (MQ=255)
                                                                                                                                          ccACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat            >  1:231496/1‑148 (MQ=255)
                                                                                                                                               caccgAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACAcc      >  6:83309/3‑149 (MQ=255)
                                                                                                                                                   gAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCtcat  <  1:24792/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGAGGTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCAT  >  NC_002947/5320620‑5320914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑