Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,320,772 T→G 6.7% N48T (AAC→ACC)  PP_4682 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,320,7720TG 6.7% 187.6 / 4.5 62N48T (AAC→ACC) PP_4682TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (32/25);  new base G (2/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

GTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCATCAATG  >  NC_002947/5320625‑5320919
                                                                                                                                                    |                                                                                                                                                   
gTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGtt                                                                                                                                                    <  7:119696/149‑1 (MQ=255)
      aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                                              >  1:76216/1‑149 (MQ=255)
      aTCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGt                                                                                                                                              >  7:284038/1‑149 (MQ=255)
         ggcgcggcgcCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCa                                                                                                                                            <  4:325241/148‑1 (MQ=255)
          gcgcggcgcCATTGGACGCCGGGAACGTCATGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCAc                                                                                                                                           >  1:27367/1‑149 (MQ=255)
                   cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                                >  8:296199/1‑133 (MQ=255)
                   cATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGct                                                                                                                                                <  7:296199/134‑2 (MQ=255)
                    aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                                >  5:279067/1‑149 (MQ=255)
                    aTTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGCCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                                >  1:114144/1‑149 (MQ=255)
                     ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                               <  2:28602/149‑1 (MQ=255)
                     ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                               <  4:306300/149‑1 (MQ=255)
                     ttGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGtt                                                                                                                               <  2:114144/149‑1 (MQ=255)
                                     tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                                >  1:357623/1‑132 (MQ=255)
                                     tC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGt                                                                                                                                <  2:357623/132‑1 (MQ=255)
                                        tGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCg                                                                                                              >  8:321694/1‑148 (MQ=255)
                                            tGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCg                                                                                                         <  2:27367/149‑1 (MQ=255)
                                               ccGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGac                                                                                                       >  5:117547/1‑148 (MQ=255)
                                                 gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                                    <  6:117547/149‑1 (MQ=255)
                                                 gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGaccac                                                                                                    >  5:182674/1‑149 (MQ=255)
                                                 gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                                 >  1:154669/1‑104 (MQ=255)
                                                 gAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCAACCGCTCGGCGGGTGGc                                                                                                                                                 <  2:154669/104‑1 (MQ=255)
                                                   aGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                                  <  2:8860/149‑1 (MQ=255)
                                                          gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                                  >  8:156372/1‑142 (MQ=255)
                                                          gcgcCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCg                                                                                                  <  7:156372/142‑1 (MQ=255)
                                                             cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                                        <  6:182674/149‑1 (MQ=255)
                                                             cccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGcc                                                                                        >  8:92359/1‑149 (MQ=255)
                                                              ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                                       >  2:24792/1‑149 (MQ=255)
                                                              ccTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCa                                                                                       >  6:25973/1‑149 (MQ=255)
                                                                    ggATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCggg                                                                                 <  2:76216/149‑1 (MQ=255)
                                                                         atcaCGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACGACCGGAATCTTGCCCCGCGGGACGCt                                                                            >  8:237916/1‑149 (MQ=255)
                                                                               gTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCa                                                                      >  8:211337/1‑149 (MQ=255)
                                                                                  acacCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCAccc                                                                   <  2:130678/149‑1 (MQ=255)
                                                                                    acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                                 >  1:76663/1‑149 (MQ=255)
                                                                                    acCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCt                                                                 <  5:25973/149‑1 (MQ=255)
                                                                                     ccGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTg                                                                >  6:126894/1‑149 (MQ=255)
                                                                                             ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                                           >  1:148886/1‑130 (MQ=255)
                                                                                             ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTg                                                                           <  2:148886/130‑1 (MQ=255)
                                                                                             ctccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAg                                                        >  6:10009/1‑149 (MQ=255)
                                                                                             ctccGGCAGCGTCAGCCCCTGGGCGGTGCCGGGCTTATAGCCACCCGCCCAGCGGTTGGCCGTCACGCGTTCGCACTTGGACACGCGCCTGCTCGAATCGACCACCCGAATATTGCCACGCGGGACGCTGGAGCACGCCTCGAGCACAg                                                        >  2:169645/1‑149 (MQ=255)
                                                                                              tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                                <  7:62564/124‑1 (MQ=255)
                                                                                              tccGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGa                                                                                >  8:62564/1‑124 (MQ=255)
                                                                                                 ggCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCaa                                                    >  4:25150/1‑149 (MQ=255)
                                                                                                   cAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACg                                                  <  5:10009/149‑1 (MQ=255)
                                                                                                     gCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGcc                                                <  7:321694/149‑1 (MQ=255)
                                                                                                           gcgcCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCCCCCCTGGAGCACAGCCAACGCCGTCACg                                          >  3:271202/1‑149 (MQ=255)
                                                                                                              ccTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCa                                       <  7:92359/149‑1 (MQ=255)
                                                                                                                   gCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGaaa                                  <  6:279067/149‑1 (MQ=255)
                                                                                                                          cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                                   <  6:187455/93‑1 (MQ=255)
                                                                                                                          cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCAcgcg                                                                                   >  5:187455/1‑93 (MQ=255)
                                                                                                                          cGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGacac                           >  2:294677/1‑149 (MQ=255)
                                                                                                                           ggCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACt                          >  5:264640/1‑149 (MQ=255)
                                                                                                                            gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc                           >  8:216520/1‑147 (MQ=255)
                                                                                                                            gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCAc                           <  7:216520/147‑1 (MQ=255)
                                                                                                                            gCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACtt                         <  5:126894/149‑1 (MQ=255)
                                                                                                                                 ataGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtca                    >  4:303223/1‑149 (MQ=255)
                                                                                                                                   aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt                      >  2:185598/1‑145 (MQ=255)
                                                                                                                                   aGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGtt                      <  1:185598/145‑1 (MQ=255)
                                                                                                                                    gCCACCCGCCGAGCGGCTGGCCGTCCCGCGTGCGCTGTTGGACCCGCGGGTGCTCGACTCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACCGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat                 <  4:271202/149‑1 (MQ=255)
                                                                                                                                     ccACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTtcacat                 >  1:231496/1‑148 (MQ=255)
                                                                                                                                          caccgAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACAcc           >  6:83309/3‑149 (MQ=255)
                                                                                                                                              gAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCtcat       <  1:24792/149‑1 (MQ=255)
                                                                                                                                                   gTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCATCAATg  <  2:56833/149‑1 (MQ=255)
                                                                                                                                                   gTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCATCAATg  >  3:15443/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTACTGATCGGCGCGGCGCCATTGGACGCCGGGAACGTC‑TGGATGCCCGAAGCGCCTGCGCCCTGCGGGATCATCACGGTGACACCGGAATCCTCCGGCAGCGTCTGCGCCTGGGCGGTGCCGGCGTTATAGCCACCCGCCGAGCGGTTGGCCGTCACGCGTTCGCTGTTGGACACGCGGGTGCTCGAATCGACCACCGGAATATTGCCACGCGGGACGCTGGAGCACCCCTGGAGCACAGCCAACGCCGTCACGGCAGGAAACAGCCACTTGTTCACATCACACCTCATCAATG  >  NC_002947/5320625‑5320919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑