Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,053 C→G 9.6% intergenic (+60/‑224) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,0530CG 9.6% 145.9 / 5.5 51intergenic (+60/‑224)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base G (3/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

GCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337914‑5338199
                                                                                                                                           |                                                                                                                                                   
gCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                            >  6:170820/1‑149 (MQ=255)
  gTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCgg                                                                                                                                          <  3:169550/149‑1 (MQ=255)
   tAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCggt                                                                                                                                         <  2:41935/149‑2 (MQ=255)
                ccTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCt                                                                                                                             >  5:323485/1‑148 (MQ=255)
                 cTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGCGCGCCGCCCGCGCGGCGCTCGGACTCACAGGCGCTg                                                                                                                            >  8:15396/1‑148 (MQ=255)
                 cTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATCCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGa                                                                                                                           <  5:351495/149‑1 (MQ=255)
                  tgtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTCCGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGaa                                                                                                                          >  5:281224/1‑149 (MQ=255)
                   gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                           <  5:254488/131‑1 (MQ=255)
                   gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                           >  6:254488/1‑131 (MQ=255)
                            ccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑acga                                                                                                               <  6:261713/149‑1 (MQ=255)
                             cgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCTAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑acgac                                                                                                              <  1:177576/149‑1 (MQ=255)
                                   cgcCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑acgacgttacc                                                                                                        <  5:170820/149‑6 (MQ=255)
                                          gCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCAGGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGt                                                                                                 <  8:158043/149‑1 (MQ=255)
                                               ccACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAacac                                                                                            <  6:323485/149‑1 (MQ=255)
                                                cACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGCACACCCCAGGTAacac                                                                                            >  7:242188/1‑148 (MQ=255)
                                                 aCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAAcacct                                                                                          >  3:218105/1‑149 (MQ=255)
                                                         agagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  7:168500/149‑1 (MQ=255)
                                                          gagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCg                                                                                 >  6:164272/1‑149 (MQ=255)
                                                              aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaa                                                                             <  4:218105/149‑1 (MQ=255)
                                                                    aCCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTg                                                                       >  7:101351/1‑149 (MQ=255)
                                                                     ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  3:78861/147‑1 (MQ=255)
                                                                     ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                      <  8:101351/149‑1 (MQ=255)
                                                                        gCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   >  6:88941/1‑149 (MQ=255)
                                                                        gCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   >  2:26559/1‑149 (MQ=255)
                                                                           aaTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  5:353320/141‑1 (MQ=255)
                                                                           aaTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        >  6:353320/1‑141 (MQ=255)
                                                                           aaTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAccc                                                                 >  6:103712/1‑148 (MQ=255)
                                                                                cccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCTACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  1:224881/127‑1 (MQ=255)
                                                                                cccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  >  2:224881/1‑126 (MQ=255)
                                                                                   aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTcc                                                        <  5:103712/149‑1 (MQ=255)
                                                                                        cgcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACAGCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgccg                                                    >  7:55657/1‑148 (MQ=255)
                                                                                        cgcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgccg                                                    >  2:276644/1‑148 (MQ=255)
                                                                                        cgcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGt                                                   <  7:15396/149‑1 (MQ=255)
                                                                                          caccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAAcacctca                                                                                        >  4:335169/1‑110 (MQ=255)
                                                                                          caccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAAcacctca                                                                                        <  3:335169/110‑1 (MQ=255)
                                                                                               cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                            <  5:65814/149‑1 (MQ=255)
                                                                                                  gccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcccc                                         >  6:102677/1‑149 (MQ=255)
                                                                                                  gccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACCTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcccc                                         >  4:65902/1‑149 (MQ=255)
                                                                                                    cgcACCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACCAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                       <  6:281224/149‑1 (MQ=255)
                                                                                                          cTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTa                                 >  3:21182/1‑149 (MQ=255)
                                                                                                           tATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTAc                                >  3:261598/1‑149 (MQ=255)
                                                                                                             tGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                            >  5:331799/1‑135 (MQ=255)
                                                                                                             tGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                            <  6:331799/135‑1 (MQ=255)
                                                                                                                      ggTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGaaa                      <  5:88941/148‑1 (MQ=255)
                                                                                                                       gTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAAtt                    <  5:102677/149‑1 (MQ=255)
                                                                                                                        tGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGt                                                                                >  4:245297/1‑88 (MQ=255)
                                                                                                                        tGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGt                                                                                <  3:245297/88‑1 (MQ=255)
                                                                                                                            gccGAGCGCCGCGCGGGCGGCGCTCGGTCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                >  5:64718/2‑148 (MQ=255)
                                                                                                                            aCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                <  8:242188/148‑1 (MQ=255)
                                                                                                                               gAGCGCCGCGCGGGCGGCGCTCGGTCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtt            >  2:264616/1‑149 (MQ=255)
                                                                                                                                 gcgcCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtttt          <  3:65902/149‑1 (MQ=255)
                                                                                                                                     cgcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCTGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtttt          >  4:124833/1‑145 (MQ=255)
                                                                                                                                     cgcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCTGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtttt          <  3:124833/145‑1 (MQ=255)
                                                                                                                                      gcccgcGCGCCGCTCGGCCTCACAGGCGCTGACCGTGTC‑ACGACCAACACCCCAGGTAACACCTCACCTCCCGTAAAACTCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAAcc     <  4:261598/149‑1 (MQ=255)
                                                                                                                                         cgcgcgGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAAGAACTCACCTCCCGTATAAATCTGTTACCCAAGCTTTCCGCGGTATCAGTCACCGAGGCGTAAGGTTACGAAATTGCGTCATTTTGaaggtgc  >  4:276099/1‑144 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTC‑ACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337914‑5338199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑