Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,350,085	C→G	7.9%	A853A (GCC→GCG) ‡	PP_4704 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,350,085	0	C	G	7.9%	191.2 / 6.4	63	A853A (GCC→GCG) ‡	PP_4704	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base G (3/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGT  >  NC_002947/5349939‑5350233                                                                                                                                                   |                                                                                                                                                      aGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCACCAGCGCCTTGAGCGTGAGTACCAGGCCAc                                                                                                                                                     >  2:112155/1‑149 (MQ=255)           cccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActca                                                                                                                                           >  7:70241/1‑146 (MQ=255)            ccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActcac                                                                                                                                          <  8:70241/149‑5 (MQ=255)              aGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActcacgc                                                                                                                                        <  6:236377/149‑7 (MQ=255)                   tcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCACCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCa                                                                                                                                   <  8:252525/149‑1 (MQ=255)                     cTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGc                                                                                                                                 >  7:105145/1‑149 (MQ=255)                           cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACggg                                                                                                                           <  8:105145/149‑1 (MQ=255)                            aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACggg‑g                                                                                                                         <  3:352229/149‑1 (MQ=255)                              gCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑Gt                                                                                                                        >  4:287025/1‑148 (MQ=255)                                tGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCAACGGG‑GTGa                                                                                                                      >  5:313864/1‑148 (MQ=255)                                  gCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGAc                                                                                                                     <  5:342503/147‑1 (MQ=255)                                   cATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAg                                                                                                                  <  3:287025/149‑1 (MQ=255)                                     tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGCGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGt                                                                                                                >  1:187576/1‑149 (MQ=255)                                       cgcgCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGc                                                                                                              >  4:234086/1‑149 (MQ=255)                                               aaGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCCACGGG‑GTGACAAGGTGCCGGCCAAt                                                                                                      <  6:313864/149‑1 (MQ=255)                                                   gcgcCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCt                                                                                                  <  8:148663/149‑1 (MQ=255)                                                     gcCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTc                                                                                                 >  5:268782/1‑148 (MQ=255)                                                            aCTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtgg                                                                                         <  2:187576/149‑1 (MQ=255)                                                                cgcCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtggtggt                                                                                     <  1:272387/149‑1 (MQ=255)                                                                  ccTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtggtggtg                                                                                    >  3:197703/1‑148 (MQ=255)                                                                  ccTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑CTGACAAGGTGCCGGCCAATGCCTCGATGCtggtggtgg                                                                                   >  2:135929/1‑149 (MQ=255)                                                                   cTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGAAAGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGga                                                                                  >  1:297675/1‑149 (MQ=255)                                                                     ggAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgg                                                                                                                            >  2:182855/1‑106 (MQ=255)                                                                     ggAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgg                                                                                                                            <  1:182855/106‑1 (MQ=255)                                                                     ggAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGgac                                                                                 >  5:286901/1‑148 (MQ=255)                                                                         aTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtg                                                                                          <  4:296300/135‑1 (MQ=255)                                                                         aTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtg                                                                                          >  3:296300/1‑135 (MQ=255)                                                                         aTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAg                                                                            <  6:286901/149‑1 (MQ=255)                                                                                  aCCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGAc                                                                        <  1:284814/144‑1 (MQ=255)                                                                                  aCCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGAc                                                                        >  2:284814/1‑144 (MQ=255)                                                                                   ccAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCaa                                                                                                       >  1:306699/1‑112 (MQ=255)                                                                                   ccAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCaa                                                                                                       <  2:306699/112‑1 (MQ=255)                                                                                      gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCcg                                                                 <  3:159427/147‑1 (MQ=255)                                                                                      gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCcg                                                                 >  4:159427/1‑147 (MQ=255)                                                                                           cAGGGCACCGTCGGGCCGCTGATTGCCCAACAGCGCCTTGAGCGTTATTTCCACGGCACCTTGTAAAATATCCTGCATCGTCGGGTGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGc                                                           <  1:106800/149‑1 (MQ=255)                                                                                               ccAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑CTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGaa                                                      <  1:135929/149‑1 (MQ=255)                                                                                                 gtcgctggCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAgg                                                    <  1:12861/143‑1 (MQ=255)                                                                                                    gACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAt                                                 <  2:297675/149‑1 (MQ=255)                                                                                                     aCGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAtt                                                >  2:176238/1‑149 (MQ=255)                                                                                                                    cccAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTAcc                                            >  3:265938/1‑138 (MQ=255)                                                                                                                    cccAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTAcc                                            <  4:265938/138‑1 (MQ=255)                                                                                                                     ccAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTCACCGTACCCGCCTg                                 >  8:43274/1‑148 (MQ=255)                                                                                                                      cAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGa                                                                         <  6:161007/107‑1 (MQ=255)                                                                                                                      cAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGa                                                                         >  5:161007/1‑107 (MQ=255)                                                                                                                       aaCAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGaaa                              <  6:268782/149‑1 (MQ=255)                                                                                                                        aCAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGaaa                              >  6:99179/1‑148 (MQ=255)                                                                                                                           gcgcCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTTTATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAAcagc                          <  1:112155/149‑1 (MQ=255)                                                                                                                            cgcCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAAcagca                         <  5:99179/149‑1 (MQ=255)                                                                                                                               cTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgcc                      >  5:102305/1‑149 (MQ=255)                                                                                                                                      gTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGcc               >  5:146704/1‑149 (MQ=255)                                                                                                                                         tgTACCAGGTGGCCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGaa            >  4:170463/2‑149 (MQ=255)                                                                                                                                          gTACCAGGTGGCCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAt           >  1:225603/1‑149 (MQ=255)                                                                                                                                          gTACCAGGCCACCTGGTACAAGCTCCTGCAGCGACGGG‑GTGACAACGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAt           <  8:260976/149‑1 (MQ=255)                                                                                                                                          gTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGaaa                              <  4:227441/130‑1 (MQ=255)                                                                                                                                          gTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGaaa                              >  3:227441/1‑130 (MQ=255)                                                                                                                                          gTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAt           <  6:146704/149‑1 (MQ=255)                                                                                                                                           tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATg          <  1:176238/149‑1 (MQ=255)                                                                                                                                           tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATg          >  1:236915/1‑149 (MQ=255)                                                                                                                                             ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATgg         <  4:197703/148‑1 (MQ=255)                                                                                                                                               aGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAg      <  2:236915/149‑1 (MQ=255)                                                                                                                                                ggCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTCCCGTCCCCGCCTGAACCAGCAGCCGCCAGCCGAATGGGAg      >  7:312541/1‑148 (MQ=255)                                                                                                                                                 gCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtggtggtgg                                                                                   >  3:108577/1‑70 (MQ=255)                                                                                                                                                 gCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCtggtggtgg                                                                                   <  4:108577/70‑1 (MQ=255)                                                                                                                                                   cACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGt  >  4:160582/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGG‑GTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGT  >  NC_002947/5349939‑5350233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑