| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,351,342 | A→C | 7.4% | intergenic (+21/+55) | PP_4706 → / ← PP_4707 | hypothetical protein/OsmY‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,351,342 | 0 | A | C | 7.4% | 157.6 / 4.9 | 54 | intergenic (+21/+55) | PP_4706/PP_4707 | hypothetical protein/OsmY‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (23/27); new base C (2/2); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
ATGCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGT > NC_002947/5351197‑5351486 | atgCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACgg < 1:16287/149‑1 (MQ=255) ggcgTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCa < 8:82675/149‑1 (MQ=255) gTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAggg > 4:211998/1‑149 (MQ=255) tGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGTGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCtt < 4:28163/149‑1 (MQ=255) tGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCtt < 3:211998/149‑1 (MQ=255) aTACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGaaa < 1:44864/149‑1 (MQ=255) cAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGc > 3:165537/1‑149 (MQ=255) cATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGATGAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAACCCCTGGCACGGCCCAGGGCTTCGATGAAAGTGCCCAAATc > 1:282275/1‑148 (MQ=255) tCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGc > 5:131860/1‑149 (MQ=255) gcgACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGcc < 5:172727/148‑1 (MQ=255) gACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGAc > 3:349995/1‑149 (MQ=255) aGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACggg < 7:237735/149‑1 (MQ=255) cGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTg < 5:268742/123‑1 (MQ=255) cGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTg > 6:268742/1‑123 (MQ=255) gTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACAt > 7:185199/1‑149 (MQ=255) ctttGGTGGAGCCTCTGTTCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTcc < 2:282275/147‑1 (MQ=255) cATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTcc < 8:324125/149‑1 (MQ=255) cATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTcc < 8:185199/149‑1 (MQ=255) aTTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGAACATTCCt > 6:132851/1‑149 (MQ=255) gAGCCTCTGATCCTTGAGCAGGAAAGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCgg > 5:213552/1‑149 (MQ=255) tGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAg > 2:297724/1‑149 (MQ=255) aTCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGa > 1:56229/1‑121 (MQ=255) aTCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGa < 2:56229/121‑1 (MQ=255) cTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAg < 6:84587/83‑1 (MQ=255) cTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAg > 5:84587/1‑83 (MQ=255) cTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGt < 4:165537/149‑1 (MQ=255) gAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTc < 3:275168/133‑1 (MQ=255) gAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTc > 4:275168/1‑133 (MQ=255) aaTGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTTAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTAc < 1:297724/149‑1 (MQ=255) cTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGct > 8:267044/1‑148 (MQ=255) cTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGct < 7:267044/148‑1 (MQ=255) ttCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACggg > 5:111140/1‑107 (MQ=255) ttCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACggg < 6:111140/107‑1 (MQ=255) cGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt < 3:160113/148‑1 (MQ=255) cgcgcgCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTc > 6:32519/1‑149 (MQ=255) cgcgcAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCtt < 6:15506/149‑1 (MQ=255) gcgcAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg > 6:79600/1‑149 (MQ=255) gcgcAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg < 4:349995/149‑1 (MQ=255) cccGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATg > 7:325557/1‑149 (MQ=255) ccGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAGGCCTTTGATGTTCTTGGTAATgc > 8:236488/1‑149 (MQ=255) gtgtATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCa < 5:32519/149‑1 (MQ=255) gtgtATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGAGGAAAGTGGCCAAATCGGTCGGGCCGGCGGGACATTCCTTATTCGGCTTTCAGGCCGGCGGCGATTACGCTCTGGACGGGGTGGATGTTCTTGGTAATgcgga > 4:55941/1‑147 (MQ=255) aTTGAGCGCAAGAATAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAc < 4:130146/119‑1 (MQ=255) aTTGAGCGCAAGAAGAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAc > 3:130146/1‑119 (MQ=255) ttGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCt > 4:318217/1‑149 (MQ=255) aGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCt < 6:131860/149‑1 (MQ=255) cgcAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACAtt < 8:238558/102‑1 (MQ=255) cgcAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACAtt > 7:238558/1‑102 (MQ=255) aaGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCttt < 6:213552/149‑1 (MQ=255) aaaaGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGAACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTg < 5:132851/148‑1 (MQ=255) aaGCCCTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGa > 3:179437/1‑149 (MQ=255) gCCCTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTc < 4:121619/84‑1 (MQ=255) gCCCTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTc > 3:121619/1‑84 (MQ=255) tGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGt < 4:179437/149‑1 (MQ=255) | ATGCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGA‑‑AAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGT > NC_002947/5351197‑5351486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |