Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,364,380	A→G	9.1%	V85A (GTG→GCG)	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,364,380	0	A	G	9.1%	114.9 / 2.9	45	V85A (GTG→GCG)	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base G (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TCCAGGGCGCCATCGCGCTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCG  >  NC_002947/5364233‑5364526                                                                                                                                                    |                                                                                                                                                   tCCAGGGCGCCATCGCGCTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCcac                                                                                                                                                   >  6:239714/1‑149 (MQ=255)  ccAGGGCGCCATCGCGCTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCcaca                                                                                                                                                  >  4:198598/1‑149 (MQ=255)       gcgcCATCGCGCTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGACGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTc                                                                                                                                             >  8:235776/1‑149 (MQ=255)                 gcTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCg                                                                                                                                   <  3:262171/149‑1 (MQ=255)                        ggCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTcc                                                                                                                                            >  4:61735/1‑133 (MQ=255)                        ggCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTcc                                                                                                                                            <  3:61735/133‑1 (MQ=255)                          cccGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCgag                                                                                                                          >  6:120333/1‑149 (MQ=255)                            cGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCgaga                                                                                                                         >  2:280791/1‑148 (MQ=255)                                   cgtTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCAcgc                                                                                                                 <  4:219145/149‑1 (MQ=255)                                    gtTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCAcgcg                                                                                                                >  1:119762/1‑149 (MQ=255)                                    gtTGATCAGCCCGGACCGCAGGCGCGCGGCTTCACGCATGAGAGCAGGCGTGGAGGTTTCGACGGATATGACGTGGTGGTGGCGACTGTTGATGGCCTCGACCATTTGCGCCTCACGCTCCAGCTCCTCGGTCACCga                                                                                                                           <  3:161304/138‑1 (MQ=255)                                         tCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCAccc                                                                                                           <  5:239714/149‑1 (MQ=255)                                             cccGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGgc                                                                                                       >  5:225077/1‑149 (MQ=255)                                              ccGGCCCCCAGGCGCGCGGCTTCACGCATGACAACAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGgcg                                                                                                      >  5:140686/1‑149 (MQ=255)                                                      cAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACt                                                                                              >  4:287868/1‑149 (MQ=255)                                                       aGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTc                                                                                             <  7:255158/149‑1 (MQ=255)                                                       aGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGCCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTc                                                                                             <  7:235776/149‑1 (MQ=255)                                                         gagcgcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTc                                                                                             <  8:97252/145‑1 (MQ=255)                                                            cgcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCAc                                                                                        <  3:287868/149‑1 (MQ=255)                                                             gcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCAcc                                                                                       >  2:194740/1‑149 (MQ=255)                                                               ggCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGa                                                                                     <  1:88939/149‑1 (MQ=255)                                                                       gCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGGCCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGAt                                                                              >  1:152751/1‑148 (MQ=255)                                                                       gCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATc                                                                             <  5:120333/149‑1 (MQ=255)                                                                         aTGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCAACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGAGTGGACTCGCCACCGATATCGATCaa                                                                           >  7:14083/1‑149 (MQ=255)                                                                                       ggAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGtt                                                                       >  4:146392/1‑139 (MQ=255)                                                                                       ggAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGtt                                                                       <  3:146392/139‑1 (MQ=255)                                                                                        gAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCggcggc                                                            <  1:280791/149‑1 (MQ=255)                                                                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCGCACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggcg                                                              <  5:271279/139‑1 (MQ=255)                                                                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCGCACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggcg                                                              <  5:276152/139‑1 (MQ=255)                                                                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCGCACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggcg                                                              >  6:271279/1‑139 (MQ=255)                                                                                                gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCGCACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcggcg                                                              >  6:276152/1‑139 (MQ=255)                                                                                                  ccGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCt                                                  >  1:241811/1‑149 (MQ=255)                                                                                                    gAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGGCCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTc                                                <  2:152751/149‑1 (MQ=255)                                                                                                    gAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTc                                                >  4:314907/1‑149 (MQ=255)                                                                                                     aaaTGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCg                                               >  5:197435/1‑149 (MQ=255)                                                                                                     aaaTGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCg                                               <  3:198598/149‑1 (MQ=255)                                                                                                               gTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCa                                     <  6:140686/149‑1 (MQ=255)                                                                                                                            ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgcg                        >  6:265181/1‑149 (MQ=255)                                                                                                                            ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGAGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgcg                        >  8:188844/1‑149 (MQ=255)                                                                                                                                tGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                    <  2:119762/149‑1 (MQ=255)                                                                                                                                    cTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgactg                 >  5:170603/1‑148 (MQ=255)                                                                                                                                     tCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgactg                 >  7:98891/1‑147 (MQ=255)                                                                                                                                          cATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc          <  6:197435/149‑1 (MQ=255)                                                                                                                                               gcgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCa     <  4:187306/149‑1 (MQ=255)                                                                                                                                                 gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCggcggc                                                            <  8:13666/92‑1 (MQ=255)                                                                                                                                                 gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCggcggc                                                            >  7:13666/1‑92 (MQ=255)                                                                                                                                                  ccACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg  <  8:98891/149‑1 (MQ=255)                                                                                                                                                   caccCGCTCCATCTCCTCGGTCACCGAGACTGCACGCGCACCCGTGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                    <  8:42493/130‑1 (MQ=255)                                                                                                                                                   cacaCGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                    >  7:42493/1‑130 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCCAGGGCGCCATCGCGCTCCAGGGCCCGCACGTCGTTGATCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCG  >  NC_002947/5364233‑5364526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑