Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,583	C→A	6.4%	intergenic (+672/‑50)	PP_4738 → / → PP_mr56	hypothetical protein/C4 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,583	0	C	A	6.4%	193.6 / 3.8	63	intergenic (+672/‑50)	PP_4738/PP_mr56	hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (26/32);  new base A (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

GCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGA  >  NC_002947/5390435‑5390729                                                                                                                                                     |                                                                                                                                                    gCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGc                                                                                                                                                     <  1:275245/149‑1 (MQ=255)   gTCTTTTTTTGTGCCTGGAATTTGGTCGCTGCGGGTCTAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtc                                                                                                                                                   <  6:183172/149‑1 (MQ=255)      tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                 >  8:145989/1‑148 (MQ=255)      tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                 >  2:71503/1‑148 (MQ=255)          tttGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAAc                                                                                                                                             <  2:296192/148‑1 (MQ=255)              tgCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGCTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAccc                                                                                                                                        <  7:321026/149‑2 (MQ=255)                ccTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                      <  7:145989/149‑1 (MQ=255)                ccTGGAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                      >  5:235650/1‑149 (MQ=255)                  tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCtt                                                                                                                                    >  7:280311/1‑149 (MQ=255)                    gAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                   <  6:235650/148‑1 (MQ=255)                     aaTTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGa                                                                                                                                 <  3:203802/149‑1 (MQ=255)                          gggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                            >  2:235395/1‑149 (MQ=255)                           ggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                            <  2:6925/148‑1 (MQ=255)                                  gggggCGAAAAATCGGTTTTAACCCCTTTCTTGTAGGGCGTTTCCCTAAGTTACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCTGTGGGTCGGTGGTCATGAACGACCGGCTTTTACAGGCCGCAACGa                                                                                                                    <  8:6133/148‑1 (MQ=255)                                  cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAAc                                                                                                                      <  2:27298/147‑1 (MQ=255)                                  cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGa                                                                                                                    <  5:6000/149‑1 (MQ=255)                                             aTCGGATTTAACCCCTTTCTTGTAGGGCGTTTCGCAAAGATACTGCCTGCTGCTGTTTTCCGTTGCGGTCATTTTTTCATGTTTTTAGTCTCTNTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgcgcg                                                                                                        <  5:3443/149‑1 (MQ=255)                                              tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                           <  7:133309/145‑1 (MQ=255)                                              tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                           >  8:133309/1‑145 (MQ=255)                                                      aaCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTCTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGt                                                                                               >  2:342119/1‑149 (MQ=255)                                                          ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  4:212333/1‑149 (MQ=255)                                                          ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  1:287026/1‑149 (MQ=255)                                                          ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTAATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  1:171038/1‑149 (MQ=255)                                                            ttacTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCTTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATgg                                                                                         >  5:278879/4‑149 (MQ=255)                                                               cTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGGTTTAGTCTCGGCGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCg                                                                                       <  6:20918/148‑1 (MQ=255)                                                                   tAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgt                                                                                  <  1:235395/149‑1 (MQ=255)                                                                    ggcgCGTTTCCCAACGATACTGCCTGCGCCTGTTTTCCGGTGCGGTCATTTTTTCAGGTTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtg                                                                                   <  8:288430/144‑1 (MQ=255)                                                                    aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgtg                                                                                 >  4:148229/1‑149 (MQ=255)                                                                    aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgta                                                                                 >  7:288430/1‑148 (MQ=255)                                                                      ggCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCa                                                                               <  1:71503/149‑1 (MQ=255)                                                                         gTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATgg                                                                            <  8:280311/149‑1 (MQ=255)                                                                              ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  4:90506/1‑149 (MQ=255)                                                                              ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  7:253383/1‑149 (MQ=255)                                                                              ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  5:36425/1‑149 (MQ=255)                                                                                aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                 <  5:38505/121‑1 (MQ=255)                                                                                aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                 >  6:38505/1‑121 (MQ=255)                                                                                aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGc                                                                     >  5:105206/1‑149 (MQ=255)                                                                                        tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                   >  4:110183/1‑143 (MQ=255)                                                                                        tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                   <  3:110183/143‑1 (MQ=255)                                                                                           ctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGcc                                                          <  2:287026/149‑1 (MQ=255)                                                                                              cgcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGt                                                       <  2:219814/149‑1 (MQ=255)                                                                                                ccTGTTTTCCGGTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGGCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGttt                                                     <  4:131297/149‑1 (MQ=255)                                                                                                   gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   >  8:284225/1‑148 (MQ=255)                                                                                                   gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  7:284225/148‑1 (MQ=255)                                                                                                    ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgt                                                 <  6:36425/149‑1 (MQ=255)                                                                                                    ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  8:253383/147‑1 (MQ=255)                                                                                                    ttgtCCGCTGCGGTCATTTATTCAGTGGTTTAGTCTCTGTGCGTCGTTGCTCATCAACGACCGGCTTTGACAGCCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  6:278879/144‑1 (MQ=255)                                                                                                         cGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCg                                            >  7:158511/1‑149 (MQ=255)                                                                                                           ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                         <  3:37400/134‑1 (MQ=255)                                                                                                           ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                         >  4:37400/1‑134 (MQ=255)                                                                                                             gCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                         <  3:148229/148‑1 (MQ=255)                                                                                                              cGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGt                                       <  3:212333/149‑1 (MQ=255)                                                                                                                 tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTg                                    >  2:217380/1‑149 (MQ=255)                                                                                                                   aTTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGAACGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                   <  8:158511/148‑1 (MQ=255)                                                                                                                     tttttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                                >  8:110295/1‑149 (MQ=255)                                                                                                                           aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑AACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGGCAACCCATg                          >  1:282321/1‑149 (MQ=255)                                                                                                                           aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          <  6:105206/149‑1 (MQ=255)                                                                                                                                         ggTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca               <  1:342119/146‑1 (MQ=255)                                                                                                                                           tGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCTCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAtt          <  2:171038/149‑1 (MQ=255)                                                                                                                                           ccggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGAGTACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAt           >  1:74985/3‑149 (MQ=255)                                                                                                                                            cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          <  3:115042/131‑1 (MQ=255)                                                                                                                                            cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          >  4:115042/2‑132 (MQ=255)                                                                                                                                                 gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGttt    <  3:90506/149‑1 (MQ=255)                                                                                                                                                   tGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGa  >  7:207288/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGA  >  NC_002947/5390435‑5390729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑