Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,585	C→G	6.7%	intergenic (+674/‑48)	PP_4738 → / → PP_mr56	hypothetical protein/C4 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,585	0	C	G	6.7%	190.5 / 3.6	60	intergenic (+674/‑48)	PP_4738/PP_mr56	hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (23/32);  new base G (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGA  >  NC_002947/5390437‑5390729                                                                                                                                                     |                                                                                                                                                  gTCTTTTTTTGTGCCTGGAATTTGGTCGCTGCGGGTCTAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtc                                                                                                                                                   <  6:183172/149‑1 (MQ=255)    tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                 >  8:145989/1‑148 (MQ=255)    tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                 >  2:71503/1‑148 (MQ=255)        tttGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAAc                                                                                                                                             <  2:296192/148‑1 (MQ=255)            tgCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGCTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAccc                                                                                                                                        <  7:321026/149‑2 (MQ=255)              ccTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                      <  7:145989/149‑1 (MQ=255)              ccTGGAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                      >  5:235650/1‑149 (MQ=255)                tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCtt                                                                                                                                    >  7:280311/1‑149 (MQ=255)                  gAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                   <  6:235650/148‑1 (MQ=255)                   aaTTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGa                                                                                                                                 <  3:203802/149‑1 (MQ=255)                        gggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                            >  2:235395/1‑149 (MQ=255)                         ggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                            <  2:6925/148‑1 (MQ=255)                                gggggCGAAAAATCGGTTTTAACCCCTTTCTTGTAGGGCGTTTCCCTAAGTTACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCTGTGGGTCGGTGGTCATGAACGACCGGCTTTTACAGGCCGCAACGa                                                                                                                    <  8:6133/148‑1 (MQ=255)                                cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAAc                                                                                                                      <  2:27298/147‑1 (MQ=255)                                cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGa                                                                                                                    <  5:6000/149‑1 (MQ=255)                                           aTCGGATTTAACCCCTTTCTTGTAGGGCGTTTCGCAAAGATACTGCCTGCTGCTGTTTTCCGTTGCGGTCATTTTTTCATGTTTTTAGTCTCTNTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgcgcg                                                                                                        <  5:3443/149‑1 (MQ=255)                                            tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                           <  7:133309/145‑1 (MQ=255)                                            tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                           >  8:133309/1‑145 (MQ=255)                                                    aaCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTCTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGt                                                                                               >  2:342119/1‑149 (MQ=255)                                                        ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  4:212333/1‑149 (MQ=255)                                                        ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  1:287026/1‑149 (MQ=255)                                                        ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTAATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                           >  1:171038/1‑149 (MQ=255)                                                          ttacTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCTTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATgg                                                                                         >  5:278879/4‑149 (MQ=255)                                                             cTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGGTTTAGTCTCGGCGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCg                                                                                       <  6:20918/148‑1 (MQ=255)                                                                 tAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgt                                                                                  <  1:235395/149‑1 (MQ=255)                                                                  ggcgCGTTTCCCAACGATACTGCCTGCGCCTGTTTTCCGGTGCGGTCATTTTTTCAGGTTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtg                                                                                   <  8:288430/144‑1 (MQ=255)                                                                  aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgtg                                                                                 >  4:148229/1‑149 (MQ=255)                                                                  aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgta                                                                                 >  7:288430/1‑148 (MQ=255)                                                                    ggCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCa                                                                               <  1:71503/149‑1 (MQ=255)                                                                       gTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATgg                                                                            <  8:280311/149‑1 (MQ=255)                                                                            ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  4:90506/1‑149 (MQ=255)                                                                            ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  7:253383/1‑149 (MQ=255)                                                                            ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                       >  5:36425/1‑149 (MQ=255)                                                                              aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                 <  5:38505/121‑1 (MQ=255)                                                                              aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                 >  6:38505/1‑121 (MQ=255)                                                                              aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGc                                                                     >  5:105206/1‑149 (MQ=255)                                                                                      tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                   >  4:110183/1‑143 (MQ=255)                                                                                      tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                   <  3:110183/143‑1 (MQ=255)                                                                                         ctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGcc                                                          <  2:287026/149‑1 (MQ=255)                                                                                            cgcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGt                                                       <  2:219814/149‑1 (MQ=255)                                                                                              ccTGTTTTCCGGTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGGCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGttt                                                     <  4:131297/149‑1 (MQ=255)                                                                                                 gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   >  8:284225/1‑148 (MQ=255)                                                                                                 gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  7:284225/148‑1 (MQ=255)                                                                                                  ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgt                                                 <  6:36425/149‑1 (MQ=255)                                                                                                  ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  8:253383/147‑1 (MQ=255)                                                                                                  ttgtCCGCTGCGGTCATTTATTCAGTGGTTTAGTCTCTGTGCGTCGTTGCTCATCAACGACCGGCTTTGACAGCCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                   <  6:278879/144‑1 (MQ=255)                                                                                                       cGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCg                                            >  7:158511/1‑149 (MQ=255)                                                                                                         ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                         <  3:37400/134‑1 (MQ=255)                                                                                                         ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                         >  4:37400/1‑134 (MQ=255)                                                                                                           gCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                         <  3:148229/148‑1 (MQ=255)                                                                                                            cGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGt                                       <  3:212333/149‑1 (MQ=255)                                                                                                               tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTg                                    >  2:217380/1‑149 (MQ=255)                                                                                                                 aTTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGAACGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                   <  8:158511/148‑1 (MQ=255)                                                                                                                   tttttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                                >  8:110295/1‑149 (MQ=255)                                                                                                                         aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑AACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGGCAACCCATg                          >  1:282321/1‑149 (MQ=255)                                                                                                                         aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          <  6:105206/149‑1 (MQ=255)                                                                                                                                       ggTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca               <  1:342119/146‑1 (MQ=255)                                                                                                                                         tGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCTCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAtt          <  2:171038/149‑1 (MQ=255)                                                                                                                                         ccggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGAGTACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAt           >  1:74985/3‑149 (MQ=255)                                                                                                                                          cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          <  3:115042/131‑1 (MQ=255)                                                                                                                                          cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                          >  4:115042/2‑132 (MQ=255)                                                                                                                                               gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGttt    <  3:90506/149‑1 (MQ=255)                                                                                                                                                 tGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGa  >  7:207288/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGA  >  NC_002947/5390437‑5390729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑