Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,587	T→G	6.3%	intergenic (+676/‑46)	PP_4738 → / → PP_mr56	hypothetical protein/C4 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,587	0	T	G	6.3%	185.9 / 2.1	60	intergenic (+676/‑46)	PP_4738/PP_mr56	hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (24/32);  new base G (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCG  >  NC_002947/5390440‑5390734                                                                                                                                                    |                                                                                                                                                     tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                      >  8:145989/1‑148 (MQ=255) tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcat                                                                                                                                                      >  2:71503/1‑148 (MQ=255)     tttGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAAc                                                                                                                                                  <  2:296192/148‑1 (MQ=255)         tgCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGCTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAccc                                                                                                                                             <  7:321026/149‑2 (MQ=255)           ccTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                           <  7:145989/149‑1 (MQ=255)           ccTGGAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGc                                                                                                                                           >  5:235650/1‑149 (MQ=255)             tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCtt                                                                                                                                         >  7:280311/1‑149 (MQ=255)               gAATTAGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                        <  6:235650/148‑1 (MQ=255)                aaTTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGa                                                                                                                                      <  3:203802/149‑1 (MQ=255)                     gggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                                 >  2:235395/1‑149 (MQ=255)                      ggCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGc                                                                                                                                 <  2:6925/148‑1 (MQ=255)                             gggggCGAAAAATCGGTTTTAACCCCTTTCTTGTAGGGCGTTTCCCTAAGTTACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCTGTGGGTCGGTGGTCATGAACGACCGGCTTTTACAGGCCGCAACGa                                                                                                                         <  8:6133/148‑1 (MQ=255)                             cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAAc                                                                                                                           <  2:27298/147‑1 (MQ=255)                             cGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGa                                                                                                                         <  5:6000/149‑1 (MQ=255)                                        aTCGGATTTAACCCCTTTCTTGTAGGGCGTTTCGCAAAGATACTGCCTGCTGCTGTTTTCCGTTGCGGTCATTTTTTCATGTTTTTAGTCTCTNTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgcgcg                                                                                                             <  5:3443/149‑1 (MQ=255)                                         tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                                <  7:133309/145‑1 (MQ=255)                                         tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAgc                                                                                                                >  8:133309/1‑145 (MQ=255)                                                 aaCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTCTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGt                                                                                                    >  2:342119/1‑149 (MQ=255)                                                     ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                                >  4:212333/1‑149 (MQ=255)                                                     ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                                >  1:287026/1‑149 (MQ=255)                                                     ccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTAATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCAt                                                                                                >  1:171038/1‑149 (MQ=255)                                                       ttacTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCTTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATgg                                                                                              >  5:278879/4‑149 (MQ=255)                                                          cTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGGTTTAGTCTCGGCGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCg                                                                                            <  6:20918/148‑1 (MQ=255)                                                              tAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgt                                                                                       <  1:235395/149‑1 (MQ=255)                                                               ggcgCGTTTCCCAACGATACTGCCTGCGCCTGTTTTCCGGTGCGGTCATTTTTTCAGGTTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtg                                                                                        <  8:288430/144‑1 (MQ=255)                                                               aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgtg                                                                                      >  4:148229/1‑149 (MQ=255)                                                               aGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTtgta                                                                                      >  7:288430/1‑148 (MQ=255)                                                                 ggCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCa                                                                                    <  1:71503/149‑1 (MQ=255)                                                                    gTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATgg                                                                                 <  8:280311/149‑1 (MQ=255)                                                                         ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                            >  4:90506/1‑149 (MQ=255)                                                                         ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                            >  7:253383/1‑149 (MQ=255)                                                                         ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCAc                                                                            >  5:36425/1‑149 (MQ=255)                                                                           aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                      >  6:38505/1‑121 (MQ=255)                                                                           aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAAcc                                                                                                      <  5:38505/121‑1 (MQ=255)                                                                           aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGc                                                                          >  5:105206/1‑149 (MQ=255)                                                                                   tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                        >  4:110183/1‑143 (MQ=255)                                                                                   tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCtt                                                                        <  3:110183/143‑1 (MQ=255)                                                                                      ctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGcc                                                               <  2:287026/149‑1 (MQ=255)                                                                                         cgcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGt                                                            <  2:219814/149‑1 (MQ=255)                                                                                           ccTGTTTTCCGGTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGGCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGttt                                                          <  4:131297/149‑1 (MQ=255)                                                                                              gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                        <  7:284225/148‑1 (MQ=255)                                                                                              gTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                        >  8:284225/1‑148 (MQ=255)                                                                                               ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgt                                                      <  6:36425/149‑1 (MQ=255)                                                                                               ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                        <  8:253383/147‑1 (MQ=255)                                                                                               ttgtCCGCTGCGGTCATTTATTCAGTGGTTTAGTCTCTGTGCGTCGTTGCTCATCAACGACCGGCTTTGACAGCCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                        <  6:278879/144‑1 (MQ=255)                                                                                                    cGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCg                                                 >  7:158511/1‑149 (MQ=255)                                                                                                      ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                              <  3:37400/134‑1 (MQ=255)                                                                                                      ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                              >  4:37400/1‑134 (MQ=255)                                                                                                        gCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                              <  3:148229/148‑1 (MQ=255)                                                                                                         cGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGt                                            <  3:212333/149‑1 (MQ=255)                                                                                                            tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTg                                         >  2:217380/1‑149 (MQ=255)                                                                                                              aTTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGAACGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                        <  8:158511/148‑1 (MQ=255)                                                                                                                tttttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                                     >  8:110295/1‑149 (MQ=255)                                                                                                                      aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑AACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGGCAACCCATg                               >  1:282321/1‑149 (MQ=255)                                                                                                                      aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCACAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                               <  6:105206/149‑1 (MQ=255)                                                                                                                                    ggTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca                    <  1:342119/146‑1 (MQ=255)                                                                                                                                      tGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCTCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAtt               <  2:171038/149‑1 (MQ=255)                                                                                                                                      ccggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGAGTACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAt                >  1:74985/3‑149 (MQ=255)                                                                                                                                       cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                               <  3:115042/131‑1 (MQ=255)                                                                                                                                       cggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                               >  4:115042/2‑132 (MQ=255)                                                                                                                                            gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGttt         <  3:90506/149‑1 (MQ=255)                                                                                                                                              tGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGa       >  7:207288/1‑149 (MQ=255)                                                                                                                                                   atcaACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCg  >  5:110112/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGA‑TACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCG  >  NC_002947/5390440‑5390734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑