Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 374,771 C→T 8.3% P21S (CCG→TCG)  PP_0312 → electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947374,7710CT 8.3% 134.6 / 2.6 48P21S (CCG→TCG) PP_0312electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCACCGG  >  NC_002947/374626‑374919
                                                                                                                                                  |                                                                                                                                                     
tggtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGc                                                                                                                                                    >  7:83535/1‑149 (MQ=255)
tggtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCT‑AACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGACGAGTGGATCGCCCGTAACCGTCTGCACCCGCt                                                                                                                                                   >  7:340206/1‑149 (MQ=255)
  gtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTAATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCg                                                                                                                                                     <  1:268469/147‑1 (MQ=255)
   tggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGCGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGc                                                                                                                                                 <  3:188760/149‑1 (MQ=255)
     gCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCAt                                                                                                                                               <  8:231333/149‑1 (MQ=255)
        gACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGcc                                                                                                                                            <  4:49855/149‑1 (MQ=255)
             gCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GAt                                                                                                                                      >  1:21154/1‑149 (MQ=255)
                 aTCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATg                                                                                                                                     >  7:180866/1‑146 (MQ=255)
                     aggaggACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACgg                                                                                                                              <  4:228590/149‑2 (MQ=255)
                             gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCcgc                                                                                                                                          <  1:251144/130‑1 (MQ=255)
                               aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAg                                                                                                                    >  6:141254/1‑149 (MQ=255)
                                tCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGc                                                                                                                   >  7:91333/1‑149 (MQ=255)
                                  cACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCt                                                                                                                  <  8:91333/148‑1 (MQ=255)
                                   aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTg                                                                                                                 >  3:88321/1‑148 (MQ=255)
                                   aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACggttac                                                                                                                          <  3:42194/139‑6 (MQ=37)
                                   aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACggttac                                                                                                                          >  4:42194/1‑134 (MQ=37)
                                     tGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGAt                                                                                                              <  8:180866/149‑1 (MQ=255)
                                      gTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATg                                                                                                             >  4:8843/1‑149 (MQ=255)
                                       tCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgg                                                                                                            <  2:21154/149‑1 (MQ=255)
                                          cAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGAGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGc                                                                                                         >  4:278205/1‑149 (MQ=255)
                                           aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                                                          >  4:100751/1‑147 (MQ=255)
                                           aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                                                          <  3:100751/147‑1 (MQ=255)
                                                  cAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGc                                                                                                 <  6:69797/149‑1 (MQ=255)
                                                        gCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGAT‑CACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATcc                                                                                          >  4:190459/1‑149 (MQ=255)
                                                          tAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGCCCAGCTGGATGGGGCCCAACGGCATCATCCg                                                                                         >  6:93197/1‑149 (MQ=255)
                                                            aaCGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGc                                                                                        <  4:88321/148‑1 (MQ=255)
                                                                      tGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCcaca                                                                             >  6:359992/1‑149 (MQ=255)
                                                                        cGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGAT‑CACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                          <  3:190459/149‑1 (MQ=255)
                                                                          gAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCg                                                                         <  5:141254/149‑1 (MQ=255)
                                                                                            aCATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCCCATGCGATTGCCGCAGGCTTTGTc                                                       >  8:261968/1‑149 (MQ=255)
                                                                                                t‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcc                                                   >  4:124393/1‑149 (MQ=255)
                                                                                                   tCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCCTCATCCGCAAGACCCCACATGCGATTGCCGCAGGCTTTGTcggcccg                                                 >  4:38081/1‑149 (MQ=255)
                                                                                                     cgccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggc                                               >  8:306955/1‑149 (MQ=255)
                                                                                                       ccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccg                                             <  4:350297/149‑1 (MQ=255)
                                                                                                               cACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAg                                     <  8:340206/149‑1 (MQ=255)
                                                                                                                aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                    <  4:176553/149‑1 (MQ=255)
                                                                                                                aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                    >  3:176553/1‑149 (MQ=255)
                                                                                                                aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                    <  3:278205/149‑1 (MQ=255)
                                                                                                                          tGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCg                          >  1:183931/1‑149 (MQ=255)
                                                                                                                            gATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCa                        <  8:83535/149‑1 (MQ=255)
                                                                                                                                  ccGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAgcggcg                  >  4:18581/1‑149 (MQ=255)
                                                                                                                                         cGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCcag             >  7:193614/1‑147 (MQ=255)
                                                                                                                                              gCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGgcgc                 >  3:32948/1‑138 (MQ=255)
                                                                                                                                              gCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGgcgc                 <  4:32948/138‑1 (MQ=255)
                                                                                                                                               cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                          >  6:108847/1‑80 (MQ=255)
                                                                                                                                               cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                          <  5:108847/80‑1 (MQ=255)
                                                                                                                                               cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCAGCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCAc     <  3:38081/149‑1 (MQ=255)
                                                                                                                                                aCCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCAcc    >  3:286049/1‑149 (MQ=255)
                                                                                                                                                  ccGCTGCATGCCGC‑GATGAAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCAGCAAGAACCCACATGCGATTGCCCCAGGCTTTGTCGGCCCGGCCGGCATCAAGCCCATCGAACGCAGCGGCCCCCAGCACGGCACCgg  >  7:140028/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATT‑ATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCACCGG  >  NC_002947/374626‑374919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑