Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,477,000 G→A 79.1% W410* (TGG→TGA)  PP_4813 → PAP2 family protein/DedA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,477,0000GA79.1% 78.3 / 18.1 43W410* (TGG→TGA) PP_4813PAP2 family protein/DedA family protein
Reads supporting (aligned to +/- strand):  ref base G (3/6);  new base A (16/18);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

ATTGCCCTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTAAACCCGCTCCTACGCACGGGATCGCGC  >  NC_002947/5476857‑5477143
                                                                                                                                               |                                                                                                                                                
aTTGCCCTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCt                                                                                                                                             <  5:52120/149‑1 (MQ=255)
 ttGCCCTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTg                                                                                                                                            <  5:304565/149‑1 (MQ=255)
 ttGCCCTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTg                                                                                                                                            <  4:350347/149‑1 (MQ=255)
    cccTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGgtg                                                                                                                                         <  5:282282/149‑1 (MQ=255)
      cTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGtt                                                                                                                                       <  1:37590/149‑1 (MQ=255)
           gcgGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCgg                                                                                                                                 <  2:146685/149‑1 (MQ=255)
                 gtACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCg                                                                                                                           >  8:51946/1‑149 (MQ=255)
                    ccTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTgg                                                                                                                        >  8:163928/1‑149 (MQ=255)
                                cTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTGTCCGGCATGCGTGGCGCTGCTGGCg                                                                                                             <  7:51946/149‑1 (MQ=255)
                                   gCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGttct                                                                                                         <  1:151010/149‑1 (MQ=255)
                                     cAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGttcttc                                                                                                       <  3:178129/149‑1 (MQ=255)
                                     cAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGttcttc                                                                                                       >  3:235170/1‑149 (MQ=255)
                                      aaCCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCg                                                                                                      >  5:194903/1‑149 (MQ=255)
                                             ctCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCcatgc                                                                                                <  8:230952/147‑1 (MQ=255)
                                               ccTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGgcg                                                                                                                      >  3:58989/1‑124 (MQ=255)
                                               ccTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGgcg                                                                                                                      <  4:58989/124‑1 (MQ=255)
                                                      ggggCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGcc                                                                                      <  3:230610/149‑1 (MQ=255)
                                                             tgctgGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGAGTTGCAGCAGGCg                                                                               >  4:225770/1‑149 (MQ=255)
                                                                tgGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTg                                                                            >  1:67542/1‑149 (MQ=255)
                                                                    cTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGtggtgg                                                                                                                                               >  6:34742/1‑79 (MQ=255)
                                                                    cTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGtggtgg                                                                                                                                               <  5:34742/79‑1 (MQ=255)
                                                                    cTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCAGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGt                                                                          >  7:56528/1‑147 (MQ=255)
                                                                      gTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTg                                                                                                                                            >  4:226957/1‑80 (MQ=255)
                                                                      gTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTg                                                                                                                                            <  3:226957/80‑1 (MQ=255)
                                                                      gTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGcc                                                                                                    >  7:2965/1‑119 (MQ=255)
                                                                      gTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGcc                                                                                                    <  8:2965/119‑1 (MQ=255)
                                                                            tgtgCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCaa                                                                >  7:289910/1‑149 (MQ=255)
                                                                                cgcACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGc                                                                                                              <  4:281991/99‑1 (MQ=255)
                                                                                cgcACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGc                                                                                                              >  3:281991/1‑99 (MQ=255)
                                                                                       aGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTa                                                                    >  5:74749/1‑134 (MQ=255)
                                                                                       aGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTa                                                                    <  6:74749/134‑1 (MQ=255)
                                                                                       aGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTg                                                     >  7:188006/1‑149 (MQ=255)
                                                                                               aTTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGc                                              <  7:163928/148‑1 (MQ=255)
                                                                                               aTTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGATGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCtgtg                                               >  8:330146/1‑147 (MQ=255)
                                                                                                  ggTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGc                                          >  6:279834/1‑149 (MQ=255)
                                                                                                   gTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGc                                          <  8:188006/148‑1 (MQ=255)
                                                                                                        aCGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTc                                    <  6:194903/149‑1 (MQ=255)
                                                                                                           ccAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTcgcg                                 >  6:92665/1‑149 (MQ=255)
                                                                                                                        ccAGCCCTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTAAACCCGCTc                    <  5:279834/149‑1 (MQ=255)
                                                                                                                              cTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTaaa                           <  3:191423/136‑1 (MQ=255)
                                                                                                                              cTGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTaaa                           >  4:191423/1‑136 (MQ=255)
                                                                                                                               tGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCgg                                >  1:70802/1‑130 (MQ=255)
                                                                                                                               tGCCGCTGCGGGTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCgg                                <  2:70802/130‑1 (MQ=255)
                                                                                                                                         ggTGTGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTAAACCCGCTCCTACGCACGGGATcgcg   <  8:289910/149‑1 (MQ=255)
                                                                                                                                          gtgtGATGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGGAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTAAACCCGCTCCTACGCACGGGATCgcgc  <  7:330146/149‑1 (MQ=255)
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ATTGCCCTGTCGCGGGTGTACCTGGGGGCGCACTGGCCAACCGATATCCTGGCCGGGGCCCTGCTGGCCTGTTGCGTGTGCGCACTCAGCCTGACATTGGTGCAACGCCAGCAGACCCTGCCAGCCCTGCCGCTGCGGGTGTGGTGGCTGGTGTTG‑CCGGCATGCGTGGCGCTGCTGGCGTTCTTCGCCATGCATGCGTTGCCGCAGGCGTTGGTCAGGTATCAATATTGATGTTGCCTGTGCCGGCCTATTCGCGGGTAAACCCGCTCCTACGCACGGGATCGCGC  >  NC_002947/5476857‑5477143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑