Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,503,135	C→A	8.8%	L29M (CTG→ATG) ‡	oprC →	copper receptor OprC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,503,135	0	C	A	8.8%	212.2 / 8.5	68	L29M (CTG→ATG) ‡	oprC	copper receptor OprC
Reads supporting (aligned to +/- strand):  ref base C (29/33);  new base A (2/4);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

CAACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCG  >  NC_002947/5502987‑5503282                                                                                                                                                      |                                                                                                                                                     caacaTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGccc                                                                                                                                                      >  2:53001/1‑149 (MQ=255)  aacaTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCt                                                                                                                                                     >  8:295526/1‑149 (MQ=255)   acaTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTg                                                                                                                                                    >  6:34826/1‑149 (MQ=255)    caTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTgg                                                                                                                                                   <  5:152468/149‑1 (MQ=255)             cACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGcc                                                                                                                                         <  3:158191/149‑1 (MQ=255)              aCGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCCCCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGccc                                                                                                                                        >  8:153526/1‑149 (MQ=255)                  aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGc                                                                                                                                    <  3:225896/149‑1 (MQ=255)                   gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGcc                                                                                                                                   <  8:327853/149‑1 (MQ=255)                   gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCAGGGCCGG‑CGGccagggc                                                                                                                                    >  7:57997/1‑143 (MQ=255)                      aggaggCTTCGCGCTAACTCATGCCTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCAGGGCCGG‑CGGCCAGGGCCATg                                                                                                                                <  4:207927/149‑1 (MQ=255)                      aggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTTTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATg                                                                                                                                <  1:263030/149‑1 (MQ=255)                      aggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATg                                                                                                                                <  8:175472/149‑1 (MQ=255)                       ggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGa                                                                                                                               <  1:125995/149‑1 (MQ=255)                       ggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCAGGGCCGG‑CGGCCAGGGCCATgg                                                                                                                               <  8:57997/149‑2 (MQ=255)                        gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGaa                                                                                                                              <  5:34826/149‑1 (MQ=255)                             ttCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCa                                                                                                                         >  1:218107/1‑149 (MQ=255)                               cgcgCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCTCAT‑GGCCAGGGCCGG‑CGGCCAGGGCCATgggcgcga                                                                                                                         <  6:169438/147‑8 (MQ=38)                                 cgcTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCAtgt                                                                                                                      <  1:53001/149‑1 (MQ=255)                                 cgcTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCAtgtg                                                                                                                     >  8:91360/1‑149 (MQ=255)                                  gcTAACTCATGACTGATCGACTGGAATCATTATGTACGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCAtgtg                                                                                                                     >  6:369153/1‑148 (MQ=255)                                  gcTAACTCATGACTGATCGACT‑GAATAATTATGTACCGGGGCAACCCGGTTTTTGCCATTTGCCATCGTGGGAACATACCCGCCCATTTCCGCAAGCATCACGCCCCCAA‑GGCCCTTGCCGC‑CGGCCCAGGCCAATAAAACCAAGTGCa                                                                                                                   >  6:3021/1‑149 (MQ=255)                                   cTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCa                                                                                                                   <  7:153526/149‑1 (MQ=255)                                       cTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCacgac                                                                                                               <  7:295526/149‑1 (MQ=255)                                        tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCAGGGCCGG‑CGGCCAGGGCCATg                                                                                                                                <  6:320308/131‑1 (MQ=255)                                        tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCAGGGCCGG‑CGGCCAGGGCCATg                                                                                                                                >  5:320308/1‑131 (MQ=255)                                             aCTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCAcc                                                                                                          <  6:71793/148‑1 (MQ=255)                                                    gACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGa                                                                                                  <  5:369153/149‑1 (MQ=255)                                                    gACTGGAAACATTATGTCCCCCTGAACCCCTGTTTTTCACTAGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGA‑CGGCCCAGGCCAAGAACACCATGTACACGAAGCACCCCAGATGa                                                                                                  >  2:34493/1‑149 (MQ=255)                                                     aCTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAg                                                                                                 <  7:207879/149‑1 (MQ=255)                                                      cTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                >  1:233507/1‑149 (MQ=255)                                                      cTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                <  7:91360/149‑1 (MQ=255)                                                         gAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGccc                                                                                              >  6:205154/1‑148 (MQ=255)                                                            tCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGccgc‑cg                                                                                                                                            <  6:104720/99‑1 (MQ=255)                                                            tCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCACTGGccgc‑cg                                                                                                                                            >  5:104720/1‑99 (MQ=255)                                                               ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCacga                                                                                                                <  6:257590/124‑1 (MQ=255)                                                               ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCacga                                                                                                                >  5:257590/1‑124 (MQ=255)                                                                 aTGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCTTGCGTGGGACCATCGCCGCACTGTGCGGCTCGCTGCTCGCGCCCTT‑GGCCCTGGCCGC‑CGTCCCTGTCCATGAAGTGCATGTTCACGAGTGACGCGTGTTGAGCCCGACGGTGa                                                                                      <  1:34493/148‑1 (MQ=255)                                                                   gTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCa                                                                                   >  5:102817/1‑149 (MQ=255)                                                                            cACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTgg                                                                          >  8:241047/1‑149 (MQ=255)                                                                                       ttGCCTTGTCCCTGCGGGGGACCATCGCGGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCGGGCCATGAAGCCCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTc                                                                <  8:284938/148‑1 (MQ=255)                                                                                         gCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                              <  6:102817/148‑1 (MQ=255)                                                                                           cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGCCGCACCCGAGTTGAGCCCGTCGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                           >  4:273580/1‑149 (MQ=255)                                                                                           cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGt                                                                                                     <  7:233353/107‑1 (MQ=255)                                                                                           cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGt                                                                                                     >  8:233353/1‑107 (MQ=255)                                                                                                cccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACt                                                      >  2:284234/1‑149 (MQ=255)                                                                                                 ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGtt                                                                                                    >  1:279898/1‑102 (MQ=255)                                                                                                 ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGtt                                                                                                    <  2:279898/102‑1 (MQ=255)                                                                                                 ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtg                                                     >  8:166321/1‑149 (MQ=255)                                                                                                 ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtg                                                     >  1:235820/1‑149 (MQ=255)                                                                                                     cGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                   <  2:233507/147‑1 (MQ=255)                                                                                                      gTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTc                                                <  5:3021/149‑1 (MQ=255)                                                                                                         ggACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCAc                                              <  7:241047/148‑1 (MQ=255)                                                                                                           aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCaa                                           >  8:275647/1‑149 (MQ=255)                                                                                                             cATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                         <  7:166321/149‑1 (MQ=255)                                                                                                              aTCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGCCGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAccc                                        >  3:188767/1‑149 (MQ=255)                                                                                                                  ccgccCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaaa                                    <  2:235820/149‑1 (MQ=255)                                                                                                                  ccgccCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaaa                                    <  5:69678/149‑1 (MQ=255)                                                                                                                     cccTGTGCGGCTGGCTGCTCGCGGCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGACGACCTTGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCTCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAc                                 <  4:191176/149‑1 (MQ=255)                                                                                                                       cTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAccc                               <  7:275647/149‑1 (MQ=255)                                                                                                                        tgtgCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcg                              >  5:359251/1‑149 (MQ=255)                                                                                                                            cGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGCCGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgcc                          >  5:196396/1‑149 (MQ=255)                                                                                                                               cTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                       >  5:55534/1‑149 (MQ=255)                                                                                                                                tCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                       >  7:209051/1‑148 (MQ=255)                                                                                                                                   ctgctCGCGCCCAT‑GGCCCTGGCCGCGCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGccagccag                    >  1:185625/1‑149 (MQ=255)                                                                                                                                       tCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                   <  2:328469/145‑1 (MQ=255)                                                                                                                                       tCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                   >  1:328469/1‑145 (MQ=255)                                                                                                                                         gcgcCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc             <  7:338154/149‑1 (MQ=255)                                                                                                                                         gcgcCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc             <  6:196396/149‑1 (MQ=255)                                                                                                                                         gcgcCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc             >  3:94732/1‑149 (MQ=255)                                                                                                                                                    cccTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGgc   <  6:55534/149‑1 (MQ=255)                                                                                                                                                     ccTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGgcg  <  4:94732/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CAACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAT‑GGCCCTGGCCGC‑CGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCG  >  NC_002947/5502987‑5503282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑