Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,532,759 A→G 6.1% V142A (GTC→GCC)  braD ← high‑affinity branched‑chain amino acid ABC transporter permease BraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,532,7590AG 6.1% 188.8 / 2.3 65V142A (GTC→GCC) braDhigh‑affinity branched‑chain amino acid ABC transporter permease BraD
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base G (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTA  >  NC_002947/5532613‑5532907
                                                                                                                                                  |                                                                                                                                                    
aGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACt                                                                                                                                                    >  2:10654/1‑149 (MQ=255)
 ggCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACtt                                                                                                                                                   <  7:311230/149‑1 (MQ=255)
            ggTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATg                                                                                                                                        <  7:64360/149‑1 (MQ=255)
             gTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATgg                                                                                                                                       >  5:33565/1‑149 (MQ=255)
                atGCTGGGTAGCACGGCACATGAGGCCGAGATTGCTGTACTTGATCACCTAGGTCAGCAGGACCATGCCGAGAAAGGCCGCCACCAGGATCAAGATCATGCTATAAGTCTGTTGAACGAAGCCGCACCCAACGTGGCCACCCATGgcgc                                                                                                                                    >  8:335641/2‑149 (MQ=255)
                  gCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCTGACCCGACTTCGACACGCATGGCGcc                                                                                                                                   <  6:294046/148‑1 (MQ=255)
                  gCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGcc                                                                                                                                   <  6:125913/148‑1 (MQ=255)
                       gTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGTCGGACCCGACTTCGACACGCATGGCGCCTTc                                                                                                                                >  6:293781/1‑146 (MQ=255)
                            aCGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAggg                                                                                                                        >  1:288731/1‑149 (MQ=255)
                              ggCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCcagca                                                                                                                           >  7:44010/1‑144 (MQ=255)
                              ggCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCcagca                                                                                                                           <  8:44010/144‑1 (MQ=255)
                              ggCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGtt                                                                                                                      <  8:206531/149‑1 (MQ=255)
                              ggCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGtt                                                                                                                      >  5:65488/1‑149 (MQ=255)
                               gCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTg                                                                                                                     <  2:99447/149‑1 (MQ=255)
                                cacaTGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTgg                                                                                                                    >  6:26462/1‑149 (MQ=255)
                                   aTGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCAc                                                                                                                 >  6:113253/1‑149 (MQ=255)
                                                 ggTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGGCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggcgg                                                                                                    <  5:85065/148‑1 (MQ=255)
                                                     tACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGcc                                                                                               <  6:49021/149‑1 (MQ=255)
                                                          gATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGc                                                                                          >  7:209966/1‑149 (MQ=255)
                                                           aTCACGTAGGTCAGGAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGc                                                                                          <  7:335641/148‑1 (MQ=255)
                                                           aTCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGc                                                                                          <  6:181938/148‑1 (MQ=255)
                                                              aCGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGAt                                                                                      <  4:326553/149‑1 (MQ=255)
                                                                   ggTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                                  <  5:26462/148‑1 (MQ=255)
                                                                    gTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGc                                                                                >  2:77989/1‑149 (MQ=255)
                                                                       agcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                                  >  6:332032/1‑144 (MQ=255)
                                                                       agcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                                  <  5:332032/144‑1 (MQ=255)
                                                                       agcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCg                                                                               >  1:11587/1‑147 (MQ=255)
                                                                        gcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTc                                                                                                                                <  2:261409/97‑1 (MQ=255)
                                                                        gcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTc                                                                                                                                >  1:261409/1‑97 (MQ=255)
                                                                         cagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGt                                                                           >  6:328291/1‑149 (MQ=255)
                                                                                   ccGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAg                                                                    >  7:148488/1‑146 (MQ=255)
                                                                                   ccGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAg                                                                    <  8:148488/146‑1 (MQ=255)
                                                                                     gACAAAGGCCGCCACCAGGATGAAAATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGAt                                                                 >  7:223376/1‑147 (MQ=255)
                                                                                      aCAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGc                                                                                                          <  8:81565/105‑1 (MQ=255)
                                                                                      aCAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGc                                                                                                          >  7:81565/1‑105 (MQ=255)
                                                                                        aaaGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                             >  5:197017/1‑148 (MQ=255)
                                                                                          aGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGa                                                           >  6:248375/1‑148 (MQ=255)
                                                                                          aGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCCGTGCGTAGTTCTGCAGGATCAGCGaa                                                          <  1:10654/149‑1 (MQ=255)
                                                                                              cgccACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGc                                                      <  1:77989/149‑1 (MQ=255)
                                                                                                  accaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGAt                                                  <  8:209966/149‑1 (MQ=255)
                                                                                                  accaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGAt                                                  <  6:33565/149‑1 (MQ=255)
                                                                                                   ccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGTCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATg                                                 >  3:52200/1‑149 (MQ=255)
                                                                                                   ccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGAt                                                                                      <  6:207412/112‑1 (MQ=255)
                                                                                                   ccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGAt                                                                                      >  5:207412/1‑112 (MQ=255)
                                                                                                      ggATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCa                                              <  6:197017/149‑1 (MQ=255)
                                                                                                           aaGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGAt                                         <  6:65488/149‑1 (MQ=255)
                                                                                                                   ggTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGTCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGc                                     <  4:52200/145‑1 (MQ=255)
                                                                                                                    gTATAAGTCAGTTGCACGAAGTCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTg                                 <  5:293781/148‑1 (MQ=255)
                                                                                                                     tataAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGa                                          >  6:165961/1‑138 (MQ=255)
                                                                                                                     tataAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGa                                          <  5:165961/138‑1 (MQ=255)
                                                                                                                     tataAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGc                                >  1:246439/1‑148 (MQ=255)
                                                                                                                           tCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCgg                         <  2:11587/149‑1 (MQ=255)
                                                                                                                              gTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTg                      >  5:269060/1‑149 (MQ=255)
                                                                                                                                  cACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATgg                                                <  1:160567/119‑1 (MQ=255)
                                                                                                                                  cACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATgg                                                >  2:160567/1‑119 (MQ=255)
                                                                                                                                  cACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAg                   <  5:113253/148‑1 (MQ=255)
                                                                                                                                  cACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGCGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt                  >  6:7759/1‑149 (MQ=255)
                                                                                                                                   tcGAAGTCGGGTCCGGCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGt                       >  8:143677/2‑143 (MQ=255)
                                                                                                                                   tcGAAGTCGGGTCCGGCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGt                       <  7:143677/142‑1 (MQ=255)
                                                                                                                                   tcGAAGTCGGGTCCGGCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt                  >  7:299609/2‑148 (MQ=255)
                                                                                                                                     gAAGTCGGGTCCGGCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTAc               <  8:299609/149‑1 (MQ=255)
                                                                                                                                               ccGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCggg                        <  8:283258/130‑1 (MQ=255)
                                                                                                                                                 gACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACg              <  6:152940/138‑1 (MQ=255)
                                                                                                                                                 gACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACg              >  5:152940/1‑138 (MQ=255)
                                                                                                                                                 gACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGAGGGCGCCCTCGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGTTGGAGTTACGAAGAGGTATg    >  3:282792/1‑148 (MQ=255)
                                                                                                                                                  aCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTg    >  4:159912/1‑147 (MQ=255)
                                                                                                                                                  aCTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTa  <  2:246439/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATGGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTA  >  NC_002947/5532613‑5532907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑