Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 374,782 C→A 9.3% A24A (GCC→GCA)  PP_0312 → electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947374,7820CA 9.3% 123.7 / 4.1 43A24A (GCC→GCA) PP_0312electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (22/17);  new base A (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

GACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCACCGGCGCGGGCGGGC  >  NC_002947/374634‑374930
                                                                                                                                                    |                                                                                                                                                     
gACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGcc                                                                                                                                                       <  4:49855/149‑1 (MQ=255)
     gCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GAt                                                                                                                                                 >  1:21154/1‑149 (MQ=255)
         aTCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATg                                                                                                                                                >  7:180866/1‑146 (MQ=255)
             aggaggACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACgg                                                                                                                                         <  4:228590/149‑2 (MQ=255)
                     gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCcgc                                                                                                                                                     <  1:251144/130‑1 (MQ=255)
                       aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAg                                                                                                                               >  6:141254/1‑149 (MQ=255)
                        tCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGc                                                                                                                              >  7:91333/1‑149 (MQ=255)
                          cACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCt                                                                                                                             <  8:91333/148‑1 (MQ=255)
                           aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTg                                                                                                                            >  3:88321/1‑148 (MQ=255)
                           aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACggttac                                                                                                                                     <  3:42194/139‑6 (MQ=37)
                           aCTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACggttac                                                                                                                                     >  4:42194/1‑134 (MQ=37)
                             tGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGAt                                                                                                                         <  8:180866/149‑1 (MQ=255)
                              gTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATg                                                                                                                        >  4:8843/1‑149 (MQ=255)
                               tCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgg                                                                                                                       <  2:21154/149‑1 (MQ=255)
                                  cAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGAGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGc                                                                                                                    >  4:278205/1‑149 (MQ=255)
                                   aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                                                                     <  3:100751/147‑1 (MQ=255)
                                   aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                                                                     >  4:100751/1‑147 (MQ=255)
                                          cAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGc                                                                                                            <  6:69797/149‑1 (MQ=255)
                                                gCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGAT‑CACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATcc                                                                                                     >  4:190459/1‑149 (MQ=255)
                                                  tAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGCCCAGCTGGATGGGGCCCAACGGCATCATCCg                                                                                                    >  6:93197/1‑149 (MQ=255)
                                                    aaCGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGc                                                                                                   <  4:88321/148‑1 (MQ=255)
                                                              tGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCcaca                                                                                        >  6:359992/1‑149 (MQ=255)
                                                                cGGAGGTGCACTGATGAGCGACATTATCCGCCGCGAT‑CACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                                     <  3:190459/149‑1 (MQ=255)
                                                                  gAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCg                                                                                    <  5:141254/149‑1 (MQ=255)
                                                                                    aCATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCCCATGCGATTGCCGCAGGCTTTGTc                                                                  >  8:261968/1‑149 (MQ=255)
                                                                                        tATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcc                                                              >  4:124393/1‑149 (MQ=255)
                                                                                          tCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCCTCATCCGCAAGACCCCACATGCGATTGCCGCAGGCTTTGTcggcccg                                                            >  4:38081/1‑149 (MQ=255)
                                                                                            cgccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggc                                                          >  8:306955/1‑149 (MQ=255)
                                                                                              ccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccg                                                        <  4:350297/149‑1 (MQ=255)
                                                                                                      cACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAg                                                <  8:340206/149‑1 (MQ=255)
                                                                                                       aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                               <  4:176553/149‑1 (MQ=255)
                                                                                                       aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                               >  3:176553/1‑149 (MQ=255)
                                                                                                       aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc                                               <  3:278205/149‑1 (MQ=255)
                                                                                                                 tGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCg                                     >  1:183931/1‑149 (MQ=255)
                                                                                                                   gATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCa                                   <  8:83535/149‑1 (MQ=255)
                                                                                                                         ccGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAgcggcg                             >  4:18581/1‑149 (MQ=255)
                                                                                                                                cGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCcag                        >  7:193614/1‑147 (MQ=255)
                                                                                                                                     gCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGgcgc                            <  4:32948/138‑1 (MQ=255)
                                                                                                                                     gCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGgcgc                            >  3:32948/1‑138 (MQ=255)
                                                                                                                                      cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                                     >  6:108847/1‑80 (MQ=255)
                                                                                                                                      cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                                                                     <  5:108847/80‑1 (MQ=255)
                                                                                                                                      cACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCAGCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCAc                <  3:38081/149‑1 (MQ=255)
                                                                                                                                       aCCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCAcc               >  3:286049/1‑149 (MQ=255)
                                                                                                                                         ccGCTGCATGCCGC‑GATGAAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCAGCAAGAACCCACATGCGATTGCCCCAGGCTTTGTCGGCCCGGCCGGCATCAAGCCCATCGAACGCAGCGGCCCCCAGCACGGCACCgg             >  7:140028/1‑149 (MQ=255)
                                                                                                                                           gCTGCATGCCGC‑GATGCAGACGCAACAGACCAGATGGATGGGGCCCATCGGCATCATCCGCAAGAACCCTCATGCGTTTGCCGCAGGCTTTGTCGGCCCGGGCGGCATCAAGCGCATCGACCGCAGAGGCGCCCAGCAGGGCACCTgcg           <  8:140028/149‑1 (MQ=255)
                                                                                                                                                    cgc‑gATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCACCGGCgcgggcgggc  <  2:183931/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATCGACCGCAGCGGCGCCCAGCAGGGCACCGGCGCGGGCGGGC  >  NC_002947/374634‑374930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑