Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,623,395 G→T 9.4% R115R (CGG→AGG)  PP_4941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,623,3950GT 9.4% 155.7 / 5.7 53R115R (CGG→AGG) PP_4941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base T (3/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCA  >  NC_002947/5623255‑5623543
                                                                                                                                            |                                                                                                                                                    
ccGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTc                                                                                                                                                >  7:289432/1‑147 (MQ=255)
  ggCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCTCGGGTCgagc                                                                                                                                            <  3:76005/149‑2 (MQ=255)
    cattGCCGGCTGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  3:69455/146‑1 (MQ=255)
    cAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCgaga                                                                                                                                            >  7:191325/1‑147 (MQ=255)
     aaTGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAg                                                                                                                                         >  3:259046/1‑149 (MQ=255)
       tGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCACTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCCCGCCATCGACGCCACGCTCGAACGCTTGCAGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAAGGTCGAGAAAgcg                                                                                                                                       >  7:331041/1‑149 (MQ=255)
              cgcgCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAAc                                                                                                                                <  5:145169/149‑1 (MQ=255)
                     tCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          >  1:126016/1‑132 (MQ=255)
                     tCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGaaa                                                                                                                                          <  2:126016/132‑1 (MQ=255)
                                  gCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAg                                                                                                            >  2:173756/1‑149 (MQ=255)
                                    cTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGtt                                                                                                          <  3:300030/149‑1 (MQ=255)
                                       cAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCaa                                                                                                       <  4:34491/149‑1 (MQ=255)
                                         gTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGc                                                                                                     <  1:173756/149‑1 (MQ=255)
                                          tGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAgc                                                                                                                            <  8:154851/125‑1 (MQ=255)
                                          tGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAgc                                                                                                                            >  7:154851/1‑125 (MQ=255)
                                          tGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCa                                                                                                    >  3:145807/1‑149 (MQ=255)
                                            gcgcAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCTCGGGTCgagctg                                                                                                                                          >  3:10462/1‑106 (MQ=255)
                                            gcgcAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCTCGGGTCgagctg                                                                                                                                          <  4:10462/109‑4 (MQ=255)
                                               cagcaAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAgg                                                                                               <  7:270115/149‑1 (MQ=255)
                                               cagcaAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAgg                                                                                               <  4:259046/149‑1 (MQ=255)
                                                  caAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGtt                                                                                            >  7:254459/1‑149 (MQ=255)
                                                   aaGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGttt                                                                                           >  1:279766/1‑149 (MQ=255)
                                                    aGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTtc                                                                                          >  6:103062/1‑149 (MQ=255)
                                                        ccGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTtcgtcg                                                                                      >  3:221231/1‑149 (MQ=255)
                                                         cGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGc                                                                                                                 <  6:262889/121‑1 (MQ=255)
                                                         cGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGc                                                                                                                 >  5:262889/1‑121 (MQ=255)
                                                          gTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGcc                                                                                    <  2:322384/149‑1 (MQ=255)
                                                             aGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCtt                                                                                  <  5:103062/148‑1 (MQ=255)
                                                                  aaGGCAGCGCACGCCAGCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTGTCGTCGCCTTGCAAc                                                                             <  8:289432/148‑1 (MQ=255)
                                                                  aaGGATGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTAGCCAGACAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCACAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCTCCATCCAACg                                                                            >  7:270108/1‑149 (MQ=255)
                                                                    ggAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTg                                                                          >  7:202696/1‑149 (MQ=255)
                                                                          gcACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAAt                                                                      >  3:142050/1‑147 (MQ=255)
                                                                             cGCCATCGACGCCCCGCGCGCACGATTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGCCCCGAAGGTCGAGAAAGCTCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGTGTTTCGTCGCCTTGCAACGTGCAATCAGcc                                                                 <  8:331041/149‑1 (MQ=255)
                                                                                cATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTg                                                              <  7:157333/149‑1 (MQ=255)
                                                                                    gACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGagcc                                                          <  8:202696/149‑1 (MQ=255)
                                                                                        ccACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGa                                                             >  6:320685/1‑142 (MQ=255)
                                                                                        ccACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGa                                                             <  5:320685/142‑1 (MQ=255)
                                                                                                           gATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGATGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCAGCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGa                                   <  8:270108/149‑1 (MQ=255)
                                                                                                               ggCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACct                               <  2:119352/149‑1 (MQ=255)
                                                                                                                gCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctg                              >  1:90977/1‑149 (MQ=255)
                                                                                                                gCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctg                              >  3:279885/1‑149 (MQ=255)
                                                                                                                   tGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctgctg                           <  8:254459/149‑1 (MQ=255)
                                                                                                                   tGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctgctg                           >  6:76594/1‑149 (MQ=255)
                                                                                                                     ttCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACctgctg                           <  3:14237/147‑1 (MQ=255)
                                                                                                                         ccagccagCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGACCCTGCTGCACCt                      >  6:234396/1‑148 (MQ=255)
                                                                                                                              cagcagCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCGGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGc                >  6:355857/1‑149 (MQ=255)
                                                                                                                                 tttcTCGACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCGATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCg              >  5:310021/4‑148 (MQ=255)
                                                                                                                                  agcTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGaa            <  4:145807/149‑1 (MQ=255)
                                                                                                                                  agcTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGaa            <  2:90977/149‑1 (MQ=255)
                                                                                                                                     tCGACCCTCGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTc         >  5:86194/1‑149 (MQ=255)
                                                                                                                                     tCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCAc                        >  6:282984/1‑134 (MQ=255)
                                                                                                                                       gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGaa            >  7:195751/1‑144 (MQ=255)
                                                                                                                                       gACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGaa            <  8:195751/144‑1 (MQ=255)
                                                                                                                                            gAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCa  <  4:142050/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CCGGCAATGCCGGCCGCGCAGTCGCAACGTACCGGCCTGCAGTGGCGCAGCAAGACCCGTCAGGTTAAGGAAGCGCACGCCATCGACGCCACGCTCGAACGCTTGCCGATCGGCCTGTTCGCCAGCCAGCAGCTCGACCCGAGGGTCGAGAAAGCGCCCCAACCAGCTGCTTTTTAGCCGCAGTTCAAGCACCAGGGTTTCGTCGCCTTGCAACGTGCAATCAGCCTTGAGCCAGCCATCGTGCAGGCTGGCAGGAACCTGCTGCACCTGATCGCCGAAGTCGTAGGCA  >  NC_002947/5623255‑5623543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑