Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,646,266 A→T 6.8% D540V (GAC→GTC)  PP_4954 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,646,2660AT 6.8% 217.2 / 5.3 73D540V (GAC→GTC) PP_4954membrane protein
Reads supporting (aligned to +/- strand):  ref base A (32/36);  new base T (2/3);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

CCACTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCCACAACGCTGAC  >  NC_002947/5646128‑5646414
                                                                                                                                          |                                                                                                                                                    
ccACTGCCGCGATCACCCTGTTGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGc                                                                                                                                            >  3:329449/1‑149 (MQ=255)
 cACTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGcc                                                                                                                                           <  1:146054/149‑1 (MQ=255)
 cACTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCAGTcaggcaggaac                                                                                                                                           <  6:174859/149‑4 (MQ=255)
  aCTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGcc                                                                                                                                           >  3:219369/1‑148 (MQ=255)
   cTGCCGCGATCCCCCTGAGGGGGCTGTTCTGCTTCACCCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGa                                                                                                                                         <  2:108637/149‑1 (MQ=255)
   cTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCAGTcaggcaggaacag                                                                                                                                         <  7:300751/149‑6 (MQ=255)
      ccGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACgg                                                                                                                                      <  4:164657/149‑1 (MQ=255)
      ccGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACgg                                                                                                                                      <  4:159148/149‑1 (MQ=255)
      ccGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACgg                                                                                                                                      >  1:39345/1‑149 (MQ=255)
      ccGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACgg                                                                                                                                      >  3:73605/1‑149 (MQ=255)
              aCCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAAc                                                                                                                                >  2:326232/1‑147 (MQ=255)
                ccTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAgg                                                                                                                            >  7:243247/1‑149 (MQ=255)
                     tGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTg                                                                                                                       <  4:73605/149‑1 (MQ=255)
                       gggcggTTCTGCTTCAACCAGCTCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTgg                                                                                                                      <  6:251201/143‑1 (MQ=255)
                       gTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGc                                                                                                                     <  1:77244/149‑1 (MQ=255)
                         gCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCt                                                                                                                                    <  1:140940/132‑1 (MQ=255)
                         gCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCt                                                                                                                                    >  2:140940/1‑132 (MQ=255)
                          cTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCTCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCaa                                                                                                                  <  4:329449/149‑1 (MQ=255)
                          cTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCaa                                                                                                                  >  4:316699/1‑149 (MQ=255)
                           tGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCaa                                                                                                                  >  4:341138/1‑148 (MQ=255)
                            gTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGc                                                                                                                                            <  3:166401/121‑1 (MQ=255)
                            gTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGc                                                                                                                                            >  4:166401/1‑121 (MQ=255)
                              tCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTCCCTGCCAGTcaggcaggaacagg                                                                                                                                        <  6:281401/123‑7 (MQ=255)
                              tCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTCCCTGCCAGTcaggcaggaacagg                                                                                                                                        >  5:281401/1‑117 (MQ=255)
                                  cTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTg                                                                                                           <  3:341138/148‑1 (MQ=255)
                                   ttCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATAccc                                                                                                             <  8:267995/145‑1 (MQ=255)
                                   ttCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATAccc                                                                                                             >  7:267995/1‑145 (MQ=255)
                                      aaCCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCt                                                                                                       <  4:127517/148‑1 (MQ=255)
                                          aGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTgcgc                                                                                                   >  6:4733/1‑148 (MQ=255)
                                                 cGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTGTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCgtgt                                                                                           <  8:243247/149‑1 (MQ=255)
                                                   aTGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTAc                                                                                         <  5:4733/149‑1 (MQ=255)
                                                       cTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTgc                                                                                     <  2:39345/149‑1 (MQ=255)
                                                           ggCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTgcgc                                                                                   >  5:136685/1‑147 (MQ=255)
                                                             ccTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTgcgc                                                                                   >  7:288420/1‑145 (MQ=255)
                                                             ccTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAgt                                                                               <  6:136685/149‑2 (MQ=255)
                                                                   ccTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATg                                                                          <  4:219369/148‑1 (MQ=255)
                                                                       ccGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgcagca                                                                     <  4:81726/149‑1 (MQ=255)
                                                                       ccGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgcag                                                                       >  8:144444/1‑147 (MQ=255)
                                                                         gcgcCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGcagcag                                                                    >  8:248288/1‑148 (MQ=255)
                                                                         gcgcCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGcagcag                                                                    >  1:22778/1‑148 (MQ=255)
                                                                           gcCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTAt                                                                 <  1:326232/149‑1 (MQ=255)
                                                                                 tCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgca                                                                        >  3:237848/1‑136 (MQ=255)
                                                                                 tCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgca                                                                        <  4:237848/136‑1 (MQ=255)
                                                                                    aTACCCTGGTCGGCAGCCTGATTGCCATTCTTCCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACgg                                                         >  6:286661/1‑148 (MQ=255)
                                                                                        ccTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGc                                                                                               <  3:31150/106‑1 (MQ=255)
                                                                                        ccTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGc                                                                                               >  4:31150/1‑106 (MQ=255)
                                                                                        caggcgcGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGTCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAgc                                                    <  3:308376/143‑1 (MQ=255)
                                                                                            gTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGcagcag                                                                    >  5:69837/1‑129 (MQ=255)
                                                                                            gTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGcagcag                                                                    <  6:69837/129‑1 (MQ=255)
                                                                                            gTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGcgcg                                                 <  2:22778/148‑1 (MQ=255)
                                                                                            gTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGcga                                                >  1:197308/1‑149 (MQ=255)
                                                                                                    ccTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTgg                                        <  5:286661/149‑1 (MQ=255)
                                                                                                          tGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTAcc                                  >  5:286737/1‑149 (MQ=255)
                                                                                                           gCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTAcc                                  <  7:248288/148‑1 (MQ=255)
                                                                                                            ccATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGc                                >  6:217992/1‑149 (MQ=255)
                                                                                                             cATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGcc                               >  1:333259/1‑149 (MQ=255)
                                                                                                              aTTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCt                              >  4:279632/1‑149 (MQ=255)
                                                                                                                  ttGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGGTGAACAAGGCGATGGCCAATACCCTGGACTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGcc                          >  1:140991/1‑149 (MQ=255)
                                                                                                                    gCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCg                        >  4:88341/1‑149 (MQ=255)
                                                                                                                      aGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgc                                                                         >  7:257507/1‑98 (MQ=255)
                                                                                                                      aGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATgc                                                                         <  8:257507/98‑1 (MQ=255)
                                                                                                                      aGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGgc                      <  3:316699/149‑1 (MQ=255)
                                                                                                                          ttcctgttcctgCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTg                                         >  4:293314/1‑126 (MQ=255)
                                                                                                                          ttcctgttcctgCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTg                                         <  3:293314/126‑1 (MQ=255)
                                                                                                                            cctgttcctgCCTGACTGGCAGGGCCGACGGCTGAACATGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTTTGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAAt                 <  8:288420/148‑1 (MQ=255)
                                                                                                                            cctgttcctgCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAAt                 <  3:279632/148‑1 (MQ=255)
                                                                                                                            cctgttcctgCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATg                >  1:170011/1‑149 (MQ=255)
                                                                                                                                gccctgccagTCAGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCca            >  5:39612/3‑149 (MQ=255)
                                                                                                                                  cctgcctgACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCcac           <  5:217992/148‑1 (MQ=255)
                                                                                                                                   ctgcctgACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCCACaa         <  3:88341/149‑1 (MQ=255)
                                                                                                                                    tgcctgACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGc                                       <  7:246032/118‑1 (MQ=255)
                                                                                                                                    tgcctgACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGc                                       >  8:246032/1‑118 (MQ=255)
                                                                                                                                      cctgACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCCACAACGc      <  6:286737/149‑1 (MQ=255)
                                                                                                                                          aCTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCCACAACGCTGAc  >  8:113267/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
CCACTGCCGCGATCACCCTGATGGTGCTGTTCTGCTTCAACCAGATCGGCGATGGCTACGGCCTGTTCCTGCCGCGCCTGTTCGATACCCTGGTCGGCAGCCTGATTGCCATTCTTGCAGTGTTCCTGTTCCTGCCTGACTGGCAGGGCCGACGGCTGAACAAGGCGCTGGCCAATACCCTGGCCTGCGCCAGCGTGTACCTGCGCCAGATCATGCAGCAGTATGCCCACGGCAAGCGCGACGACCTGGCCTACCGCCTGGCCCGGCGCAATGCCCACAACGCTGAC  >  NC_002947/5646128‑5646414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑