Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,143 A→C 7.3% I257L (ATC→CTC)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1430AC 7.3% 163.2 / 4.8 55I257L (ATC→CTC) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base A (28/23);  new base C (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTG  >  NC_002947/5679002‑5679280
                                                                                                                                             |                                                                                                                                         
gAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGt                                                                                                                                    >  5:165253/1‑149 (MQ=255)
     ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGGCGATCTCGTCGTggata                                                                                                                               <  4:143678/149‑4 (MQ=255)
                tCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCTGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGAAGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGACGAAcc                                                                                                                    >  5:199504/1‑149 (MQ=255)
                    cgcCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATc                                                                                                                                         >  7:94033/1‑124 (MQ=255)
                     gcCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCAc                                                                                                               >  3:175862/1‑149 (MQ=255)
                     gcCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCAc                                                                                                               <  2:228974/149‑1 (MQ=255)
                      ccGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCAc                                                                                                               >  6:160406/1‑148 (MQ=255)
                             cgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGGGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCg                                                                                                       >  4:183187/1‑149 (MQ=255)
                                      gCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAg                                                                                               >  4:350276/1‑148 (MQ=255)
                                              aGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCgg                                                                                       >  1:2152/1‑148 (MQ=255)
                                                           cATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAtt                                                                         >  5:363960/1‑149 (MQ=255)
                                                            aTGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAttt                                                                        >  8:175438/1‑149 (MQ=255)
                                                               cgcATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTcc                                                                      <  6:165253/148‑1 (MQ=255)
                                                               cgcATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCt                                                                     <  6:81494/149‑1 (MQ=255)
                                                                  aTGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgtg                                                                  <  2:2152/149‑1 (MQ=255)
                                                                   tGTACCACACGGTGTAACTCAAGTTTTTGAGCGAGGCCTGAGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGc                                                                 <  6:199504/149‑1 (MQ=255)
                                                                    gTACCACCCGGTGTACCTCAAGTTGTTGGGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGcc                                                                >  1:204033/1‑149 (MQ=255)
                                                                       ccaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTg                                                              <  5:160406/148‑1 (MQ=255)
                                                                             ggTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAgg                                                       <  2:204033/149‑1 (MQ=255)
                                                                               tgtACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                     >  2:50329/1‑149 (MQ=255)
                                                                                   ccTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTc                                                                                                        >  8:251609/1‑94 (MQ=255)
                                                                                   ccTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTc                                                                                                        <  7:251609/94‑1 (MQ=255)
                                                                                   ccTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGa                                                 >  4:82109/1‑149 (MQ=255)
                                                                                     tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                               >  8:102546/1‑149 (MQ=255)
                                                                                      cAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTc                                                            <  6:336828/135‑1 (MQ=255)
                                                                                      cAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTc                                                            >  5:336828/1‑135 (MQ=255)
                                                                                          ttgttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcggcgg                                          <  8:26951/149‑1 (MQ=255)
                                                                                             ttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTcc                                                           >  7:241456/1‑129 (MQ=255)
                                                                                             ttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTcc                                                           <  8:241456/129‑1 (MQ=255)
                                                                                             ttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTa                                       >  8:135633/1‑149 (MQ=255)
                                                                                               gcgcGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTAc                                      <  3:110848/148‑1 (MQ=255)
                                                                                                      gCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCt                              >  5:87725/1‑149 (MQ=255)
                                                                                                       ccTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTg                             <  1:50329/149‑1 (MQ=255)
                                                                                                        cTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTgg                            <  3:32478/149‑1 (MQ=255)
                                                                                                           cGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCATCCGCACGATGTTAGCCATCGAGCAAGCCGGCATACCCCCGGAATTCCTGTGCCTGTACAAGGCCCATACCGGCGGCTACCTGCCGCTGgccg                         >  1:277262/1‑149 (MQ=255)
                                                                                                                gCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                    <  4:175862/149‑1 (MQ=255)
                                                                                                                gCTATGACGTGCACCTTATCCACGACGAGATCGCCGGGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                    <  3:183187/149‑1 (MQ=255)
                                                                                                                 cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                     <  7:44771/115‑1 (MQ=255)
                                                                                                                 cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                     >  8:44771/1‑115 (MQ=255)
                                                                                                                 cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTAc                                      >  1:65833/1‑130 (MQ=255)
                                                                                                                 cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTAc                                      <  2:65833/130‑1 (MQ=255)
                                                                                                                  tATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCaa                                                         >  4:276586/1‑110 (MQ=255)
                                                                                                                  tATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCaa                                                         <  3:276586/110‑1 (MQ=255)
                                                                                                                        gTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGc                                                                                <  8:31839/81‑1 (MQ=255)
                                                                                                                        gTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGc                                                                                >  7:31839/1‑81 (MQ=255)
                                                                                                                        gTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCAGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac            >  4:34388/1‑149 (MQ=255)
                                                                                                                           cACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac            <  4:129410/146‑1 (MQ=255)
                                                                                                                           cACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac            >  3:129410/1‑146 (MQ=255)
                                                                                                                           cACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGa         >  4:36414/1‑149 (MQ=255)
                                                                                                                           cACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGa         >  4:229315/1‑149 (MQ=255)
                                                                                                                           cACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGa         <  6:87725/149‑1 (MQ=255)
                                                                                                                                 aTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg     <  3:82109/147‑1 (MQ=255)
                                                                                                                                  cccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg  >  3:344610/2‑149 (MQ=255)
                                                                                                                                    cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg     >  5:76884/1‑144 (MQ=255)
                                                                                                                                    cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg     <  6:76884/144‑1 (MQ=255)
                                                                                                                                            gaTCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac            >  7:243587/1‑129 (MQ=255)
                                                                                                                                            gaTCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac            <  8:243587/129‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                         
GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTG  >  NC_002947/5679002‑5679280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑