Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 385,476 C→G 10.0% intergenic (+16/+19) PP_0320 → / ← ltaE methyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947385,4760CG10.0% 134.9 / 2.8 46intergenic (+16/+19)PP_0320/ltaEmethyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base C (26/15);  new base G (3/2);  total (29/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCC  >  NC_002947/385332‑385621
                                                                                                                                                |                                                                                                                                                 
aCGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggcg                                                                                                                                               <  6:155224/149‑1 (MQ=255)
       aGGGGATTTCGGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCgg                                                                                                                                        <  4:221863/149‑1 (MQ=255)
        ggggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTTCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGAGGCAGATGGGTGGGTTCAGGCTCTCGATCGCAGGTGCCGCTCGGCGGCCCCGGc                                                                                                                                       >  8:145777/1‑149 (MQ=255)
         tggATTTCCGATTTGGCGGGTGAGACGGCTGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTTGCGTATTACCTGGCGCTGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAGGGGGGCCCCGGct                                                                                                                                      <  8:93510/148‑1 (MQ=255)
            aTTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCtctc                                                                                                                                   <  6:180618/149‑1 (MQ=255)
              ttCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGc                                                                                                                                 >  4:305619/1‑149 (MQ=255)
                ccGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCt                                                                                                                                >  8:233700/1‑148 (MQ=255)
                                  cggcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGttc                                                                                                             >  7:120296/1‑147 (MQ=255)
                                   ggcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCg                                                                                                            <  3:305619/149‑1 (MQ=255)
                                          gAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATcc                                                                                                     >  6:50309/1‑149 (MQ=255)
                                             gTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccgcc                                                                                                  <  6:98105/149‑1 (MQ=255)
                                               gCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCcgccgc                                                                                                >  1:163020/1‑149 (MQ=255)
                                                cAAGGGTGCCGCGAGGAGTGCCAGGCGTGGCGGGGGTTGGCTGAGGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCgccgcc                                                                                               <  7:262819/149‑1 (MQ=255)
                                                         cgcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACg                                                                                       >  1:245239/1‑148 (MQ=255)
                                                          gcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAg                                                                                             >  1:144797/1‑141 (MQ=255)
                                                           cgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAc                                                                                    >  3:147080/1‑149 (MQ=255)
                                                           cgAGGAGTGCCAGGCGTTGCGGGGGTGGGCGCATGACCTGGCGCGGCAGATGGGTCGGGTCAGGCGCTAGATCGCAGGGCCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAc                                                                                    <  1:83079/149‑1 (MQ=255)
                                                             aggagTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCCGCGCGCGCGCAGCCCCCAATGATCGTGCGGATATACGCCGCCAGCTCACGCAccc                                                                                  >  2:355851/1‑149 (MQ=255)
                                                                 gTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCgcgc                                                                              <  2:245239/149‑1 (MQ=255)
                                                                    ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAg                                                                                             >  6:89104/1‑131 (MQ=255)
                                                                    ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAg                                                                                             <  5:89104/131‑1 (MQ=255)
                                                                      aGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGcttctt                                                                         >  3:174129/1‑149 (MQ=255)
                                                                             gCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATc                                                                  >  5:13886/1‑149 (MQ=255)
                                                                                   gTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                        <  5:71994/137‑1 (MQ=255)
                                                                                   gTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                        >  6:71994/1‑137 (MQ=255)
                                                                                       gCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGccacca                                                                                                                       >  1:207118/1‑86 (MQ=255)
                                                                                         tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCTGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACAt                                                      <  7:233700/149‑1 (MQ=255)
                                                                                          gatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCACACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACAt                                                      >  1:211813/1‑148 (MQ=255)
                                                                                                 tGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcg                                              >  5:313696/1‑149 (MQ=255)
                                                                                                 tGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCACGGCTCTCGCACAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcg                                              >  5:256864/1‑149 (MQ=255)
                                                                                                         cAGATGGGTGGGTTCAGGCTTTTTATCGCTGGGGCCGCAGGGGGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGc                                                                                            <  4:194391/95‑1 (MQ=255)
                                                                                                         cAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGc                                                                                            >  3:194391/1‑95 (MQ=255)
                                                                                                                   ggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATa                             >  7:135819/1‑148 (MQ=255)
                                                                                                                    gtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACGTGAAGCGCGCGCCACCGCTGCCGATaaa                           <  6:13886/149‑1 (MQ=255)
                                                                                                                       cAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAgg                         <  4:147080/148‑1 (MQ=255)
                                                                                                                           ctctAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                              <  4:190303/107‑1 (MQ=255)
                                                                                                                           ctctAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                              >  3:190303/1‑107 (MQ=255)
                                                                                                                                    gCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                   >  7:147826/1‑109 (MQ=255)
                                                                                                                                    gCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                   <  8:147826/109‑1 (MQ=255)
                                                                                                                                     ccggggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGc          >  3:300810/3‑149 (MQ=255)
                                                                                                                                      cggggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGGTGCCGATAAAGGTATAGAAGCGCCAGcc         >  5:211419/2‑149 (MQ=255)
                                                                                                                                        gggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCt       >  6:158830/1‑149 (MQ=255)
                                                                                                                                          gCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCg                                >  2:344836/1‑122 (MQ=255)
                                                                                                                                          gCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCg                                <  1:344836/122‑1 (MQ=255)
                                                                                                                                           ccGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGc    >  4:298124/1‑149 (MQ=255)
                                                                                                                                             gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc  >  5:87340/1‑149 (MQ=255)
                                                                                                                                             gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc  >  8:243144/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCC  >  NC_002947/385332‑385621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑