Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,722,966 C→A 6.1% V85L (GTG→TTG)  PP_5023 ← amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,722,9660CA 6.1% 213.4 / 3.7 65V85L (GTG→TTG) PP_5023amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (38/23);  new base A (2/2);  total (40/25)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

CCCCGGACACCACCGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCACTC  >  NC_002947/5722819‑5723113
                                                                                                                                                   |                                                                                                                                                   
ccccGGACACCACCGAGATCCACAAGGTGGTCCACAGGCCCCATGACAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGCTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGATGCAGGCTGTCCTTGTACACATCGAGCAca                                                                                                                                                    >  4:96991/1‑148 (MQ=255)
     gACACCACCGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTc                                                                                                                                               <  5:266117/149‑1 (MQ=255)
     gACACCACCGAGATCCACAAGCTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGATACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTc                                                                                                                                               <  5:310841/149‑1 (MQ=255)
       caccacCGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCgg                                                                                                                                             <  7:142878/149‑1 (MQ=255)
          caccGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCAAGTGCTCgat                                                                                                                                            >  5:266222/1‑145 (MQ=255)
          caccGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCAAGTGCTCgat                                                                                                                                            <  6:266222/147‑3 (MQ=255)
           accGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGcac                                                                                                                                          >  4:26704/1‑148 (MQ=255)
            ccGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCAAGTGCTCgat                                                                                                                                            <  3:125950/145‑3 (MQ=255)
            ccGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCAAGTGCTCgat                                                                                                                                            >  4:125950/1‑143 (MQ=255)
             cGAGATCCAGAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGcaccac                                                                                                                                       <  3:96991/149‑1 (MQ=255)
                 aTCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTc                                                                                                                                   <  3:26704/149‑1 (MQ=255)
                  tCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTc                                                                                                                                   <  4:25560/148‑1 (MQ=255)
                    cacaAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTc                                                                                                                                <  2:101824/149‑1 (MQ=255)
                     acaAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTcc                                                                                                                               >  7:261800/1‑149 (MQ=255)
                     acaAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTcc                                                                                                                               >  2:129944/1‑149 (MQ=255)
                          gtggtCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTc                                                                                                                                   <  4:318309/140‑1 (MQ=255)
                          gtggtCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTc                                                                                                                                   >  3:318309/1‑140 (MQ=255)
                                    ggCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCgg                                                                                                                >  5:228783/1‑149 (MQ=255)
                                      ccccATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGc                                                                                                              <  1:129944/149‑1 (MQ=255)
                                        ccATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGt                                                                                                            >  2:85118/1‑149 (MQ=255)
                                        ccATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCGGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGt                                                                                                            >  7:101811/1‑149 (MQ=255)
                                         cATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGtt                                                                                                           >  3:86835/1‑149 (MQ=255)
                                               aGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCAcc                                                                                                     <  4:237659/149‑1 (MQ=255)
                                                   ggCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGaa                                                                                                 <  6:228783/149‑1 (MQ=255)
                                                      ccGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAAt                                                                                               >  3:209269/1‑148 (MQ=255)
                                                                  ggCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTg                                                                                   >  2:297452/1‑148 (MQ=255)
                                                                     gggTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGTAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGcaca                                                                               <  2:149481/149‑1 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACtt                                                                                                                                     >  7:177318/1‑88 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACtt                                                                                                                                     <  8:177318/88‑1 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGcaca                                                                               >  7:280917/1‑142 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGcaca                                                                               <  8:280917/142‑1 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCg                                                                         >  4:43746/1‑148 (MQ=255)
                                                                            cccGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCg                                                                         >  6:298940/1‑148 (MQ=255)
                                                                                     gTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAg                                                               >  7:322587/1‑149 (MQ=255)
                                                                                         ccTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGc                                                                           <  8:209003/133‑1 (MQ=255)
                                                                                         ccTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGc                                                                           >  7:209003/1‑133 (MQ=255)
                                                                                         ccTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGcgcg                                                           >  1:153003/1‑149 (MQ=255)
                                                                                           ttCGGCAACATCATCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGtt                                                         >  8:227479/1‑149 (MQ=255)
                                                                                               gCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTttgt                                                     >  8:85237/1‑149 (MQ=255)
                                                                                                 aaCATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGttt                                                   >  8:86776/1‑149 (MQ=255)
                                                                                                          gccgcGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGcc                                           <  2:153003/148‑1 (MQ=255)
                                                                                                                     gTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAg                               >  2:116057/1‑149 (MQ=255)
                                                                                                                      ttGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAg                               >  1:196113/1‑148 (MQ=255)
                                                                                                                      ttGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAg                               >  8:203929/1‑148 (MQ=255)
                                                                                                                          aGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCTGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTg                          <  8:261800/149‑1 (MQ=255)
                                                                                                                          aGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTg                          >  7:4325/1‑149 (MQ=255)
                                                                                                                                tCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAc                    >  8:249120/1‑149 (MQ=255)
                                                                                                                                  cTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAcgc                  >  1:314168/1‑149 (MQ=255)
                                                                                                                                  cTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAcgc                  >  7:209100/1‑149 (MQ=255)
                                                                                                                                   ttGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAcgcg                 >  1:27695/1‑149 (MQ=255)
                                                                                                                                       ccACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTc             >  5:116145/1‑149 (MQ=255)
                                                                                                                                        cacaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTc             >  2:231042/1‑148 (MQ=255)
                                                                                                                                         acaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGc                         <  8:81225/135‑1 (MQ=255)
                                                                                                                                         acaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGc                         >  7:81225/1‑135 (MQ=255)
                                                                                                                                         acaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAcg                   >  3:330751/1‑141 (MQ=255)
                                                                                                                                         acaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTAcg                   <  4:330751/141‑1 (MQ=255)
                                                                                                                                         acaTCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGACGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTc             >  6:184454/1‑147 (MQ=255)
                                                                                                                                          caTCGATCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAg          <  8:101811/149‑1 (MQ=255)
                                                                                                                                            tCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAgcg        <  5:143059/149‑1 (MQ=255)
                                                                                                                                              gAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAgcgc       >  3:41970/1‑148 (MQ=255)
                                                                                                                                              gAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAgcgc       >  6:172170/1‑148 (MQ=255)
                                                                                                                                               aGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACTCGGTTCCAGCGcc      >  2:256668/1‑148 (MQ=255)
                                                                                                                                               aGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCa     <  1:231042/149‑1 (MQ=255)
                                                                                                                                                 cACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCACt   >  8:195796/1‑149 (MQ=255)
                                                                                                                                                  aCTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCACTc  <  7:327921/149‑1 (MQ=255)
                                                                                                                                                  aCTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCACTc  >  8:327921/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCCCGGACACCACCGAGATCCACAAGGTGGTCCACAGGCCCCATGCCAGCGGGCCCGCGGCCCAATGGCGGGTTACCCCGATCTGGTCGCCTTCGGCAACATCGTCGCCGCGGCTCAGTTGCAGGCTGTCCTTGTCCACATCGAGCACTTGCTCGGCACCACTTTCGTCCTTCAGCGTGACGCGGGCGTTGTCACCGGAAATGACGATTTCCTGCACAGTGCCGTAGCCGGCAGCGCGTTGTGTTTCCTCGGCCTTGTAGGCGAAGTACTGCGGTACGCGGTTCCAGCGCCACTC  >  NC_002947/5722819‑5723113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑