Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,792,221 G→A 8.5% G370G (GGC→GGT)  gltD ← glutamate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,792,2210GA 8.5% 133.9 / 2.2 47G370G (GGC→GGT) gltDglutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base A (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CTTGGCTGTCCAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTGCGCGAACCCGGCATGTTGGCTT  >  NC_002947/5792073‑5792360
                                                                                                                                                      |                                                                                                                                           
cTTGGCTGTCCAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCg                                                                                                                                             >  8:233272/1‑149 (MQ=255)
   ggCTGTCCAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGc                                                                                                                                            <  3:222063/147‑1 (MQ=255)
        tCCAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAgg                                                                                                                                       <  7:195077/147‑1 (MQ=255)
          cAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGccc                                                                                                                                          <  8:38414/142‑1 (MQ=255)
          cAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGccc                                                                                                                                          >  7:38414/1‑142 (MQ=255)
                aaTGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCGCGTAGGCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGtctc                                                                                                                               <  1:103231/149‑1 (MQ=255)
                 aTGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGAc                                                                                                                            <  6:47845/149‑1 (MQ=255)
                     cATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACt                                                                                                                        >  5:323808/1‑149 (MQ=255)
                       tGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACttt                                                                                                                      >  4:119863/1‑149 (MQ=255)
                               aaaCCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCACCCAGGCGAGTCTCGACCACTTTGACGCCtt                                                                                                              >  7:92493/1‑149 (MQ=255)
                                aaCCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTc                                                                                                             >  1:44262/1‑149 (MQ=255)
                                 aCCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGTGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCg                                                                                                            >  3:302945/1‑149 (MQ=255)
                                    accgcgCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTCCCGGCAGGATCGCATCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGAcc                                                                                                         <  8:138146/144‑1 (MQ=255)
                                    aCGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCACGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGAcc                                                                                                         >  2:14922/1‑149 (MQ=255)
                                      ggTGCCGTGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCtt                                                                                                       <  3:91605/149‑1 (MQ=255)
                                        ttccgttcTCGGGCGGCAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCTACCtt                                                                                                       <  3:96379/140‑1 (MQ=255)
                                            gggCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGAcc                                                                                                         <  5:32622/141‑1 (MQ=255)
                                            gggCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGAcc                                                                                                         >  6:32622/1‑141 (MQ=255)
                                                        aaGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCAcaatgg                                                                                      >  1:194472/1‑148 (MQ=255)
                                                        aaGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCACGCCCAGGCGAGTCTCGACCACTTTGACGCTTTCGACCTTGTCCTCGCCCAcaatggc                                                                                     <  1:14922/149‑1 (MQ=255)
                                                             gAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCAcaatggcaatgg                                                                                <  4:160222/149‑1 (MQ=255)
                                                                ggCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCt                                                                             <  3:119863/149‑1 (MQ=255)
                                                                        ccACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGtt                                                                      <  6:323808/148‑1 (MQ=255)
                                                                        ccACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTg                                                                     <  7:233272/149‑1 (MQ=255)
                                                                        ccACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCACCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGtt                                                                      <  8:92493/148‑1 (MQ=255)
                                                                         cACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTg                                                                     >  7:260353/1‑148 (MQ=255)
                                                                            ggCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTaga                                                                 <  5:327786/149‑1 (MQ=255)
                                                                               gTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGa                                                              <  2:194472/149‑1 (MQ=255)
                                                                                    ccGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGTGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACtt                                                          <  4:302945/148‑1 (MQ=255)
                                                                                        cAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGc                                                     <  8:260353/149‑1 (MQ=255)
                                                                                         aGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGcc                                                    >  8:92235/1‑149 (MQ=255)
                                                                                         aGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGcc                                                    >  5:140149/1‑149 (MQ=255)
                                                                                           gATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCct                                                  >  2:125178/1‑149 (MQ=255)
                                                                                             tctcTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCctct                                                >  3:36656/1‑149 (MQ=255)
                                                                                                 ttCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCt                                            >  6:266178/1‑149 (MQ=255)
                                                                                                   cG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGCCGGTTGTAGCGGAACTTCACGCCCTCTTCCTTg                                          >  2:115247/1‑149 (MQ=255)
                                                                                                             gggATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCACAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCt                                   >  7:28686/1‑148 (MQ=255)
                                                                                                                aTTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAAc                               <  7:92235/149‑1 (MQ=255)
                                                                                                                     cTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCtt                          >  7:255517/1‑149 (MQ=255)
                                                                                                                      tCAGGGCTGCGGCGGCCACGGGCATCAGGCTCACCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCctct                                                <  7:198914/126‑1 (MQ=255)
                                                                                                                      tCAGGGCTGCGGCGGCCACGGGCATCAGGCTCACCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCctct                                                >  8:198914/1‑126 (MQ=255)
                                                                                                                        aGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTgc                       >  5:181437/1‑149 (MQ=255)
                                                                                                                          ggCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTgcgc                     >  5:314643/1‑149 (MQ=255)
                                                                                                                                         gggCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTGCGCGAACCCGGCATGTTg      <  5:266178/149‑1 (MQ=255)
                                                                                                                                            gtcgagacTCGCCTGGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAAAGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTACTTGGCGGTCTTAACCTCGTTGCGCGAACCCGGCATGTTGGCt   >  6:232638/8‑149 (MQ=255)
                                                                                                                                             aTCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCtt                          <  7:82462/125‑1 (MQ=255)
                                                                                                                                             aTCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCtt                          >  8:82462/1‑125 (MQ=255)
                                                                                                                                             aTCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTGCGCGAACCCGGCATGTTGGCtt  <  6:181437/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                           
CTTGGCTGTCCAGCTGAATGCCATGCTGTTCAAACCACGGTGCCGGGCTCGGGCGGAAGCCGAAGGCGATTACCACGGCGTCTGCCGGCAGGATCTCTTCG‑G‑AGCCCGGGATTGGCTCAGGGCTGCGGCGGCCACGGGCATCAGGCTCGCCCAGGCGAGTCTCGACCACTTTGACGCCTTCGACCTTGTCCTCGCCCACAATGGCAATGGGCTGACGGTTGTAGAGGAACTTCACGCCCTCTTCCTTGGCGTTCTTAACCTCTTTGCGCGAACCCGGCATGTTGGCTT  >  NC_002947/5792073‑5792360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑