Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,810,350 G→A 9.7% D81D (GAC→GAT) ‡ rpmE ← 50S ribosomal protein L31

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,810,3500GA 9.7% 111.6 / 2.1 42D81D (GAC→GAT) ‡rpmE50S ribosomal protein L31
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GCCCACAAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAACTACTTCGTAGTTCGGGTGGATA  >  NC_002947/5810213‑5810494
                                                                                                                                         |                                                                                                                                                
gCCCACAAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACttt                                                                                                                                       >  5:243465/1‑149 (MQ=255)
gCCCACAAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACttt                                                                                                                                       <  3:160245/149‑1 (MQ=255)
  ccACAAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCt                                                                                                                                     >  8:139323/1‑149 (MQ=255)
   cacaAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTg                                                                                                                                    >  2:226596/1‑149 (MQ=255)
    acaAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGc                                                                                                                                   >  7:14764/1‑149 (MQ=255)
    acaAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTAAGCAGAAAGTTCTg                                                                                                                                    >  1:182371/1‑148 (MQ=255)
          aaGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCg                                                                                                                             <  1:226596/149‑1 (MQ=255)
                 ccACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTAAGCAGAAAGTTCTGGACACCGgtggtcg                                                                                                                      <  2:182371/149‑5 (MQ=38)
                   acaAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAAc                                                                                                                    <  8:14764/149‑1 (MQ=255)
                    caAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACg                                                                                                                   <  6:188249/149‑1 (MQ=255)
                       tgggcGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACggg                                                                                                                 <  3:255406/144‑1 (MQ=255)
                         gACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTgg                                                                                                              <  6:48694/149‑1 (MQ=255)
                          aCGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTgg                                                                                                              >  8:71616/1‑148 (MQ=255)
                            gCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGca                                                                                                            >  5:326980/1‑148 (MQ=255)
                                   tcAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTg                                                                                                     >  7:296681/1‑148 (MQ=255)
                                    cAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGtt                                                                                                      <  6:243465/146‑1 (MQ=255)
                                       caacaaTGCGAAACCCGAAGGATTCGCCATGCGAATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAAc                                                                                                <  7:139323/149‑1 (MQ=255)
                                         acaaTGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTATTGGCACAGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACAACAGGTGTACAGCACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGAAAACg                                                                                               >  7:335060/1‑148 (MQ=255)
                                                       gAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACg                                                                                >  2:227794/1‑149 (MQ=255)
                                                                gCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCAAAGGTTGCAAACGtc                                                                                             >  3:53931/1‑127 (MQ=255)
                                                                      cgcATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGaa                                                                 <  6:326980/149‑1 (MQ=255)
                                                                         aTGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAAc                                                                >  5:24916/1‑147 (MQ=255)
                                                                           gATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACTCGTTGCACACGTTCAAAAATTATATCAAAAGGAAGTTGCCCAGGGTCGAACGAGt                                                            >  8:153857/1‑149 (MQ=255)
                                                                            aTTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGtt                                                           <  3:89575/149‑1 (MQ=255)
                                                                               aCTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTc                                                         <  8:296681/148‑1 (MQ=255)
                                                                                 ttcttGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGaa                                                      >  2:85428/1‑149 (MQ=255)
                                                                                      ggCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACtt                                                   <  7:71616/147‑1 (MQ=255)
                                                                                       gCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCa                                                                         >  2:287466/1‑124 (MQ=255)
                                                                                       gCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCa                                                                         <  1:287466/124‑1 (MQ=255)
                                                                                        cACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTa                                               <  1:227794/149‑1 (MQ=255)
                                                                                        cACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTa                                               >  2:46710/1‑149 (MQ=255)
                                                                                           cGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCg                                            >  5:300592/1‑149 (MQ=255)
                                                                                                    cGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTTTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAg                                   <  7:153857/149‑1 (MQ=255)
                                                                                                             cGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGc                           <  6:24916/148‑1 (MQ=255)
                                                                                                               gCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAac                        >  7:38240/1‑149 (MQ=255)
                                                                                                                        tGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGATCTAGTTGCACACGTTGAAAAAATCTATCTACAGGAAGTTGCCCAGGGTAGAACGAGATTCGAACTTGATACAGCAGCTGCAGGTGACTGCAACTACTTCTTa               >  4:69571/1‑149 (MQ=255)
                                                                                                                            cgcgACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAACTACTTCGTAGTTc           <  1:46710/149‑1 (MQ=255)
                                                                                                                             gcgACAACCGGTGTCAAGAACTTTCTGCTTACCGGTGTAGAACGGTTGGCACACGTTGAAAACATCTATCGACAGGACGTTCACCAGGGTAGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTTAATGAAACTACTTCGTAGtaag          >  8:230035/1‑146 (MQ=255)
                                                                                                                                accaccGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAACTACTTCGTAGTTCGGGt       <  3:97657/149‑1 (MQ=255)
                                                                                                                                 acaccGGTAAGCAGAAAGTTCTGGACACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAac                        >  1:132643/2‑131 (MQ=38)
                                                                                                                                 acaccGGTAAGCAGAAAGTTCTGGACACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAac                        <  2:132643/130‑1 (MQ=38)
                                                                                                                                     cGGTGTCCAGAACTTTCTGCTTACCGGTGTGGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAACTACTTCGTAGTTCGGGTGGATa  >  8:337271/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                
GCCCACAAAAAAGGCGCCCACAAGGGACGCCTTCTTCAGCAACAATGCGAAACCCGAAGGATTCGCCATGCGCATGATTACTTCTTGGCACCGAACATGCCGAAGCGATCGGCGAACTTCTGTACGCGACCACCGGTGTCCAGAACTTTCTGCTTACCGGTGTAGAACGGGTGGCACAGGTTGCAAACGTCGATCGACAGGACGTTGCCCAGGGTCGAACGAGTTTCGAACTTGTTACCGCAGCTGCAGGTGACTGCAACTACTTCGTAGTTCGGGTGGATA  >  NC_002947/5810213‑5810494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑