Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,849,936	C→G	6.1%	P214R (CCG→CGG)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,849,936	0	C	G	6.1%	205.7 / 3.7	65	P214R (CCG→CGG)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (30/31);  new base G (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

CATGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCAACTTCAAAGCGT  >  NC_002947/5849790‑5850082                                                                                                                                                   |                                                                                                                                                     cATGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCgg                                                                                                                                                    <  4:74523/149‑1 (MQ=255) cATGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCgg                                                                                                                                                    >  7:334147/1‑149 (MQ=255) cATGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCgg                                                                                                                                                    <  2:294303/149‑1 (MQ=255)  aTGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGCAGCCCTGGGCCTGGCCCTGCCggg                                                                                                                                                   >  4:100247/1‑149 (MQ=255)        aTCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCGGGGCAACgg                                                                                                                                             >  5:201713/1‑149 (MQ=255)        aTCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCGGGGCAACgg                                                                                                                                             >  5:206730/1‑149 (MQ=255)        aTCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACg                                                                                                                                              <  7:27223/148‑1 (MQ=255)         tCGCGGCCGACTCCACGGCCTCGGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCACCGGt                                                                                                                                            >  7:54651/1‑149 (MQ=255)         tCGCGGCCGACTCCACGGCCTCCGACGAGAAAGGCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGt                                                                                                                                            <  7:255209/149‑1 (MQ=255)           gcgGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCGGGGCAACGGTTc                                                                                                                                          <  6:206730/149‑1 (MQ=255)           gcgGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCGGGGCAACGGTTc                                                                                                                                          <  6:201713/149‑1 (MQ=255)             ggCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGa                                                                                                                                        <  8:291164/149‑1 (MQ=255)               ccGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGAcc                                                                                                                                      <  2:254735/149‑1 (MQ=255)                      cACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGcc                                                                                                                                <  8:81405/148‑1 (MQ=255)                       aCGGCCTCCGACGAGAAAGTCGCCGACTACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGATGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGccac                                                                                                                              <  4:257066/149‑1 (MQ=255)                            cTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTGGCCCCTGGCCACCCCCt                                                                                                                         >  6:93891/1‑149 (MQ=255)                             tCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTc                                                                                                                        >  7:336912/1‑149 (MQ=255)                               cgacgaGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTcc                                                                                                                       <  4:65687/148‑1 (MQ=255)                                          tCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCCCCCACTCCGACCGCGagca‑‑g                                                                                                         >  5:191941/1‑149 (MQ=255)                                            gCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCt                                                                                                       >  8:224315/1‑149 (MQ=255)                                                  tACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCt                                                                                                 >  3:152556/1‑149 (MQ=255)                                                     gAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGGAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCt                                                                                                 >  6:263024/1‑146 (MQ=255)                                                     gAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGGAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCt                                                                                                 <  5:263024/146‑1 (MQ=255)                                                     gAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCAc                                                                                              >  6:219754/1‑149 (MQ=255)                                                       gcgcagcgcCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCAcgg                                                                                            >  2:102976/1‑149 (MQ=255)                                                          cagcgcCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCAcggccg                                                                                         <  5:229368/149‑1 (MQ=255)                                                           agcgcCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCAcggccgg                                                                                        <  8:334147/149‑1 (MQ=255)                                                               ccTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGt                                                                                    <  7:47833/149‑1 (MQ=255)                                                               ccTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGt                                                                                    <  5:93891/149‑1 (MQ=255)                                                                         cTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTc                                                                                                <  1:92049/127‑1 (MQ=255)                                                                         cTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTc                                                                                                >  2:92049/1‑127 (MQ=255)                                                                            tgGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACcgc                                                                                                                 >  5:131451/1‑109 (MQ=255)                                                                            tgGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACcgc                                                                                                                 <  6:131451/109‑1 (MQ=255)                                                                              gTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGag                                                                                                              >  1:166200/1‑110 (MQ=255)                                                                              gTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGag                                                                                                              <  2:166200/110‑1 (MQ=255)                                                                               ttCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCtgt                                                                    >  4:7278/1‑149 (MQ=255)                                                                                tCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCtgtg                                                                   >  3:292964/1‑149 (MQ=255)                                                                                     cTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCACGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgc                                                              <  8:54651/149‑1 (MQ=255)                                                                                      tCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCACCGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgcg                                                             >  6:93865/1‑149 (MQ=255)                                                                                      tCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTc                                                                                                   <  3:181278/111‑1 (MQ=255)                                                                                      tCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTc                                                                                                   >  4:181278/1‑111 (MQ=255)                                                                                      tCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgcg                                                             >  2:160505/1‑149 (MQ=255)                                                                                      tCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACAGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTTTGCAAgcg                                                             >  2:155133/1‑149 (MQ=255)                                                                                       ccGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGCGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgcgc                                                            >  8:184837/1‑149 (MQ=255)                                                                                            aTGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGATGTGCAAGCGctacta                                                       >  1:61625/1‑149 (MQ=255)                                                                                                 cACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACTGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGc                                                   <  3:100247/148‑1 (MQ=255)                                                                                                    cGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCgaga                                               <  7:215654/149‑1 (MQ=255)                                                                                                       cAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCATCTCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGaa                                              <  1:102976/149‑1 (MQ=255)                                                                                                             gatgatCTGGCTGTCCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTTGCCAGCCACTCCGACCTCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGa                                        <  5:219754/147‑1 (MQ=255)                                                                                                             gATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgcgc                                                            >  6:259060/1‑127 (MQ=255)                                                                                                             gATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAgcgc                                                            <  5:259060/127‑1 (MQ=255)                                                                                                             gATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGt                                      >  6:40599/1‑149 (MQ=255)                                                                                                             gATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGt                                      >  5:52550/1‑149 (MQ=255)                                                                                                               tGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCg                                    <  4:292964/149‑1 (MQ=255)                                                                                                                  aCTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTTCGGCGAGAATGACGAGTCGGTc                                 <  7:224315/149‑1 (MQ=255)                                                                                                                  aCTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTc                                 >  8:165269/1‑149 (MQ=255)                                                                                                                      ccTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGGGTCGGTCCTg                              <  3:7278/148‑1 (MQ=255)                                                                                                                      ccTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGc                             >  7:252321/1‑149 (MQ=255)                                                                                                                         gACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCAc                          >  1:275132/1‑149 (MQ=255)                                                                                                                           ccGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACgc                        <  4:152556/149‑1 (MQ=255)                                                                                                                            cGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACg                         >  6:235041/1‑147 (MQ=255)                                                                                                                                 cccTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATc                  >  7:51306/1‑149 (MQ=255)                                                                                                                                      ggCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCaa             <  5:235041/149‑1 (MQ=255)                                                                                                                                         cTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCAACtt          <  8:252321/149‑1 (MQ=255)                                                                                                                                             cccTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCAACTTCaaa      <  7:165269/149‑1 (MQ=255)                                                                                                                                                  ccGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCAACTTCAAAGCGt  <  8:336912/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CATGGTCATCGCGGCCGACTCCACGGCCTCCGACGAGAAAGTCGCCGAATACGAGCGCAGCGCCTGCCCGACCTGTGGTTCGTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAAGCCCTGGGCCTGGCCCTGCCGGGCAACGGTTCGACCCTGGCCACCCACTCCGACCGCGAGCA‑‑GCTGTTCCTCACGGCCGGCCGTACCATCGTCGAGCTGTGCAAGCGCTACTACGGCGAGAATGACGAGTCGGTCCTGCCACGCAGCATCGCCAACTTCAAAGCGT  >  NC_002947/5849790‑5850082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑