Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,171 T→A 6.8% intergenic (+34/+34) ilvD → / ← PP_5129 dihydroxy‑acid dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,1710TA 6.8% 172.0 / 2.6 59intergenic (+34/+34)ilvD/PP_5129dihydroxy‑acid dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base A (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GCCAGCCGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCCTCC  >  NC_002947/5851023‑5851318
                                                                                                                                                    |                                                                                                                                                   
gccagccGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                     <  6:339642/149‑1 (MQ=255)
            ccgtccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                     >  7:270189/1‑137 (MQ=255)
            ccgtccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                     <  8:270189/137‑1 (MQ=255)
             cgtccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCAGGGCGCCGGttc                                                                                                                                         <  2:223190/148‑2 (MQ=255)
                 cACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGttttttt                                                                                                                                     <  2:113667/148‑1 (MQ=255)
                  aCGCAAGGTAACCACCGCGCTCAAGGCCTATTCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTg                                                                                                                                   <  5:187687/149‑1 (MQ=255)
                   cGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGa                                                                                                                                  <  8:323386/149‑1 (MQ=255)
                   cGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGa                                                                                                                                  <  7:185693/149‑1 (MQ=255)
                                ccGCGCTCAAGGCCTATGCCCTACTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACg                                                                                                                     >  7:117917/1‑149 (MQ=255)
                                   cgcTCAAGGCCTATGCCCTACTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTc                                                                                                                  <  8:117917/149‑1 (MQ=255)
                                      tCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGt                                                                                                               >  5:364328/1‑149 (MQ=255)
                                        aaGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGGAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTTATTGTTCATTGACGGTCAGTg                                                                                                              >  2:76546/1‑148 (MQ=255)
                                             cTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGa                                                                                                        <  4:150438/149‑1 (MQ=255)
                                              tATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGt                                                                                                               <  2:99333/141‑1 (MQ=255)
                                              tATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGAGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGt                                                                                                               >  1:99333/1‑141 (MQ=255)
                                                tGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGt                                                                                                     <  8:336254/149‑1 (MQ=255)
                                                  cccTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                     <  8:209922/99‑1 (MQ=255)
                                                  cccTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                     >  7:209922/1‑99 (MQ=255)
                                                   ccTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGc                                                                                                  >  3:301302/1‑149 (MQ=255)
                                                          gCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCg                                                                                           >  4:337478/1‑149 (MQ=255)
                                                          gCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCg                                                                                           >  2:224026/1‑149 (MQ=255)
                                                            caccaGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGa                                                                                         <  3:337478/149‑1 (MQ=255)
                                                              ccaGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATc                                                                                       <  1:224026/149‑1 (MQ=255)
                                                                    ccGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACa                                                                                 >  2:174006/1‑149 (MQ=255)
                                                                    ccGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACa                                                                                 <  6:364328/149‑1 (MQ=255)
                                                                     cGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAt                                                                                >  4:163996/1‑149 (MQ=255)
                                                                     cGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAt                                                                                <  3:183022/149‑1 (MQ=255)
                                                                         aaGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGAc                                                                            <  2:25563/149‑1 (MQ=255)
                                                                          aGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACa                                                                           <  6:33607/149‑1 (MQ=255)
                                                                           gggTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAg                                                                          >  8:110822/1‑149 (MQ=255)
                                                                                   tgcgTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGga                                                                  <  7:110822/149‑1 (MQ=255)
                                                                                        aaCAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGc                                                              >  1:126949/1‑148 (MQ=255)
                                                                                          cAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGAtt                                                                                                                                <  4:193816/80‑1 (MQ=255)
                                                                                          cAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGAtt                                                                                                                                >  3:193816/1‑80 (MQ=255)
                                                                                           aaGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGAtt                                                          >  1:179254/1‑149 (MQ=255)
                                                                                           aaGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGAtt                                                          >  7:177339/1‑149 (MQ=255)
                                                                                               cAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGGAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTTATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCaa                                                      <  1:76546/149‑1 (MQ=255)
                                                                                                aaTGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAg                                                     >  5:296578/1‑149 (MQ=255)
                                                                                                    cTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGc                                                  <  4:301302/148‑1 (MQ=255)
                                                                                                       gAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCg                                              <  1:174006/149‑1 (MQ=255)
                                                                                                         aGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGt                                             <  2:179254/148‑1 (MQ=255)
                                                                                                          ggCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGAAAGCTTTTGGAGAGCGATTCCAAGTGCGTCGTCa                                           >  3:211747/1‑149 (MQ=255)
                                                                                                           gCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCg                                              >  7:151251/1‑145 (MQ=255)
                                                                                                           gCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCg                                              <  8:151251/145‑1 (MQ=255)
                                                                                                            ccTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATc                                         <  2:126949/149‑1 (MQ=255)
                                                                                                             ctctGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATc                                         >  4:110551/1‑148 (MQ=255)
                                                                                                                    gTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGtt                                 <  3:110551/149‑1 (MQ=255)
                                                                                                                    gTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGtt                                 >  7:25153/1‑149 (MQ=255)
                                                                                                                      gattttATCTTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGttt                                <  4:211747/146‑1 (MQ=255)
                                                                                                                       cTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTg                               <  6:296578/148‑1 (MQ=255)
                                                                                                                        ttttAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCt                             >  3:169663/1‑149 (MQ=255)
                                                                                                                             gCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTg                                                                    <  6:349732/105‑1 (MQ=255)
                                                                                                                             gCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTg                                                                    >  5:349732/1‑105 (MQ=255)
                                                                                                                             gCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCttttt                         <  8:158871/148‑1 (MQ=255)
                                                                                                                               gTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAtt                                                                               <  2:292831/92‑1 (MQ=255)
                                                                                                                               gTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAtt                                                                               >  1:292831/1‑92 (MQ=255)
                                                                                                                                  aaaTAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGag                     >  8:45678/1‑147 (MQ=255)
                                                                                                                                      aaGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCgg               >  6:313248/1‑149 (MQ=255)
                                                                                                                                         aaCCGGCGCCCAGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTc                                            >  6:187513/1‑117 (MQ=255)
                                                                                                                                         aaCCGGCGCCCAGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTc                                            <  5:187513/117‑1 (MQ=255)
                                                                                                                                           ccGGCGCCCAGGGCGCCGGTTTTTTTTGTTTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGCTCGAAACATTGACAGCTTTTGGAGAGGGATTCCAAGTGCCTCGTCATCGAACAGTTTGGTTTTTCGAGCAGCGGCAcc           >  1:143243/1‑148 (MQ=255)
                                                                                                                                                  ccTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCcctc   >  7:37127/1‑149 (MQ=255)
                                                                                                                                                   cTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGTAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCcctcc  <  5:313248/149‑1 (MQ=255)
                                                                                                                                                   cTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCcctcc  <  3:163996/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCAGCCGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCCTCC  >  NC_002947/5851023‑5851318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑