Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,862,538 C→A 7.3% G84V (GGC→GTC)  fbpC ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,862,5380CA 7.3% 172.1 / 3.8 55G84V (GGC→GTC) fbpCABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base A (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

GCCAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAGGCACACCAGTTC  >  NC_002947/5862402‑5862683
                                                                                                                                        |                                                                                                                                                 
gCCAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCcgcg                                                                                                                                       >  2:309254/1‑149 (MQ=255)
 ccAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCgcgc                                                                                                                                      >  6:24651/1‑149 (MQ=255)
 ccAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCgcgc                                                                                                                                      >  4:134772/1‑149 (MQ=255)
 ccAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGACGTAGTCCCgcgc                                                                                                                                      >  4:24716/1‑149 (MQ=255)
       aGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTgggg                                                                                                                                <  8:244403/149‑1 (MQ=255)
       aGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTgggg                                                                                                                                <  3:302021/149‑1 (MQ=255)
         gCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTggggg                                                                                                                               >  4:144178/1‑148 (MQ=255)
           cTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGAc                                                                                                                            <  3:134772/149‑1 (MQ=255)
                 gTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCt                                                                                                                                    >  6:24928/1‑135 (MQ=255)
                 gTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCt                                                                                                                                    <  5:24928/135‑1 (MQ=255)
                  ttCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGa                                                                                                                             >  5:274656/1‑141 (MQ=255)
                  ttCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGa                                                                                                                             >  5:269708/1‑141 (MQ=255)
                  ttCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCgg                                                                                                                     <  7:300242/149‑1 (MQ=255)
                            tCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATcgc                                                                                                            <  5:24651/148‑1 (MQ=255)
                                   cccGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGcc                                                                                                     >  5:143332/1‑148 (MQ=255)
                                         ggCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGTCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCg                                                                                                                                                 <  1:269188/98‑1 (MQ=255)
                                         ggCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCg                                                                                                                                                 >  2:269188/1‑98 (MQ=255)
                                          gCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGTGGGACAGATCGGAGATATCGCGTTCTCCTATGTACa                                                                                             >  4:251252/1‑149 (MQ=255)
                                          gCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGACGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACa                                                                                             <  3:24716/149‑1 (MQ=255)
                                                      gcgACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGAt                                                                                 >  1:47859/1‑149 (MQ=255)
                                                        gACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGAGATcc                                                                               >  7:303007/1‑149 (MQ=255)
                                                         aCCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCt                                                                              <  5:293855/149‑1 (MQ=255)
                                                           gaCTGCCCGTCAGGCCATAGGCGATTTTGGCTTCGACGGTAAGGTTGGGAATCAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTgg                                                                            <  3:251252/148‑1 (MQ=255)
                                                           cACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTgg                                                                            >  6:192650/1‑149 (MQ=255)
                                                              tGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGgcgc                                                                         >  5:219376/1‑149 (MQ=255)
                                                                cccGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGgcgc                                                                         <  6:274656/147‑1 (MQ=255)
                                                                cccGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGGTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGgcgc                                                                         <  6:269708/147‑1 (MQ=255)
                                                                           aTAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCaa                                                            >  5:238723/1‑149 (MQ=255)
                                                                              ggCGATTTGCGCTTCGACGTTAACGTTGGGACACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATg                                                          <  6:252223/148‑1 (MQ=255)
                                                                              ggCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATg                                                          >  3:257969/1‑148 (MQ=255)
                                                                               gCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCa                                                        <  5:192650/149‑1 (MQ=255)
                                                                                cGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAAt                                                           >  6:3364/1‑145 (MQ=255)
                                                                                cGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAAt                                                           <  5:3364/145‑1 (MQ=255)
                                                                                        gCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTgg                                                                            >  7:305158/1‑120 (MQ=255)
                                                                                        gCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTgg                                                                            <  8:305158/120‑1 (MQ=255)
                                                                                          ttCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCg                                             >  1:251229/1‑149 (MQ=255)
                                                                                             gACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCATTGCAACGCAGCAGCGTgg                                          >  3:250069/1‑149 (MQ=255)
                                                                                             gACCGTAAGGTGGCGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTg                                           <  4:202143/148‑1 (MQ=255)
                                                                                                ggTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCt                                       >  1:222070/1‑149 (MQ=255)
                                                                                                 gTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCtt                                      >  7:177384/1‑149 (MQ=255)
                                                                                                  tAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTg                                     >  7:57698/1‑149 (MQ=255)
                                                                                                          ggAAACAGCGCGTAGGACTGGAACAGGATGACGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACg                                                     >  2:182634/1‑125 (MQ=255)
                                                                                                          ggAAACAGCGCGTAGGACTGGAACAGGATGACGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACg                                                     <  1:182634/125‑1 (MQ=255)
                                                                                                           gAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCg                            <  3:350796/149‑1 (MQ=255)
                                                                                                           gAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCg                            <  3:345161/149‑1 (MQ=255)
                                                                                                            aaaCAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCg                            >  8:213120/1‑148 (MQ=255)
                                                                                                                   gcgTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGcc                    >  2:58390/1‑149 (MQ=255)
                                                                                                                         gACTGGAACAGGTTGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAg              <  6:143332/149‑1 (MQ=255)
                                                                                                                         gACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAg              >  8:56626/1‑149 (MQ=255)
                                                                                                                         gACTGGAACAGGATGCCGTAGTCCCGCGCCT‑GGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATg                        >  5:27078/1‑138 (MQ=255)
                                                                                                                         gACTGGAACAGGATGCCGTAGTCCCGCGCCT‑GGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATg                        <  6:27078/138‑1 (MQ=255)
                                                                                                                          aCTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAgg             <  8:177384/149‑1 (MQ=255)
                                                                                                                                    gATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAGGCACACCAGtt   >  3:291584/1‑149 (MQ=255)
                                                                                                                                    gATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAGGCACACCAGtt   >  1:263326/1‑149 (MQ=255)
                                                                                                                                     aTGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAGGCACACCAGTTc  <  7:56626/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                 
GCCAGACAGGCCTACCAGTTCGAGCATTTCGGCAACCCGCTGGCGGGCCTGCTCGCGACCACTGCCCGTCAGGCCATAGGCGATATTGGCTTCGACGGTAAGGTTGGGAAACAGCGCGTAGGACTGGAACAGGATGCCGTAGTCCCGCGCCTGGGGGGACAGATCGGAGATATCGCGTTCGCCGATGTACAGCGTACCGCGATCCTGGCGCTCCAGGCCGGCAATGCAACGCAGCAGCGTGGTCTTGCCACAGCCGGATGGGCCGAGCAGGCACACCAGTTC  >  NC_002947/5862402‑5862683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑