Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,868,055	A→T	5.6%	L170* (TTG→TAG)	lgt ←	prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,868,055	0	A	T	5.6%	214.7 / 2.9	71	L170* (TTG→TAG)	lgt	prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base A (33/34);  new base T (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

AAGCGGAAGATGCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGAAGTCCATCAGCT  >  NC_002947/5867914‑5868199                                                                                                                                               |                                                                                                                                                  aaGCGGAAGATGCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGc                                                                                                                                            <  6:65822/149‑1 (MQ=255)  aGCGGAAGATGCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCGACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGcc                                                                                                                                           <  4:235839/149‑1 (MQ=255)       aaGATGCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGtt                                                                                                                                     <  4:256253/149‑2 (MQ=255)            gCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCGCCGAGACCTGCCAAAGGATGACCACCAGCGCCACGCCCACAAGGGGGAACTGGAAGACCGCCGATGGGTGGCGCGCCACCTGGGCC‑gggaggct                                                                                                                                  >  1:93636/1‑143 (MQ=255)            gCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTg                                                                                                                                 >  8:47160/1‑148 (MQ=255)             ccATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCTCGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGaa                                                                                                                               <  4:314577/149‑1 (MQ=255)             ccATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGaa                                                                                                                               >  1:160269/1‑149 (MQ=255)                  gCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAAcggcg                                                                                                                          <  2:354445/149‑1 (MQ=255)                     caACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCggg                                                                                                                        >  5:324464/1‑148 (MQ=255)                      aaCGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCggg                                                                                                                        >  6:102628/1‑147 (MQ=255)                        cGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTgg                                                                                                                                              <  4:217662/124‑1 (MQ=255)                        cGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTgg                                                                                                                                              >  3:217662/1‑124 (MQ=255)                            aaCATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAAcggcg                                                                                                                          <  8:167759/139‑1 (MQ=255)                            aaCATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAAcggcg                                                                                                                          >  7:167759/1‑139 (MQ=255)                               aTTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGc                                                                                                             >  6:164760/1‑149 (MQ=255)                                ttCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGcc                                                                                                            >  3:57450/1‑149 (MQ=255)                                     gACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATgg                                                                                                       <  5:102628/149‑1 (MQ=255)                                     gACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATgg                                                                                                       <  6:320220/149‑1 (MQ=255)                                        acCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGAc                                                                                                    >  8:20021/1‑149 (MQ=255)                                          cGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGt                                                                                                  >  1:184378/1‑149 (MQ=255)                                          cGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGt                                                                                                  <  2:160269/149‑1 (MQ=255)                                          cGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCCAACTGCAAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGt                                                                                                  >  2:254044/1‑149 (MQ=255)                                            ccATTGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATgg                                                                                                       <  6:166215/142‑1 (MQ=255)                                            ccATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATgg                                                                                                       >  5:166215/1‑142 (MQ=255)                                             cATGGTCGGCCGTGGCTTGCG‑CGAGAACAGNCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCgg                                                                                               >  1:33208/1‑149 (MQ=255)                                             cATGGTCGGCCGTGGCTTGCG‑CGAGAACAGACACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCgg                                                                                               >  1:27697/1‑149 (MQ=255)                                                 gTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTgg                                                                                            <  5:164760/148‑1 (MQ=255)                                                   cGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCGCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GCGTCGCTGAACCGCGGGAAGATCAACGCCCCTGGGACGACGGTGGGtt                                                                                         >  3:37523/1‑149 (MQ=255)                                                     gCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCCGGGGCCTCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGttt                                                                                        <  1:254044/149‑1 (MQ=255)                                                     gCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTg                                                                                       >  6:238456/1‑149 (MQ=255)                                                        gTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCTCGGCCTCCAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGGAACTGGGGC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGAGGTCGGTGGGTTTGccg                                                                                    <  6:63394/149‑2 (MQ=255)                                                                     agaACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGc                                                                                                                                   <  6:63974/90‑1 (MQ=255)                                                                      gaACAGGCACAGGATGACGACCAGCGCCACGCCCTCATGGGCGTACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGGGt                                                                       <  4:37523/149‑1 (MQ=255)                                                                          aGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTAGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGcc                                                                                     >  8:217347/1‑131 (MQ=255)                                                                          aGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTAGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGcc                                                                                     <  7:217347/131‑1 (MQ=255)                                                                           gCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTtgatg                                                                  <  5:238456/149‑1 (MQ=255)                                                                                 ggATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTg                                                            >  2:78659/1‑149 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGCGCTGCGATGGGTGGCGCGGCTACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGc                                                                                      >  5:260515/1‑120 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCTACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGc                                                                                      >  5:265365/1‑120 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCTACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGc                                                                                      <  6:260515/120‑1 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCTACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGc                                                                                      <  6:265365/120‑1 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCg                                                         <  2:33208/149‑1 (MQ=255)                                                                                    tGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGGTCGGCGTTGATGAAGTTGCCg                                                         <  2:27697/149‑1 (MQ=255)                                                                                      aCGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGAt                                                       <  7:20021/149‑1 (MQ=255)                                                                                          aCAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGc                                                   >  1:47240/1‑149 (MQ=255)                                                                                            aGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGccc                                                 >  3:123947/1‑149 (MQ=255)                                                                                             gcgcCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCg                                                >  2:22039/1‑149 (MQ=255)                                                                                                ccACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGcc                                             <  8:197593/149‑1 (MQ=255)                                                                                                 cACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGccc                                            >  1:105776/1‑149 (MQ=255)                                                                                                 cACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGccc                                            >  8:105975/1‑149 (MQ=255)                                                                                                  aCGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGa                                                        >  6:296638/1‑136 (MQ=255)                                                                                                  aCGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGa                                                        <  5:296638/136‑1 (MQ=255)                                                                                                      ccTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAgg                                       <  2:184378/149‑1 (MQ=255)                                                                                                       cTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGc                                      <  6:324464/149‑1 (MQ=255)                                                                                                          aaGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGt                                                                                                  <  7:164782/86‑1 (MQ=255)                                                                                                          aaGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGt                                                                                                  >  8:164782/1‑86 (MQ=255)                                                                                                              gCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGaa                                                                <  2:62214/116‑1 (MQ=255)                                                                                                              gCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGaa                                                                >  1:62214/1‑116 (MQ=255)                                                                                                              gCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGc                                      <  2:44017/142‑1 (MQ=255)                                                                                                              gCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGc                                      >  1:44017/1‑142 (MQ=255)                                                                                                               cGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGc                              <  2:47240/149‑1 (MQ=255)                                                                                                                      gTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGcc                                             <  3:239690/127‑1 (MQ=255)                                                                                                                      gTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGcc                                             >  4:239690/1‑127 (MQ=255)                                                                                                                           gCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGcg                  <  1:22039/149‑1 (MQ=255)                                                                                                                               cGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCCCCAGCGGGGcgacg               >  4:214533/1‑148 (MQ=255)                                                                                                                                gATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGaa             <  2:107878/149‑1 (MQ=255)                                                                                                                                    ggTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGAAGTc          <  7:47160/148‑1 (MQ=255)                                                                                                                                     gTGGCGCGCCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGGGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGACTATACGAACCACCGGGGCGACGAAGTcaa        >  7:335818/1‑147 (MQ=255)                                                                                                                                        gcgcGGCAACTGGGCC‑GGGTCGCTCAACGGCGGGAAGATCATCGCCCATGGGACGTCGCTGGGTATGCCCCACAGTTCGGCGTTGATGCAGTTGCCGATACGCCCGGCCCCCCGGCCAATCGGCACCAGCGGGGCGACGAAGTCCATCa     >  6:31171/1‑149 (MQ=255)                                                                                                                                         cgcgGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGAAGTCCATCAg    <  4:57450/149‑1 (MQ=255)                                                                                                                                           cgGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGAAGTCCATCAGCt  >  4:134146/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  AAGCGGAAGATGCCATAGCACAACGAGAACATTCCCGACACCGCCATGGTCGGCCGTGGCTTGCG‑CGAGAACAGCCACAGGATGACGAACAGCGCCACGCCCTCAAGGGCGAACTGGTAGAGCTGCGATGGGTGGCGCGGCAACTGGGCC‑GGGTCGCTGAACGGCGGGAAGATCATCGCCCATGGGACGTCGGTGGGTTTGCCCCACAGTTCGGCGTTGATGAAGTTGCCGATACGCCCGGCCCCCAGGCCAATCGGCACCAGCGGGGCGACGAAGTCCATCAGCT  >  NC_002947/5867914‑5868199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑