Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 410,834 A→G 8.0% intergenic (‑171/‑213) aceE ← / → glnE pyruvate dehydrogenase E1 component/glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947410,8340AG 8.0% 138.9 / 2.5 50intergenic (‑171/‑213)aceE/glnEpyruvate dehydrogenase E1 component/glutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base G (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGCCTGAGATTGCGACTGGCCTTATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACTTTTGTACGACAGTC  >  NC_002947/410698‑410980
                                                                                                                                        |                                                                                                                                                  
aTTGCCTGAGATTGCGACTGGCCTTATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGc                                                                                                                                        >  6:63547/1‑149 (MQ=255)
          aTTGCGACTGGCCTTATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGt                                                                                                                              <  5:63547/149‑1 (MQ=255)
               gACTGGCCTTATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCAc                                                                                                                         >  7:124937/1‑149 (MQ=255)
                       ttATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCtt                                                                                                                 >  5:81685/1‑149 (MQ=255)
                          tGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTTTGCGACGTTTGGTCGCACCTTGGCTtgt                                                                                                               >  7:318303/1‑148 (MQ=255)
                           gggCATACGTCGTTAATTCTTGGAATACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGa                                                                                                             <  7:259485/149‑1 (MQ=255)
                            ggCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTaaaa                                                                                                                                                    >  1:7747/1‑109 (MQ=255)
                            ggCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTaaaa                                                                                                                                                    <  2:7747/109‑1 (MQ=255)
                            ggCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGa                                                                                                             <  8:124937/148‑1 (MQ=255)
                            ggCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAg                                                                                                            <  2:237531/149‑1 (MQ=255)
                                                ggATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttg                                                                                           >  4:230890/1‑146 (MQ=255)
                                                ggATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttg                                                                                           <  3:230890/146‑1 (MQ=255)
                                                 gATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTgg                                                                                                                               >  1:312615/1‑109 (MQ=255)
                                                 gATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTgg                                                                                                                               <  2:312615/109‑1 (MQ=255)
                                                     aCCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCa                                                                                    <  3:341508/148‑1 (MQ=255)
                                                      ccGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAg                                                                                                            >  4:42180/1‑123 (MQ=255)
                                                         gggTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttg                                                                                           <  2:194061/137‑1 (MQ=255)
                                                         gggTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttg                                                                                           >  1:194061/1‑137 (MQ=255)
                                                               cTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttggttg                                                                                       >  8:334214/1‑135 (MQ=255)
                                                               cTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATgttggttg                                                                                       <  7:334214/135‑1 (MQ=255)
                                                               cTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTcgcg                                                                          >  1:179281/1‑148 (MQ=255)
                                                                ttCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGaa                                                                        >  2:282762/1‑149 (MQ=255)
                                                                ttCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGaa                                                                        >  6:91302/1‑149 (MQ=255)
                                                                  ccGGCGCGTTGTAGTTTTTCTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGTCGTTTGGTCGCACCTTGGCTTGTGATGAAAAACGTTCTTGTTGGTTGGCATTGTGTCGCGAAc                                                                       <  1:139679/148‑1 (MQ=255)
                                                                       gcgcTGTACGTTTACTACATATGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGGGAGGAAAAACGTTCATGTTGGTTGGCATTGTGGCGCGAACGggtgg                                                                 <  6:81685/149‑1 (MQ=255)
                                                                           tGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCt                                                             >  2:125265/1‑149 (MQ=255)
                                                                            gTAGTTTTACTAAATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCt                                                             <  1:282762/148‑1 (MQ=255)
                                                                              aGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTg                                                           <  1:125265/148‑1 (MQ=255)
                                                                               gTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGa                                                          <  6:318754/148‑1 (MQ=255)
                                                                                       acATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTttgttg                                                 >  3:157839/1‑149 (MQ=255)
                                                                                              cTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAAtt                                           <  7:199248/148‑1 (MQ=255)
                                                                                               tttCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCt                                                             >  1:155465/1‑129 (MQ=255)
                                                                                               tttCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCt                                                             <  2:155465/129‑1 (MQ=255)
                                                                                                  cGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTc                                       <  4:157839/148‑1 (MQ=255)
                                                                                                   gCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGt                                     >  7:183699/1‑149 (MQ=255)
                                                                                                          aTGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGAtgt                                                       >  7:104999/1‑124 (MQ=255)
                                                                                                          aTGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGAtgt                                                       <  8:104999/124‑1 (MQ=255)
                                                                                                             cTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAg                            <  5:91302/148‑1 (MQ=255)
                                                                                                             cTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAg                            <  2:179281/148‑1 (MQ=255)
                                                                                                                         acgaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACtt               >  2:109907/1‑149 (MQ=255)
                                                                                                                           gaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAATCGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCaa                  <  6:246433/144‑1 (MQ=255)
                                                                                                                           gaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAATCGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCaa                  >  5:246433/1‑144 (MQ=255)
                                                                                                                           gaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTAt                        <  3:17355/138‑1 (MQ=255)
                                                                                                                           gaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTAt                        >  4:17355/1‑138 (MQ=255)
                                                                                                                           gaGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACtttt             <  1:109907/149‑1 (MQ=255)
                                                                                                                                gtagtaAAACTACATTTGTGCGACGTTTGGTGGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGAtgtttgtt                                                  >  5:108866/1‑107 (MQ=255)
                                                                                                                                gtagtaAAACTACATTTGTGCGACGTTTGGTGGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGAtgtttgtt                                                  <  6:108866/107‑1 (MQ=255)
                                                                                                                                gtagtaAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAg                            >  8:337154/1‑129 (MQ=255)
                                                                                                                                   gtaAAGCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGtt                                    <  7:129641/118‑1 (MQ=255)
                                                                                                                                   gtaAAGCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGtt                                    <  7:124750/118‑1 (MQ=255)
                                                                                                                                   gtaAAGCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGtt                                    >  8:124750/1‑118 (MQ=255)
                                                                                                                                   gtaAAGCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGtt                                    >  8:129641/1‑118 (MQ=255)
                                                                                                                                      aaaCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACTTTTGTACGACAGTc  >  4:135400/1‑149 (MQ=255)
                                                                                                                                       aaCTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACTTTTGTACGACAGTc  >  8:184338/1‑148 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
ATTGCCTGAGATTGCGACTGGCCTTATGGGCAGACGTCGTGAATTCTTGGATTACCGGGGTGTCTTCCGGCGCGTTGTAGTTTTACTACATTTGCTTTCGCATTTCATGCTTTTACACGACACGAGTAGTAGTAAAACTACATTTGTGCGACGTTTGGTCGCACCTTGGCTTGTGAGGAAAAACGTTCATGTTGGTTGGCATTGTGTCGCGAACGGGTGGTTCTTGATGTTTGTTGCCAATTTTTCGTTTTTTTTAGCTATTTCCAACTTTTGTACGACAGTC  >  NC_002947/410698‑410980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑