Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,891,213 G→T 7.7% G135G (GGC→GGA) ‡ plpB ← NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,891,2130GT 7.7% 201.0 / 6.0 66G135G (GGC→GGA) ‡plpBNLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (33/27);  new base T (2/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CAGCACACGGGGCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAA  >  NC_002947/5891069‑5891359
                                                                                                                                                |                                                                                                                                                  
cAGCACACGGGGCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGc                                                                                                                                                >  6:74530/1‑149 (MQ=255)
cAGCACACGGGGCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGc                                                                                                                                                >  6:80928/1‑149 (MQ=255)
    acacGGGGCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGtt                                                                                                                                            >  2:127765/1‑149 (MQ=255)
         gggCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAACAGCGGCCGTGCGCtgctgctgc                                                                                                                                       <  5:185986/149‑1 (MQ=255)
          ggCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCAGCTGTTGCTGcc                                                                                                                                      >  7:93268/1‑149 (MQ=255)
           gcagcaTGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAACAGCGGCCGTGCGCtgctgctgctg                                                                                                                                     <  1:230159/149‑3 (MQ=255)
            cagcaTGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCt                                                                                                                                    >  7:212860/1‑149 (MQ=255)
                   gCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAg                                                                                                                              <  1:127765/148‑1 (MQ=255)
                   gCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTAGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAgg                                                                                                                             <  8:284469/149‑1 (MQ=255)
                                 gcgGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTg                                                                                                               >  2:210502/1‑149 (MQ=255)
                                   ggAACTTCAGCTTCCTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTgcg                                                                                                             >  4:244512/1‑149 (MQ=255)
                                          cAGCTTCTTCGGGTTCTCGGGGATATCCTTGGAGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCcttctt                                                                                                      >  8:155125/1‑149 (MQ=255)
                                           aGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCttt                                                                                                     <  8:212860/149‑1 (MQ=255)
                                                cttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCCGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAg                                                                                                  <  5:80928/147‑1 (MQ=255)
                                                cttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGGTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAgc                                                                                                 <  3:348007/148‑1 (MQ=255)
                                                cttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAgc                                                                                                 <  5:74530/148‑1 (MQ=255)
                                                cttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAg                                                                                                  <  1:210502/147‑1 (MQ=255)
                                                 ttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGcc                                                                                                                                                  >  7:85240/1‑98 (MQ=255)
                                                 ttcGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGcc                                                                                                                                                  <  8:85240/98‑1 (MQ=255)
                                                   cGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAgctcgc                                                                                             >  7:320201/1‑149 (MQ=255)
                                                       ttCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAACAGCGGCCGTGCGCtgctgc                                                                                                                                          >  1:131968/1‑100 (MQ=38)
                                                       ttCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAACAGCGGCCGTGCGCtgctgc                                                                                                                                          <  2:131968/100‑1 (MQ=38)
                                                                          gggTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAg                                                                      <  7:155125/149‑1 (MQ=255)
                                                                             tGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCT‑‑‑GCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCa                                                                >  3:146334/1‑149 (MQ=255)
                                                                                ccAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCttt                                                                                                     <  5:139963/112‑1 (MQ=255)
                                                                                ccAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCttt                                                                                                     >  6:139963/1‑112 (MQ=255)
                                                                                  aGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCAcc                                                              >  3:14615/1‑149 (MQ=255)
                                                                                    ggCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCttt                                                                                                     >  8:176210/1‑108 (MQ=255)
                                                                                    ggCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCttt                                                                                                     <  7:176210/108‑1 (MQ=255)
                                                                                     gCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGaa                                                           <  2:75225/149‑1 (MQ=255)
                                                                                     gCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGaa                                                           <  8:320201/149‑1 (MQ=255)
                                                                                     gCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGACCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGaa                                                           <  1:165336/149‑1 (MQ=255)
                                                                                             cGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAggg                                                        >  4:299439/1‑144 (MQ=255)
                                                                                             cGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAggg                                                        <  3:299439/144‑1 (MQ=255)
                                                                                               ggTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCttcttc                                                                         >  8:302261/1‑125 (MQ=255)
                                                                                               ggTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCttcttc                                                                         <  7:302261/125‑1 (MQ=255)
                                                                                                   tttCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTc                                                                                                    >  6:192931/1‑94 (MQ=255)
                                                                                                   tttCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTc                                                                                                    <  5:192931/94‑1 (MQ=255)
                                                                                                   tttCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTa                                             >  4:86578/1‑149 (MQ=255)
                                                                                                     tCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTaca                                           >  3:58966/1‑149 (MQ=255)
                                                                                                     tCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTAGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACgtgt                                              <  4:14615/146‑1 (MQ=255)
                                                                                                      cAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTAc                                                  <  2:167433/141‑1 (MQ=255)
                                                                                                      cAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTAc                                                  >  1:167433/1‑141 (MQ=255)
                                                                                                       aaGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACgt                                                <  4:190839/142‑1 (MQ=255)
                                                                                                       aaGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACgt                                                >  3:190839/1‑142 (MQ=255)
                                                                                                         ggTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTAcaccca                                       >  4:115621/1‑149 (MQ=255)
                                                                                                                aGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGt                                >  6:186904/1‑149 (MQ=255)
                                                                                                                aGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCATTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGt                                >  1:89099/1‑149 (MQ=255)
                                                                                                                 ggccggccTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGtt                               >  3:63625/1‑149 (MQ=255)
                                                                                                                    cccccctCTGCAGCAGCAGCAGCGCACCGCCGCTGTTGCTGCCCTCCGTAGGGATCGCAACGGTTGCGCCATCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGACGGGCTCTACGTGTACACCCACCACGGCTTcc                            <  4:63625/143‑1 (MQ=255)
                                                                                                                        cTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggt                        <  3:244512/149‑1 (MQ=255)
                                                                                                                           cTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggtgg                      <  5:85049/148‑1 (MQ=255)
                                                                                                                            tGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGAc                                                                                <  1:6941/89‑1 (MQ=255)
                                                                                                                            tGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGAc                                                                                >  2:6941/1‑89 (MQ=255)
                                                                                                                                gcagcagcagcGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGccc                >  1:305468/1‑149 (MQ=255)
                                                                                                                                  agcaacagcGGCCGTGCGCTGCTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCtt              >  1:49410/1‑149 (MQ=255)
                                                                                                                                    cagcagcGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGc            >  6:356987/1‑149 (MQ=255)
                                                                                                                                        agcGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTc        >  2:279735/1‑149 (MQ=255)
                                                                                                                                         gcgcACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTc        >  6:292478/1‑148 (MQ=255)
                                                                                                                                         gcgcACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTc        <  5:292478/148‑1 (MQ=255)
                                                                                                                                         gcgcACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCg       >  2:351249/1‑149 (MQ=255)
                                                                                                                                           gcACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGGCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGAGGGTGCCCTTGCCTTCGtt     >  1:251803/1‑149 (MQ=255)
                                                                                                                                           gcACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGtt     <  5:356987/149‑1 (MQ=255)
                                                                                                                                            cACGGCCTCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTAc                                                  <  5:95233/103‑1 (MQ=255)
                                                                                                                                            cACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTAc                                                  >  6:95233/1‑103 (MQ=255)
                                                                                                                                              cGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGaa  <  5:186904/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CAGCACACGGGGCAGCATGGCCGACTCAAGCTCGCGGAACTTCAGCTTCTTCGGGTTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAA  >  NC_002947/5891069‑5891359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑