Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,899,958 C→G 5.3% Q310H (CAG→CAC)  PP_5173 ← RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,899,9580CG 5.3% 247.7 / 3.3 76Q310H (CAG→CAC) PP_5173RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (39/33);  new base G (2/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

CAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGATA  >  NC_002947/5899816‑5900105
                                                                                                                                              |                                                                                                                                                     
cAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAGGTGGTcc                                                                                                                                                 <  6:187224/149‑1 (MQ=255)
cAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTc                                                                                                                                                  >  6:342185/1‑148 (MQ=255)
 aaGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCa                                                                                                                                                >  3:172812/1‑149 (MQ=255)
  aGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAGGTGGTCCag                                                                                                                                               <  6:245965/149‑2 (MQ=255)
   gACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCGGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACGCACCGGCAGGTCCTGGACCACCTGGTCCATg                                                                                                                                              <  1:38105/149‑1 (MQ=255)
      tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGTTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATgcgc                                                                                                                                           >  7:300977/1‑149 (MQ=255)
      tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATgcgc                                                                                                                                           >  8:29444/1‑149 (MQ=255)
      tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATgcgc                                                                                                                                           >  8:38858/1‑149 (MQ=255)
        aCAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTg                                                                                                                                                     >  1:210212/1‑137 (MQ=255)
        aCAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTg                                                                                                                                                     <  2:210212/137‑1 (MQ=255)
        aCAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCtt                                                                                                                                         >  7:170928/1‑149 (MQ=255)
            aGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCtttt                                                                                                                                       <  8:163073/147‑1 (MQ=255)
            aGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCtttt                                                                                                                                       >  7:163073/1‑147 (MQ=255)
              cTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCaa                                                                                                                                   <  8:170928/149‑1 (MQ=255)
               tCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCaa                                                                                                                                   >  4:94183/1‑148 (MQ=255)
               tCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGGCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCCTGCGCTTTTTCAAc                                                                                                                                  >  4:42736/1‑149 (MQ=255)
                 aCGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCaa                                                                                                                                   <  3:94183/146‑1 (MQ=255)
                   ggCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAAcgccg                                                                                                                              >  4:133975/1‑149 (MQ=255)
                      cAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCttt                                                                                                                                        <  6:142741/136‑1 (MQ=255)
                      cAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCttt                                                                                                                                        >  5:142741/1‑136 (MQ=255)
                         cACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCg                                                                                                                         >  4:116978/1‑148 (MQ=255)
                                 ccaccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑gg                                                                                                               >  2:138145/1‑149 (MQ=255)
                                 ccaccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑gg                                                                                                               <  7:38858/149‑1 (MQ=255)
                                  caccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGa                                                                                                              <  4:137715/149‑1 (MQ=255)
                                   accGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGAt                                                                                                             >  8:83365/1‑149 (MQ=255)
                                        cTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTgc                                                                                                        >  1:313610/1‑149 (MQ=255)
                                                aggcggTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGcc                                                                                               <  1:35076/147‑1 (MQ=255)
                                                   cgcTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCtt                                                                                             >  8:93089/1‑148 (MQ=255)
                                                   cgcTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTc                                                                                            <  2:313610/149‑1 (MQ=255)
                                                       ggTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGc                                                                                        >  3:226005/1‑149 (MQ=255)
                                                        gTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGc                                                                                        <  7:83365/148‑1 (MQ=255)
                                                         tGAAACAGCCTACCGCCTGCGTGACCACCACGGCCTGGTCACAACCGGTTTGTACACCGACACCCACCGGCATGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGGTGCCGCC‑GGCCTTCATGCCg                                                                                      <  8:75136/149‑1 (MQ=255)
                                                           aaaCCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGAt                                                                                    >  1:114605/1‑149 (MQ=255)
                                                             accgcctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATgg                                                                                  <  7:29444/149‑1 (MQ=255)
                                                              ccgcctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGc                                                                                 >  4:296451/1‑149 (MQ=255)
                                                               cgcctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCAAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCg                                                                                >  1:149422/1‑149 (MQ=255)
                                                                 cctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAg                                                                              <  7:93089/149‑1 (MQ=255)
                                                                  ctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAgg                                                                             <  3:116978/149‑1 (MQ=255)
                                                                   taccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCAGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGc                                                                            >  2:16195/1‑149 (MQ=255)
                                                                     ccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGc                                                                            <  3:42736/147‑1 (MQ=255)
                                                                       gcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAAt                                                                        <  5:342185/149‑1 (MQ=255)
                                                                           gCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCt                                                                    >  2:30308/1‑149 (MQ=255)
                                                                            cTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCt                                                                    <  2:114605/148‑1 (MQ=255)
                                                                            cTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCAAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCt                                                                    <  2:149422/148‑1 (MQ=255)
                                                                                    ccacGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATg                                                                       <  3:134911/137‑1 (MQ=255)
                                                                                    ccacGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATg                                                                       >  4:134911/1‑137 (MQ=255)
                                                                                     cacGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGc                                                             <  1:289171/146‑1 (MQ=255)
                                                                                     cacGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGc                                                             >  2:289171/1‑146 (MQ=255)
                                                                                           cTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAAc                                                    <  2:258041/149‑1 (MQ=255)
                                                                                              gTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACgg                                                  >  7:2338/1‑148 (MQ=255)
                                                                                                cAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAAc                                               <  1:138145/149‑1 (MQ=255)
                                                                                                  gAAACGGTTTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCCGGGCCTTCATGCCGATGGCGAGGCCAATgg                                                                      <  1:197547/125‑1 (MQ=255)
                                                                                                              acacCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCgg                                 >  1:86526/1‑149 (MQ=255)
                                                                                                                   gACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGa                            <  1:30308/149‑1 (MQ=255)
                                                                                                                   gACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGa                            >  3:236618/1‑149 (MQ=255)
                                                                                                                      acccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACg                          >  5:224207/1‑148 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGc                                         >  3:86037/1‑131 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGc                                         <  4:86037/131‑1 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAg                       >  1:2196/1‑149 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAg                       >  3:276761/1‑149 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAg                       >  1:237999/1‑149 (MQ=255)
                                                                                                                        ccaccGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAg                       >  3:96718/1‑149 (MQ=255)
                                                                                                                          accGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGc                                         >  1:347351/1‑129 (MQ=255)
                                                                                                                          accGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGc                                         <  2:347351/129‑1 (MQ=255)
                                                                                                                               cAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCt                <  7:201087/149‑1 (MQ=255)
                                                                                                                                  gTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGa                                                 <  3:307823/113‑1 (MQ=255)
                                                                                                                                  gTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGa                                                 >  4:307823/1‑113 (MQ=255)
                                                                                                                                   tCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTaa                                                     <  7:6238/108‑1 (MQ=255)
                                                                                                                                   tCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTaa                                                     >  8:6238/1‑108 (MQ=255)
                                                                                                                                   tCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGAt            <  3:133975/149‑1 (MQ=255)
                                                                                                                                   tCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCCAt            >  1:134565/1‑149 (MQ=255)
                                                                                                                                   gcatGGACCAGGTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTAGGATGTTGCCGCC‑GGCGTTCATGCCTATGGCGCGGCCAATGGGTGTCTGCCCTTTGTAACGGAACAGTGCCGAAGGCGGGTCGACGTAGCCGCGCTCGa             >  1:84724/4‑149 (MQ=255)
                                                                                                                                   gcatGGACCAGGTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGAt            >  2:227391/4‑149 (MQ=255)
                                                                                                                                       ggACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCg        >  4:20007/1‑149 (MQ=255)
                                                                                                                                        gACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTc         <  6:224207/147‑1 (MQ=255)
                                                                                                                                        gACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCg        <  3:20007/148‑1 (MQ=255)
                                                                                                                                         accaccTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGc      <  3:296451/149‑1 (MQ=255)
                                                                                                                                             ccTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGata  >  7:129131/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCT‑GGATGTTGCCGCC‑GGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGATA  >  NC_002947/5899816‑5900105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑