Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,930,172 G→A 6.0% G72G (GGC→GGT)  visC ← ubiquinone biosynthesis oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,930,1720GA 6.0% 199.0 / 2.4 66G72G (GGC→GGT) visCubiquinone biosynthesis oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base A (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CCAGCAGGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGG  >  NC_002947/5930024‑5930316
                                                                                                                                                    |                                                                                                                                                
ccAGCAGGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGACAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATg                                                                                                                                                  <  8:197448/149‑1 (MQ=255)
 cagcagGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGc                                                                                                                                                 >  1:310685/1‑149 (MQ=255)
   gcagGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCg                                                                                                                                               >  4:118000/1‑149 (MQ=255)
   gcagGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCg                                                                                                                                               <  3:157481/149‑1 (MQ=255)
     agGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGATCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTc                                                                                                                                             <  4:80088/149‑1 (MQ=255)
       gCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTccc                                                                                                                                           >  8:153200/1‑149 (MQ=255)
           tCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGgc                                                                                                                                       <  5:340587/149‑1 (MQ=255)
             cTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGgcgc                                                                                                                                     <  3:118000/149‑1 (MQ=255)
                     cccGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGgcgc                                                                                                                             <  7:97085/149‑1 (MQ=255)
                      ccGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCt                                                                                                                            >  6:51622/1‑149 (MQ=255)
                         gTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGa                                                                                                                         <  7:153200/149‑1 (MQ=255)
                             tcGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGAt                                                                                                                     >  1:93210/1‑149 (MQ=255)
                               gacgaTATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATgc                                                                                                                   >  7:332909/1‑149 (MQ=255)
                                      tGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCCCACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATACCGTCCCAGGCGCCAAGGCGCTCGAGGATGCgctggct                                                                                                            >  3:187868/1‑149 (MQ=255)
                                      tGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATACCGTCCCAGGCGCCAAGGCGCTCGAGGATGCgctggct                                                                                                            >  3:192547/1‑149 (MQ=255)
                                           gAGCACCTGGGCATGCACACTGACCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGgccg                                                                                                       >  6:101966/1‑149 (MQ=255)
                                             gCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGgccgcc                                                                                                     <  5:51622/149‑1 (MQ=255)
                                                ccTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGa                                                                                                                         <  2:335734/126‑1 (MQ=255)
                                                ccTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGa                                                                                                                         >  1:335734/1‑126 (MQ=255)
                                                ccTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGa                                                                                                   >  3:152010/1‑148 (MQ=255)
                                                 cTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGgccccgttc                                                                                                  >  1:229342/1‑142 (MQ=255)
                                                  cgagcttGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAg                                                                                                <  5:322744/143‑1 (MQ=255)
                                                         gCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAg                                                                                                >  6:322744/1‑142 (MQ=255)
                                                                   cgccgAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCt                                                                               >  1:18267/1‑149 (MQ=255)
                                                                        agaAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCACTGCCGTCCCAGGCGACAAGGCGCTCGAGGATGCGCTTGCTGGCCGCCGCTAGCGAGCTGACCAGCGGCGAGAAc                                                                          >  6:356800/1‑149 (MQ=255)
                                                                         gaAGTGAATCTGCCCGGTGCCGCTGCCATCCCCCACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACg                                                                         >  8:173054/1‑149 (MQ=255)
                                                                          aaGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACgg                                                                        <  5:101966/149‑1 (MQ=255)
                                                                             tGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCgctggct                                                                                                            <  4:205214/110‑1 (MQ=255)
                                                                             tGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCgctggct                                                                                                            >  3:205214/1‑110 (MQ=255)
                                                                               aaTCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGcc                                                                    <  4:45081/148‑1 (MQ=255)
                                                                                     cccGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTgg                                                                 <  2:93210/145‑1 (MQ=255)
                                                                                     cccGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCAt                                                             >  6:61978/1‑149 (MQ=255)
                                                                                     cccGGGGCCGCTGCCAGCCCACCCCTGCCTGTCGGACTCCGTCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCGCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCGGACACGCGGCTCGAACGGGGCCTgg                                                                 <  5:356800/145‑1 (MQ=255)
                                                                                      ccGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATc                                                            >  4:158542/1‑149 (MQ=255)
                                                                                       cGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATc                                                            >  3:180249/1‑148 (MQ=255)
                                                                                         gTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTc                                                                              >  2:289145/1‑128 (MQ=255)
                                                                                         gTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTc                                                                              <  1:289145/128‑1 (MQ=255)
                                                                                         gTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGaa                                                         <  2:310685/149‑1 (MQ=255)
                                                                                          tGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAg                                                        <  2:18267/149‑1 (MQ=255)
                                                                                              gCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACggg                                                                       >  4:337847/1‑130 (MQ=255)
                                                                                              gCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACggg                                                                       <  3:204192/130‑1 (MQ=255)
                                                                                              gCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACggg                                                                       >  4:204192/1‑130 (MQ=255)
                                                                                              gCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACggg                                                                       <  3:337847/130‑1 (MQ=255)
                                                                                              gCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTa                                                    >  2:60179/1‑149 (MQ=255)
                                                                                                        cacacaTGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAg                                          >  5:280945/1‑149 (MQ=255)
                                                                                                             atgcatgTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGc                                     >  7:173393/1‑149 (MQ=255)
                                                                                                             atgcatgTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGc                                     <  4:152010/149‑1 (MQ=255)
                                                                                                                catgTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGcca                                   <  5:61978/148‑1 (MQ=255)
                                                                                                                    tCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATc                              >  8:293951/1‑149 (MQ=255)
                                                                                                                    tCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATc                              <  1:160542/149‑1 (MQ=255)
                                                                                                                       gAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAg                           <  4:180249/149‑1 (MQ=255)
                                                                                                                       gAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAg                           >  1:284022/1‑149 (MQ=255)
                                                                                                                              gCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACaaa                    <  6:280945/149‑1 (MQ=255)
                                                                                                                                     gcgcCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCa             <  3:158542/149‑1 (MQ=255)
                                                                                                                                     gcgcCGCTGGGCAATACCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCa             <  4:192547/149‑1 (MQ=255)
                                                                                                                                     gcgcCGCTGGGCAATACCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGGCAGCGGGCCACCATCGAGCAACAAAATTTGCa             <  4:187868/149‑1 (MQ=255)
                                                                                                                                         cgcTCGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTCGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGc          >  4:295992/1‑148 (MQ=255)
                                                                                                                                          gcTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCa        >  5:330205/1‑149 (MQ=255)
                                                                                                                                           cTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCa             >  7:278516/1‑143 (MQ=255)
                                                                                                                                           cTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCa             <  8:278516/143‑1 (MQ=255)
                                                                                                                                             gggCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACtg     >  8:264682/1‑149 (MQ=255)
                                                                                                                                               gCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACtgtg   >  4:150781/1‑149 (MQ=255)
                                                                                                                                                cAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCg                                        <  7:87647/111‑1 (MQ=255)
                                                                                                                                                cAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCg                                        >  8:87647/1‑111 (MQ=255)
                                                                                                                                                cAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTgg  <  7:293951/149‑1 (MQ=255)
                                                                                                                                                   tGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGccac                                  <  6:213703/114‑1 (MQ=255)
                                                                                                                                                   tGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGGACGCCCGTCAGCGGGccac                                  >  5:213703/1‑114 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCAGCAGGCCGTCCTGCACCACCCGGTTCTCGACGATATGGCCGAGCACCTGGGCATGCACACTGGCCGCCGAGAAGTGAATCTGCCCGGTGCCGCTGCCATCCCACACATGCATGTCGGAATACGGCGTGGCGCGCCGCTGGGCAATGCCGTCCCAGGCGCCAAGGCGCTCGAGGATGCGCTGGCTGGCCGCCGATAGCGCGCTGACACGCGGCTCGAACGGGGCCTGGGCATCGAAGGGTACGACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGG  >  NC_002947/5930024‑5930316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑