Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 417,306 A→T 7.4% Q339L (CAG→CTG)  waaG → lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947417,3060AT 7.4% 155.3 / 2.8 54Q339L (CAG→CTG) waaGlipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CGTTGGTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGG  >  NC_002947/417159‑417451
                                                                                                                                                   |                                                                                                                                                 
cGTTGGTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAg                                                                                                                                                  <  7:70073/149‑1 (MQ=255)
  ttGGTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGgc                                                                                                                                                <  8:201206/149‑1 (MQ=255)
     gTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGcgcg                                                                                                                                             >  8:183011/1‑149 (MQ=255)
      tCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGt                                                                                                                                            >  6:23629/1‑149 (MQ=255)
      tCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGt                                                                                                                                            <  8:333652/149‑1 (MQ=255)
          cGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCa                                                                                                                                        <  7:183011/149‑1 (MQ=255)
                   ggTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTcgc                                                                                                                               <  6:216697/149‑1 (MQ=255)
                        tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                          >  1:218119/1‑149 (MQ=255)
                        tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                          >  8:71507/1‑149 (MQ=255)
                          gtgtCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATgg                                                                                                                        >  3:64936/1‑149 (MQ=255)
                                      tgCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGc                                                                                                            >  4:304703/1‑149 (MQ=255)
                                        cGGTTATGCCCACTACATTGCCGAGGCCGACAGCTGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTTCCAGCTGGTCGCGCAATGGCCTGGCATTTGCt                                                                                                           <  3:263394/148‑1 (MQ=255)
                                         ggTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGa                                                                                                         <  7:71507/149‑1 (MQ=255)
                                           ttATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATa                                                                                                       <  3:123146/149‑1 (MQ=255)
                                                 ccACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGcc                                                                                                  >  1:250589/1‑148 (MQ=255)
                                                   aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGa                                                                                                >  3:191911/1‑148 (MQ=255)
                                                   aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGAt                                                                                               <  2:88683/149‑1 (MQ=255)
                                                    cTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATc                                                                                              >  6:308612/1‑149 (MQ=255)
                                                        aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                  <  8:125649/125‑1 (MQ=255)
                                                        aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                  >  7:125649/1‑125 (MQ=255)
                                                        aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTa                                                                                          <  2:218119/149‑1 (MQ=255)
                                                           gCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACa                                                                                        <  1:243912/148‑1 (MQ=255)
                                                              gAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCAt                                                                                    >  1:148218/1‑149 (MQ=255)
                                                                   cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgc                                                                               >  4:248031/1‑149 (MQ=255)
                                                                   cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCg                                                                                >  6:237095/1‑148 (MQ=255)
                                                                       aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                    >  5:154557/1‑124 (MQ=255)
                                                                       aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                    <  6:154557/124‑1 (MQ=255)
                                                                        gCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAt                                                                          <  2:250589/149‑1 (MQ=255)
                                                                              tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                      >  8:326191/1‑147 (MQ=255)
                                                                              tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                      <  7:326191/147‑1 (MQ=255)
                                                                                 tGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCACAGCATGCTGCCGAc                                                                 <  5:153217/149‑1 (MQ=255)
                                                                                           gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGCCCCGCAGGCGCGTGCCCGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGAACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                       >  3:20207/1‑149 (MQ=255)
                                                                                           gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                    <  8:211476/104‑1 (MQ=255)
                                                                                           gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                    >  7:211476/1‑104 (MQ=255)
                                                                                           gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                       >  2:355354/1‑149 (MQ=255)
                                                                                           gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGGCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATTCTgg                                                       <  4:64936/149‑1 (MQ=255)
                                                                                            ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACTCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                      >  1:88735/1‑149 (MQ=255)
                                                                                            ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                      <  7:260802/149‑1 (MQ=255)
                                                                                                        gagCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCCCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGc                                          <  5:308612/149‑1 (MQ=255)
                                                                                                         agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                         <  5:23629/149‑1 (MQ=255)
                                                                                                           cagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCAt                                        >  3:286456/1‑148 (MQ=255)
                                                                                                               tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCAGATCTGTACAGCATGCCGCAGCATGCTGCAGACCTGATCCTGGGGCAAAGAACCGCATGAAGc                                   >  8:8472/1‑149 (MQ=255)
                                                                                                                   cGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGat                               >  1:353359/1‑149 (MQ=255)
                                                                                                                    ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                     >  7:327227/1‑126 (MQ=255)
                                                                                                                    ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                     <  8:327227/126‑1 (MQ=255)
                                                                                                                         tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTg                           <  4:191911/147‑1 (MQ=255)
                                                                                                                             cAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGc                         <  5:237095/145‑1 (MQ=255)
                                                                                                                                    tGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa              <  2:88735/149‑1 (MQ=255)
                                                                                                                                     gCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa              >  3:213830/1‑148 (MQ=255)
                                                                                                                                         cgcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGc         >  8:100678/4‑149 (MQ=255)
                                                                                                                                          gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                   <  4:80502/120‑1 (MQ=255)
                                                                                                                                          gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                   >  3:80502/3‑122 (MQ=255)
                                                                                                                                             aCCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCtgt     <  7:100678/149‑1 (MQ=255)
                                                                                                                                                cGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGCCGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg  >  7:125396/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTGGTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGG  >  NC_002947/417159‑417451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑