Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,951,325	C→T	6.9%	P544P (CCG→CCA)	ppk ←	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,951,325	0	C	T	6.9%	171.1 / 2.3	58	P544P (CCG→CCA)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base T (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGACTGGCTGGCCTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGTGGTAGTTGC  >  NC_002947/5951181‑5951473                                                                                                                                                 |                                                                                                                                                      cGACTGGCTGGCCTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGgcg                                                                                                                                                   <  2:348855/149‑1 (MQ=255)       gctggcCTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATg                                                                                                                                               <  8:236472/147‑1 (MQ=255)            ccgtGTACAACGCCTAGATGACGTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCagcagc                                                                                                                                        <  8:118897/146‑1 (MQ=255)             cTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAATGGCGCATGCAGCAGct                                                                                                                                       >  1:267249/1‑149 (MQ=255)             cTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGct                                                                                                                                       >  2:11677/1‑149 (MQ=255)             cTTGTACAACCCCTTGATGACTTTGCCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAATGGCGCATGCAGCAGct                                                                                                                                       >  1:267254/1‑149 (MQ=255)              ttGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATg                                                                                                                                               >  7:76428/1‑140 (MQ=255)              ttGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATg                                                                                                                                               <  8:76428/140‑1 (MQ=255)                     aCGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATg                                                                                                                               <  8:23283/149‑1 (MQ=255)                         cTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATgcg                                                                                                                             >  3:188536/1‑147 (MQ=255)                              tgaCTTTGGCGTCGGTCACCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTc                                                                                                                      >  4:149186/1‑149 (MQ=255)                                 cTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTg                                                                                                                   <  1:11677/149‑1 (MQ=255)                                  tttGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCa                                                                                                                                 <  8:317089/134‑1 (MQ=255)                                                 cGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAATGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGGCCATGCCTA‑TCAgct                                                                                                  <  2:267254/149‑1 (MQ=255)                                                 cGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAATGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAgct                                                                                                  <  2:267249/149‑1 (MQ=255)                                                    gTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGt                                                                                                                       >  3:105564/1‑126 (MQ=255)                                                    gTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGt                                                                                                                       <  4:105564/126‑1 (MQ=255)                                                     ttGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATgcagca                                                                                                                                          >  8:229470/1‑106 (MQ=255)                                                     ttGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATgcagca                                                                                                                                          <  7:229470/106‑1 (MQ=255)                                                     ttGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAgctggct                                                                                              <  5:184324/149‑1 (MQ=255)                                                        aaCTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGaa                                                                                           <  4:12852/149‑1 (MQ=255)                                                             ggCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTGTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGc                                                                                       <  8:87606/148‑1 (MQ=255)                                                                       tgCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGt                                                                             <  4:285008/148‑1 (MQ=255)                                                                          gcCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTc                                                                         >  3:279741/1‑149 (MQ=255)                                                                          gcCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTc                                                                         >  2:254810/1‑149 (MQ=255)                                                                             ggCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACTGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCACCTGGCTGAACAGCTTGCCGACGTCTTCGGt                                                                      <  1:29380/149‑1 (MQ=255)                                                                                  gCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAgc                                                                  <  3:149186/148‑1 (MQ=255)                                                                                   ccTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCATCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCAGACGTCTTCGGTGAgcg                                                                 >  7:148563/1‑148 (MQ=255)                                                                                          gggCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGt                                                           >  2:308427/1‑147 (MQ=255)                                                                                           ggCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGc                                                                                                            <  3:280419/98‑1 (MQ=255)                                                                                           ggCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGc                                                                                                            >  4:280419/1‑98 (MQ=255)                                                                                             cAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGaa                                                      <  5:187894/149‑1 (MQ=255)                                                                                                aCTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTc                                                   <  4:188536/149‑1 (MQ=255)                                                                                                 cTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGt                                                    >  2:251291/1‑147 (MQ=255)                                                                                                               cgATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCCACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCg                                    <  1:251291/149‑1 (MQ=255)                                                                                                                 aTCCTGGCGAGCATGCCCTTTTTCAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGgt                                  <  4:279741/149‑1 (MQ=255)                                                                                                                  tCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGgtg                                 >  5:184924/1‑149 (MQ=255)                                                                                                                     tGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGaa                                                      >  3:190986/1‑125 (MQ=255)                                                                                                                     tGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGaa                                                      <  4:190986/125‑1 (MQ=255)                                                                                                                     tGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGACTGAACAGCTTGCCGACGTCTTCGGTGAGCGCAt                                                              <  6:260785/117‑1 (MQ=255)                                                                                                                     tGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGACTGAACAGCTTGCCGACGTCTTCGGTGAGCGCAt                                                              >  5:260785/1‑117 (MQ=255)                                                                                                                      gTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTa                               >  4:96532/1‑147 (MQ=255)                                                                                                                         gAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAggc                          >  8:334068/1‑149 (MQ=255)                                                                                                                           gCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAggcgg                        >  6:113673/1‑149 (MQ=255)                                                                                                                                cccTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGtt                   >  6:16224/1‑149 (MQ=255)                                                                                                                                cccTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGtt                   >  7:271200/1‑149 (MQ=255)                                                                                                                                accTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTGATGCTCTGTGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGtt                   <  8:148563/148‑1 (MQ=255)                                                                                                                                  cTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTGGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGc                 >  6:68355/1‑149 (MQ=255)                                                                                                                                   tttttAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGcc                >  7:330223/1‑149 (MQ=255)                                                                                                                                     tttAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCg               <  7:334068/148‑1 (MQ=255)                                                                                                                                     tttAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCg               <  8:271200/148‑1 (MQ=255)                                                                                                                                        aaGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTc                                                          <  5:336077/102‑1 (MQ=255)                                                                                                                                        aaGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTc                                                          >  6:336077/1‑102 (MQ=255)                                                                                                                                          tggTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGt           <  3:57679/146‑1 (MQ=255)                                                                                                                                           ggTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGTgg         >  6:193875/1‑148 (MQ=255)                                                                                                                                            gTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGAGTCAGCTGGCTGAACAGATTGCCGCCGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGTgg         >  1:93307/1‑148 (MQ=255)                                                                                                                                            gTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGt           >  4:57679/1‑145 (MQ=255)                                                                                                                                                aCGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGTGGTAGTTg   >  6:226178/1‑149 (MQ=255)                                                                                                                                                 cGGCGCAGGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGCGCGTTGCCGGCGTGGTAGTTGc  <  7:226340/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      CGACTGGCTGGCCTTGTACAACGCCTTGATGACTTTGGCGTCGGTCAGCGAGTTGAACTTGGCGATGATGTGCGCCGGCTTGCCTTCAAGGGCAAACTGGGTTTCCCGCGCGATCATGTCGAGCATGCCCTTTTTAAGGGTGAACGGCGCATGCAGCAGCTTCTTCATGCGCAGGGTCTTGCCCATGCCGA‑TCAGCTGGCTGAACAGCTTGCCGACGTCTTCGGTGAGCGCATCGTCAGAAGTCAGCAGGCTGTAGTCGGTGTACAGGCGGGCGTTGCCGGCGTGGTAGTTGC  >  NC_002947/5951181‑5951473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑