Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,960,282 G→C 7.8% P8A (CCG→GCG)  PP_5224 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,960,2820GC 7.8% 150.1 / 4.1 51P8A (CCG→GCG) PP_5224hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base C (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

ATCGGCCGAGCGCCATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTTCGTCGTAA  >  NC_002947/5960145‑5960422
                                                                                                                                         |                                                                                                                                            
aTCGGCCGAGCGCCATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTgg                                                                                                                                    <  4:143393/148‑1 (MQ=255)
 tCGGCCGAGCGGCATTGGCGTTTGTGCTCGACATGCAGTTGGAAAACGGTGAACACGCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGAGGGCACTGCAGGGCTGACGTTGGTGTAATGCGGCTTGGGCGGCCGGGCCTGGgc                                                                                                                                  <  2:5907/149‑1 (MQ=255)
   ggCCGAGCGCCATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGATCATCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGgcgc                                                                                                                                <  3:249311/149‑1 (MQ=255)
             cATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGt                                                                                                                       >  3:122802/1‑148 (MQ=255)
             cATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGCCCAGGCCCGGccgccca                                                                                                                             <  4:337745/142‑4 (MQ=255)
             cATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGCCCAGGCCCGGccgccca                                                                                                                             >  3:337745/1‑139 (MQ=255)
                   cgAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGCCCAGGCCCGGccgcccaagc                                                                                                                          <  1:236541/139‑7 (MQ=255)
                   cgAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGCCCAGGCCCGGccgcccaagc                                                                                                                          >  2:236541/1‑133 (MQ=255)
                          gCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTtcgtcg                                                                                                           >  2:200939/1‑147 (MQ=255)
                           cTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAg                                                                                                        <  1:200939/149‑1 (MQ=255)
                            tCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCAAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGt                                                                                                       >  5:99342/1‑149 (MQ=255)
                              gACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTc                                                                                                      >  4:40357/1‑148 (MQ=255)
                               aCATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTc                                                                                                      >  6:311804/1‑147 (MQ=255)
                                    ccGATGGCAACCGGTGCACACCTTTTGTAATTCCAGCCGGCATACGGTGAAGCACGGTGACGGCAATGCAGGGCTGAGGTTGATGTAAAGCGGCTGGGCCGGCCGGGCGTGGGCGCTACTCATGAAAGATTTCGACGAAGACCAgataa                                                                                               >  7:55088/1‑145 (MQ=255)
                                    ccGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCGGCTtc                                                                                               >  2:239043/1‑149 (MQ=255)
                                      gATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTtctc                                                                                             >  3:187659/1‑149 (MQ=255)
                                          gCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCg                                                                                         <  4:187659/149‑1 (MQ=255)
                                           cAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCgg                                                                                        >  8:264719/1‑149 (MQ=255)
                                            aaCCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGc                                                                                       >  5:4375/1‑149 (MQ=255)
                                               cGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCg                                                                                    <  4:122802/149‑1 (MQ=255)
                                                 gTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCGGCTTCTCGCCGGCCCGCt                                                                                  <  1:239043/149‑1 (MQ=255)
                                                    cacaCCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTcc                                                                                >  5:179788/1‑148 (MQ=255)
                                                       acCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGa                                                                            <  5:311804/149‑1 (MQ=255)
                                                       acCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGa                                                                            >  5:186892/1‑149 (MQ=255)
                                                       acCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTAGCCGGCCCGCTCCCAGa                                                                            >  8:283034/1‑149 (MQ=255)
                                                         ccGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCa                                                                                                                     >  2:232659/1‑106 (MQ=255)
                                                         ccGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCa                                                                                                                     <  1:232659/106‑1 (MQ=255)
                                                                     cAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAg                                                               <  5:83226/148‑1 (MQ=255)
                                                                     cAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAg                                                               <  6:179788/148‑1 (MQ=255)
                                                                     cAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAg                                                               <  1:307906/148‑1 (MQ=255)
                                                                       gCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCAt                                                            >  6:127033/1‑149 (MQ=255)
                                                                        ccGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCAtt                                                           >  8:60695/1‑149 (MQ=255)
                                                                           gCATACGATGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGTACCGCTCCCCGACGAAACGCTCGAGCATGTcc                                                        >  8:83083/1‑149 (MQ=255)
                                                                             aTACGCTGATGCACGGTGTCGGCACTGCATGGCTGACGTTGCTGTAAATCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTcccc                                                      <  6:99342/149‑1 (MQ=255)
                                                                             aTACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTcccc                                                      >  6:338731/1‑149 (MQ=255)
                                                                               aCGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTcccc                                                      >  4:65723/1‑147 (MQ=255)
                                                                                     aTGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAg                                                               <  8:58685/132‑1 (MQ=255)
                                                                                                 ggCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCtttt                                  >  2:206500/1‑149 (MQ=255)
                                                                                                   cACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCg                                <  7:264719/149‑1 (MQ=255)
                                                                                                    aCTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGc                               <  3:40357/149‑1 (MQ=255)
                                                                                                      tGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGca                              >  7:217186/1‑148 (MQ=255)
                                                                                                                aCGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACtt                     <  1:158070/147‑1 (MQ=255)
                                                                                                                aCGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTg                    <  3:65723/148‑1 (MQ=255)
                                                                                                                  gTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGcag                 >  8:119294/1‑147 (MQ=255)
                                                                                                                     gCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCt               <  5:338731/148‑1 (MQ=255)
                                                                                                                         tAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCt           >  8:136790/1‑148 (MQ=255)
                                                                                                                           aaGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTtc         <  8:55088/148‑1 (MQ=255)
                                                                                                                            aGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTtcgt       <  8:217186/149‑1 (MQ=255)
                                                                                                                            aGCGGCTTGGGCGGCAGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCACAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTtcgt       <  7:83083/149‑1 (MQ=255)
                                                                                                                                gCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTGTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTTcgtcgt    >  7:93741/1‑148 (MQ=255)
                                                                                                                                  ttGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCCAGCATTTCCCCCAGCAACTCATACCTCTTTTCTCACACCCACTTGCCGCTGTCTTCGTCGTaa  >  1:298710/1‑148 (MQ=255)
                                                                                                                                      gcggcCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTtcgtc      <  7:31969/140‑1 (MQ=255)
                                                                                                                                      gcggcCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTtcgtc      >  8:31969/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ATCGGCCGAGCGCCATTCGCGAATGTGCTCGACATGCCGATGGCAACCGGTGCACACCCGTTGTTCGTCCAGCCGGCATACGCTGATGCACGGTGACGGCACTGCAGGGCTGACGTTGCTGTAAAGCGGCTTGGGCGGCCGGGCCTGGGCGCTACTCATGTCAGATTTCGTCGAAGTCCAGCTTCTCGCCGGCCCGCTCCCAGACGATACGCTCGAGCATTTCCCCCAGCAACTCTTCGCTCTTTTCGCACACCCACTTGCCGCTGTCTTCGTCGTAA  >  NC_002947/5960145‑5960422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑