Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 417,310 G→A 7.8% A340A (GCG→GCA)  waaG → lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947417,3100GA 7.8% 145.5 / 2.4 51A340A (GCG→GCA) waaGlipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base A (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGG  >  NC_002947/417164‑417456
                                                                                                                                                  |                                                                                                                                                  
gTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGcgcg                                                                                                                                                  >  8:183011/1‑149 (MQ=255)
 tCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGt                                                                                                                                                 >  6:23629/1‑149 (MQ=255)
 tCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGt                                                                                                                                                 <  8:333652/149‑1 (MQ=255)
     cGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCa                                                                                                                                             <  7:183011/149‑1 (MQ=255)
              ggTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTcgc                                                                                                                                    <  6:216697/149‑1 (MQ=255)
                   tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                               >  1:218119/1‑149 (MQ=255)
                   tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                               >  8:71507/1‑149 (MQ=255)
                     gtgtCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATgg                                                                                                                             >  3:64936/1‑149 (MQ=255)
                                 tgCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGc                                                                                                                 >  4:304703/1‑149 (MQ=255)
                                   cGGTTATGCCCACTACATTGCCGAGGCCGACAGCTGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTTCCAGCTGGTCGCGCAATGGCCTGGCATTTGCt                                                                                                                <  3:263394/148‑1 (MQ=255)
                                    ggTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGa                                                                                                              <  7:71507/149‑1 (MQ=255)
                                      ttATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATa                                                                                                            <  3:123146/149‑1 (MQ=255)
                                            ccACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGcc                                                                                                       >  1:250589/1‑148 (MQ=255)
                                              aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGa                                                                                                     >  3:191911/1‑148 (MQ=255)
                                              aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGAt                                                                                                    <  2:88683/149‑1 (MQ=255)
                                               cTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATc                                                                                                   >  6:308612/1‑149 (MQ=255)
                                                   aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                       >  7:125649/1‑125 (MQ=255)
                                                   aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                       <  8:125649/125‑1 (MQ=255)
                                                   aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTa                                                                                               <  2:218119/149‑1 (MQ=255)
                                                      gCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACa                                                                                             <  1:243912/148‑1 (MQ=255)
                                                         gAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCAt                                                                                         >  1:148218/1‑149 (MQ=255)
                                                              cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgc                                                                                    >  4:248031/1‑149 (MQ=255)
                                                              cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCg                                                                                     >  6:237095/1‑148 (MQ=255)
                                                                  aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                         >  5:154557/1‑124 (MQ=255)
                                                                  aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                         <  6:154557/124‑1 (MQ=255)
                                                                   gCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAt                                                                               <  2:250589/149‑1 (MQ=255)
                                                                         tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                           >  8:326191/1‑147 (MQ=255)
                                                                         tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                           <  7:326191/147‑1 (MQ=255)
                                                                            tGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCACAGCATGCTGCCGAc                                                                      <  5:153217/149‑1 (MQ=255)
                                                                                      gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGCCCCGCAGGCGCGTGCCCGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGAACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                            >  3:20207/1‑149 (MQ=255)
                                                                                      gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                         <  8:211476/104‑1 (MQ=255)
                                                                                      gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                         >  7:211476/1‑104 (MQ=255)
                                                                                      gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                            >  2:355354/1‑149 (MQ=255)
                                                                                      gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGGCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATTCTgg                                                            <  4:64936/149‑1 (MQ=255)
                                                                                       ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACTCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                           >  1:88735/1‑149 (MQ=255)
                                                                                       ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                           <  7:260802/149‑1 (MQ=255)
                                                                                                   gagCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCCCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGc                                               <  5:308612/149‑1 (MQ=255)
                                                                                                    agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                              <  5:23629/149‑1 (MQ=255)
                                                                                                      cagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCAt                                             >  3:286456/1‑148 (MQ=255)
                                                                                                          tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCAGATCTGTACAGCATGCCGCAGCATGCTGCAGACCTGATCCTGGGGCAAAGAACCGCATGAAGc                                        >  8:8472/1‑149 (MQ=255)
                                                                                                              cGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGat                                    >  1:353359/1‑149 (MQ=255)
                                                                                                               ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                          >  7:327227/1‑126 (MQ=255)
                                                                                                               ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                          <  8:327227/126‑1 (MQ=255)
                                                                                                                    tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTg                                <  4:191911/147‑1 (MQ=255)
                                                                                                                        cAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGc                              <  5:237095/145‑1 (MQ=255)
                                                                                                                               tGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa                   <  2:88735/149‑1 (MQ=255)
                                                                                                                                gCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa                   >  3:213830/1‑148 (MQ=255)
                                                                                                                                    cgcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGc              >  8:100678/4‑149 (MQ=255)
                                                                                                                                     gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                        <  4:80502/120‑1 (MQ=255)
                                                                                                                                     gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                        >  3:80502/3‑122 (MQ=255)
                                                                                                                                        aCCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCtgt          <  7:100678/149‑1 (MQ=255)
                                                                                                                                           cGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGCCGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg       >  7:125396/1‑149 (MQ=255)
                                                                                                                                                gcgcGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCggg  >  7:250514/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTCGCCGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGG  >  NC_002947/417164‑417456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑